Role metabolismu, signálních molekul a buněčných struktur při stárnutí, stresu a adaptaci

Role metabolismu, signálních molekul a buněčných struktur při stárnutí, stresu a adaptaci

RNDr. Libuše Váchová, CSc.

RNDr. Libuše Váchová, CSc. — Vedoucí projektu

Mikrobiologický ústav AV ČR

O nás

The project focuses on molecular processes and regulatory mechanisms involved in the adaptation, aging and cell death of yeast populations and in the response of populations to different stresses. The project focuses mainly on multicelluar populations such as biofilms and colonies that are capable of differentiation.

The main topics currently studied included identification of specific metaboli processes and defense strategies important for long-term developoment of colonies and biofilms and different roles of celluar organeles (e. g. mitochondria, peroxisomes and autophagosomes). Different approaches are used in the studies that include, in addition to a wide spectrum of methods of microbiology, biochemistry and cell and molecular biology, unique techniques developed in the laboratory for investigating yeast populations. These techniques include, for example, specific approaches of two photon confocal microscopy. The research in the project takes advantage from of the close collaboration with project 2. 1. 5., which also includes collaboration with the G. Gilfillan group in Oslo, Norway of Czech-Norwegian grant funding.

Potential for cooperation

The expertise that we offer involves physiology and cell biology of yeast populations, including an analysis of selected stress defense processes. We also offer cooperation in the field of immobilized yeast populations covered by our patent "Modification method of detection yeast strain" No 305223, which enables detection of different chemicals that have been applied in Disposable kit for detection of the content of cupric (Cu2+) ions in drinking waters and industrial wastewaters developed with LentiKat's a.s.




Váchová L., Plocek V., Maršíková J., Rešetárová S., Hatáková L., Palková Z. (2024) Differential stability of Gcn4p controls its cell-specific activity in differentiated yeast colonies. mBio 15(5): e00689-24, doi:10.1128/mbio.00689-24


Tate J., Marsikova J., Vachova L., Palkova Z, Cooper T.G. (2022) Effects of abolishing Whi2 on the proteome and nitrogen catabolite repression-sensitive protein production. G3: Genes|Genomes|Genetics 12(3): jkab432 doi: 10.1093/g3journal/jkab432


Plocek V., Fadrhonc K., Maršíková J., Váchová L., Pokorná A., Hlaváček O., Wilkinson D., Palková Z. (2021) Mitochondrial retrograde signaling contributes to metabolic differentiation in yeast colonies. International Journal of Molecular Sciences 22: 5597, DOI: 10.3390/ijms22115597

Palková Z., Váchová L. (2021) Spatially structured yeast communities: Understanding structure formation and regulation with omics tools. Computational and Structural Biotechnology Journal 19: 5613-5621,


Van Nguyen P., Plocek V., Váchová L., Palková Z. (2020) Glucose, Cyc8p and Tup1p regulate biofilm formation and dispersal in wild Saccharomyces cerevisiae, npj Biofilms and Microbiomes 6:7; (

Plocek V., Váchová L., Šťovíček V., Palková Z. (2020) Cell distribution within yeast colonies and colony biofilms: How structure develops. International Journal of Molecular Sciences 21: 3873; doi:10.3390/ijms21113873

Maršíková J., Pavlíčková M., Wilkinson D., Váchová L., Hlaváček O., Hatáková L., Palková Z. (2020) The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in yeast colonies. PNAS first published June 15, 2020


Vachova L., Palkova Z. (2019) Diverse roles of Tup1p and Cyc8p transcription regulators in the development of distinct types of yeast populations. Current Genetics 65(1): 147-151


Wilkinson D., Maršíková J., Hlaváček O., Gilfillan GD., Ježková E., Aaløkken R., Váchová L., Palková Z. (2018) Transcriptome remodeling of differentiated cells during chronological ageing of yeast colonies: New insights into metabolic differentiation. Oxidative Medicine and Cellular Longevity, Volume 2018, Article ID 4932905

Vachova L., Palkova Z. (2018) How structured yeast multicellular communities live, age and die? FEMS Yeast Research 18, 2018, foy033, 1-9

Segorbe, D., Wilkinson D., Mizeranschi A., Hughes T., Aaløkken R., Vachova L., Palkova Z., D. Gilfillan G.D. (2018) An optimized FAIRE procedure for low cell numbers in yeast. Yeast 2018: 1-6 doi: 10.1002/yea.3316

Aouacheria A, Cunningham, KW., Hardwick, JM., Palková, Z., Powers, T., Severin, FF., Váchová, L., Comment on “Sterilizing immunity in the lung relies on targeting fungal apoptosis-like programmed cell death”, Science 360 (6395): eaar6910, DOI: 10.1126/science.aar6910

Van Nguyen P., Hlavacek O., Marsikova J., Vachova L., Palkova Z. (2018) Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity. PLoS genetics,

Wilkinson D., Váchová L., Hlaváček O., Maršíková J., Gilfillan G.D., Palková Z. (2018) Long non-coding RNAs in yeast cells and differentiated subpopulations of yeast colonies and biofilms. Oxidative Medicine and Cellular Longevity, Volume 2018, Article ID 4950591,


Hovsepian J., Defenouillère Q., Albanèse V., Váchová L., Garcia C., Palková Z., Léon S. (2017) Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis. J. Cell Biol. 216(6): 1811-1831

Maršíková J., Wilkinson D., Hlaváček O., Gilfillan G., Mizeranschi A., Hughes T., Begany M., Rešetárová S., Váchová L., Palková Z. (2017) Metabolic differentiation of surface and invasive cells of yeast colony biofilms revealed by gene expression profiling. BMC Genomics (2017) 18:814, DOI 10.1186/s12864-017-4214-4


Palkova Z, Vachova L (2016) Yeast cell differentiation: Lessons from pathogenic and non-pathogenic yeasts. Semin Cell Dev Biol. 57: 110-9.

Podholova K, Plocek V, Resetarova S, Kucerova H, Hlavacek O, Vachova L, Palkova Z (2016) Divergent branches of mitochondrial signaling regulate specific genes and the viability of specialized cell types of differentiated yeast colonies. Oncotarget 7: 15299-314.

Kuznetsov E., Váchová L., Palková Z. (2016) Cellular localization of Sun4p and its interaction with proteins in the yeast birth scar. Cell Cycle 15(14): 1898-1907

Palková Z., Váchová L. (2016) Mitochondria in aging cell differentiation. Aging-US 8(7): 1287-1288

Klionsky D.J. et al (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12(1): 1-222, DOI:10.1080/15548627.2015.1100356


Vopálenská I., Váchová L., Palková Z. (2015) New biosensor for detection of copper ions in water based on immobilized genetically modified yeast cells. Biosensors and Bioelectronics 72: 160–167

Čáp M., Váchová L, Palková Z. (2015) Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis. Cell Cycle 14: 21, 3488-3497


Palkova, Z, Wilkinson, D, Vachova, L (2014) Aging and differentiation in yeast populations: Elders with different properties and functions. FEMS Yeast Res 14: 96-108

Šťovíček V., Váchová L., Begany M., Wilkinson D., PalkováZ. (2014) Global changes in gene expression associated with phenotypic switching of wild yeast. BMC Genomics 15: Art. No. 136, DOI: 10.1186/1471-2164-15-136


Kuznetsov E,, Kučerová H,, Váchová L,, Palková Z, (2013) SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level. PLoS One. 2013 Sep 11;8(9):e73882. doi: 10.1371/journal.pone.0073882

Paiva S., Strachotová D., Kučerová H., Hlaváček O., Mota S., Casal M., Palková Z., Váchová L. (2013) The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies. Biochem. J. 454 (3): 551-558

Váchová L., Hatáková L., Čáp M., Pokorná M., Palková Z. (2013) Rapidly Developing Yeast Microcolonies Differentiate in a Similar Way to Aging Giant Colonies. Oxidative Medicine and Cellular Longevity, Article Number: UNSP 102485, doi:  10.1155/2013/102485


Cottier F, Raymond M, Kurzai O, Bolstad M, Leewattanapasuk W, Jiménez-López C, Lorenz MC, Sanglard D, Váchová L, Pavelka N, Palková Z, Mühlschlegel FA.: The bZIP transcription factor Rca1p is a central regulator of a novel CO2 sensing pathway in yeast. PLoS Pathogens 2012; 8(1): e1002485

Čáp M, Štěpánek L, Harant K, Váchová L, Palková Z.: Cell differentiation within a yeast colony: metabolic and regulatory parallels with a tumor-affected organism. Molecular Cell 2012; 46: 436-48.

Váchová L., Čáp M., Palková Z. (2012) Yeast Colonies: A Model for Studies of Aging, Environmental Adaptation, and Longevity. Oxidative Med. Cell. Longev. Article Number: 601836   DOI: 10.1155/2012/601836

Šťovíček V., Váchová L., Palková Z. (2012) Yeast biofilm colony as an orchestrated multicellular organism. Commun Integr Biol 5: 203-205

Pineda-Rodó A., Váchová L., Palková Z. (2012) In vivo determination of organellar pH using a universal wavelength-based confocal microscopy approach, PLOS One 7: e33229, doi:10.1371/journal.pone. 0033229

Palkova Z., Vachova L. (2012) Communication and differentiation in the development of yeast colonies, In: Biocommunication in Fungi, Edited by Guenther Witzany, Springer 2012, pp 141-154

Čáp M., Váchová L., Palková Z. (2012) Reactive oxygen species in the signaling and adaptation of multicellular microbial communities. Oxidative Med. Cell. Longev. Volume 2012, Article ID 976753, doi:10.1155/2012/976753

Strachotová D., Holoubek A., Kučerová H., Benda A., Humpolíčková J., Váchová L., Palková Z. (2012) Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM). BBA-Biomembranes 1818: 2126-2134 


Váchová L, Šťovíček V, Hlaváček O, Chernyavskiy O, Štěpánek L, Kubínová L, Palková Z.: Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies. Journal of Cell Biology 2011; 194: 679-87

Vachova L., Palkova Z. (2011) Aging and longevity of yeast colony populations: metabolic adaptation and differentiation. Biochem Soc Trans. 39: 1471-5


Vopalenska I., Stovicek V., Janderova B., Vachova L., Palkova Z. (2010) Role of distinct dimorphic transitions in territory colonizing and formation of yeast colony architecture. Environ Microbiol 12: 264-77

Stovicek V., Vachova L., Kuthan M., Palkova Z. (2010) General factors important for the formation of structured biofilm-like yeast colonies. Fungal Genet Biol. 47: 1012-22

Cap M., Vachova L., Palkova Z. (2010) How to survive within a yeast colony: Change metabolism or cope with stress? Commun Integr Biol 3: 198-200


Vachova L., Kucerova H., Devaux F., Ulehlova M., Palkova Z. (2009) Metabolic diversification of cells during the development of yeast colonies. Environ. Microbiol. 11: 494-504

Vachova L., Chernyavskiy O., Strachotová D., Bianchini P., Burdiková Z., Ferciková I., Kubinova L., Palkova Z. (2009) Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter. Environ Microbiol. 11: 1866-1877

Palkova Z., Vachova L., Gaskova D., Kucerova H. (2009) Synchronous plasma membrane electrochemical potential oscillations during yeast colony development and aging. Mol Membr Biol. 26: 228-35

Hlavacek O., Kucerova H., Harant K., Palková Z., Vachová L. (2009) Putative role for ABC multidrug exporters in yeast quorum sensing. FEBS Lett. 583: 1107-13

Cap M., Vachova L., Palkova Z. (2009) Yeast colony survival depends on metabolic adaptation and cell differentiation rather than on stress defense. J Biol Chem. 284: 32572-81


Váchová L., Palková Z. (2007) Caspases in yeast apoptosis-like death: facts and artefacts. FEMS Yeast Res. 7: 12-21

Ricicova M., Kucerova H, Vachova L., Palkova Z. (2007) Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localization in patches. BBA-Biomembranes, 1768: 1170-8


Palková Z., Váchová L. (2006) Life within a community: benefit to yeast long-term survival. FEMS Microbiol Rev 30: 806–824


Vachova L., Palkova Z. (2005) Physiological regulation of yeast cell death in multicellular colonies is triggered by ammonia. J Cell Biol. 169: 711-7


Vachova L., Devaux F., Kucerova H., Ricicova M., Jacq C., Palkova Z. (2004) Sok2p transcription factor is involved in adaptive program relevant for long-term survival of Saccharomyces cerevisiae colonies. J Biol Chem. 279:  37973-81

Palková Z., Váchová L., Valer M., Preckel T. (2004) Single cell analysis of yeast, mammalian cells and fungal spores with a microfluidic pressure driven chip-based system. Cytometry, part A 59: 246-253


Palková Z., Vachova L. (2003) Ammonia signalling in yeast colony formation. Int. Rev. Cytol., 225: 229 – 272, Invited review


RNDr. Libuše Váchová, CSc. RNDr. Libuše Váchová, CSc.

Libuše Váchová, CSc.

Vedoucí skupiny Role metabolismu, signálních molekul a buněčných struktur při stárnutí, stresu a adaptaci
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