Summer School – Biology of Parasitic Protists: Practical Course
Research — 25.07.2022

Mechanisms for Transport of Proteins through Mitochondrial and Bacterial Membranes

Summer School – Biology of Parasitic Protists: Practical Course

Intensive crash-course from in silico hints to hard wet-lab mined data recovered using following methods:

  • Plasmid preparation (PCR, molecularcloning, sequencing, restriction digest)
  • Prokaryotic and eukaryotictransformation (heat shock, electroporation, nucleofection)
  • Transformants assessment (colony PCR,qRT-PCR, cellular cloning)
  • Phenotype testing (Western blotting,protein protection assay, immunofluorescent confocal microscopy, live imaging, superresolution (SIM) microscopy)
  • CRISPR/Cas9 based gene knock out

Anaerobic model parasites:

Trichomonas vaginalis
Giardia intestinalis

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