Gene expression: 9216 qPCR rections with GE 96.96 or 2304 qPCR reactions with GE 48.48
High-throughput qPCR System BioMark (Fluidigm) uses integrated fluidic circuits known as dynamic arrays and digital arrays. These innovative products allow scientists to practice tried-and-true techniques, such as TaqMan assays or EvaGreen chemistry, while realizing a previously unachievable throughput. Researchers save on reagents, pipette tips, time, and on the upkeep of expensive liquid-handling robots.
Using BioMark you can save:
- Time – the whole experiment is performed in one day
- Material –2 ul of sample is enough for 96 gene analysis
- Mastermix – instead of 46 ml of mastermix during regular qPCR, 240 ul of mastermix is enough
- Primers and probes – instead of 4.6 ml for all primers, 240 ul is sufficient (2.5 ul for 1 pair)
- Tips – instead of 18432 pipet steps, you will pipette only 196 x
- Plates – instead of 24 PCR plates (384 wells), 1 Biomark array will do the work
- Interplate calibration – if your whole experiment fits into one array, than you do not need interplate calibration
- Work – we will do the whole experiment for you
- Money – the whole experiment is cheaper than the regular experiment using 384 well PCR plates
ADDITIONAL RESOURCES: https://www.fluidigm.com/publications/biomark
Fluidigm GE arrays available at CF
96.96 Dynamic Array IFC for Gene Expression: combines 96 samples with 96 assays (9216 reactions)
48.48 Dynamic Array IFC for Gene Expression: combines 48 samples with 48 assays (2304 reactions)
Flex Six™ IFC for Gene Expression: 6 x reusable chip that combines 12 samples with 12 assays (6x 144 reactions)
Genotyping: 9216 qPCR reactions with GT 96.96 or 2304 qPCR reactions with GT 48.48
High-throughput qPCR System BioMark (Fluidigm) uses integrated fluidic circuits known as dynamic arrays and digital arrays. These innovative products allow scientists to practice tried-and-true techniques, while realizing a previously unachievable throughput. Researchers save on reagents, pipette tips, time, and on the upkeep of expensive liquid-handling robots.
Using BioMark you can save:
- Time – the whole experiment is performed in one day
- Material –2 ul of sample is enough for 96 gene analysis
- Mastermix – instead of 46 ml of mastermix during regular qPCR, 240 ul of mastermix is enough
- Primers and probes – instead of 4.6 ml for all primers, 240 ul is sufficient (2.5 ul for 1 pair)
- Tips – instead of 18432 pipet steps, you will pipette only 196 x
- Plates – instead of 24 PCR plates (384 wells), 1 Biomark array will do the work
- Interplate calibration – if your whole experiment fits into one array, than you do not need interplate calibration
- Work – we will do the whole experiment for you
- Money – the whole experiment is cheaper than the regular experiment using 384 well PCR plates
Fluidigm GE arrays available at CF:
- 96.96 Dynamic Array IFC for Genotyping: combines 96 samples with 96 assays (9216 reactions)
- 48.48 Dynamic Array IFC for Genotyping: combines 48 samples with 48 assays (2304 reactions)
- Flex Six™ IFC for Genotyping: 6 x reusable chip that combines 12 samples with 12 assays (6x 144 reactions)
Available formats of chips:
- 12.765 Digital Array IFC: for dPCR of 12 samples
- 48.770 Digital Array IFC: for dPCR of 48 samples
ADDITIONAL RESOURCES: https://www.fluidigm.com/search?query=genotyping
Preamplification
Preamplification is specific, exponential amplification of a template, which is used for low concentrated samples and for high-throughput systems as BioMark. Because from one sample is measured amplification of 96 targets, the input concentration of DNA or cDNA needs to be high. The necessary concentration is not possible to reach with reverse transcription without the possibility of inhibition.
We offer preamplification of your samples using specific multiplex PCR that combines all your specific primers in a limited concentration and limited numbers of PCR cycles. We can also offer the validation of preamplification, see the figure.
ADDITIONAL RESOURCES:
http://genexp.ibt.cas.cz/189.pdf
http://genexp.ibt.cas.cz/192.pdf
