Mechanisms for Transport of Proteins through Mitochondrial and Bacterial Membranes

Mechanisms for Transport of Proteins through Mitochondrial and Bacterial Membranes

Mgr. Pavel Doležal, Ph.D.

Mgr. Pavel Doležal, Ph.D. — Project head

The Faculty of Science of the Charles University

About us

Our lab studies the mitochondrial biogenesis and the protein transport across biological membranes.

In particular, we are interested in

  • the extremely reduced mitochondrial forms known as mitosomes and hydrogenosomes,
  • protein transport across mitochondrial and bacterial membranes. Our general goal is
  • to understand the events, which enabled the transformation of the bacterial endosymbiont into mitochondria.





Leger MM, Petrů M, Žárský V, Eme L, Vlček Č, Harding T, B. Lang F, Eliáš M, Doležal P, Roger AJ. An ancestral bacterial division system is widespread in eukaryotic mitochondria. Proc Natl Acad Sci U S 2015

King NP, Newton P, Schuelein R, Brown DL, Petru M, Zarsky V, Dolezal P, Luo L, Bugarcic A, Stanley AC, Murray RZ, Collins BM, Teasdale RD, Hartland EL, Stow JL. Soluble NSF attachment protein receptor molecular mimicry by a Legionella pneumophila Dot/Icm effector. Cell Microbiol. 2015


Zubacova Z, Novak L, Bublikova J, Vacek V, Fousek J, Ridl J, Tachezy J, Dolezal P, Vlcek C, Hampl V. The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system. PLoS One 2013


Zarsky V, Tachezy J, Dolezal P. Tom40 is likely common to all mitochondria. Curr Biol. 2012

Sutak R, Hrdy I, Dolezal P, Cabala R, Sedinová M, Lewin J, Harant K, Müller M, Tachezy J. Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis. FEBS J. 2012

Martincova E, Voleman L, Najdrova V, De Napoli M, Eshar S, Gualdron M, Hopp CS, Sanin DE, Tembo DL, Van Tyne D, Walker D, Marcincikova M, Tachezy J, Dolezal P. Live imaging of mitosomes and hydrogenosomes by HaloTag technology. PLoS One 2012

Dolezal P, Aili M, Naderer T, Tong J, Jiang J-H, Marobbio CMT, Lee SF, Schuelein R, Belluzzo S, Binova E, Mousnier, Frankel G, Giannuzzi G, Palmieri F, Gabriel K, Hartland EL and Lithgow T. Legionella secretes a novel carrier protein across four membranes into mitochondria. PLoS Pathogens 2012


Gebert N, Gebert M, Oeljeklaus S, von der Malsburg K, Stroud DA, Kulawiak B,Wirth C, Zahedi RP, Dolezal P, Wiese S, Simon O, Schulze-Specking A, Truscott KN, Sickmann A, Rehling P, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N,Wiedemann N. Dual Function of Sdh3 in the Respiratory Chain and TIM22 Protein Translocase of the Mitochondrial Inner Membrane. Mol Cell. 2011

Alcock F, Webb CT, Dolezal P, Hewitt V, Shingu-Vasquez M, Likic VA, Traven A, Lithgow T. A Small Tim Homohexamer in the Relict Mitochondrion of Cryptosporidium. Mol Biol Evol. 2011

Rada P, Doležal P, Jedelský PL, Bursac D, Perry AJ, Šedinová M, Smíšková K, Novotný M, Beltrán NC, Hrdý I, Lithgow T, Tachezy J. The core components of organelle biogenesis and membrane transport in the hydrogenosomes of Trichomonas vaginalis. PLoS One. 2011

Jedelský PL, Doležal P, Rada P, Pyrih J, Smíd O, Hrdý I, Sedinová M, Marcinčiková M, Voleman L, Perry AJ, Beltrán NC, Lithgow T, Tachezy J. The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis. PLoS One. 2011

Tong J, Dolezal P, Selkrig J, Crawford S, Simpson AG, Noinaj N, Buchanan SK, Gabriel K, Lithgow T. Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana. Mol Biol Evol. 2011


Likic VA, Dolezal P, Celik N, Dagley M, Lithgow T. Using hidden markov models to discover new protein transport machines. Methods Mol Biol. 2010

Dolezal P, Dagley MJ, Kono M, Wolynec P, Likić VA, Foo JH, Sedinová M, Tachezy J, Bachmann A, Bruchhaus I, Lithgow T. The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica. PLoS Pathog. 2010 


Mgr. Pavel Doležal, Ph.D. Mgr. Pavel Doležal, Ph.D.

Pavel Doležal, Ph.D.

Head of Group Mechanisms for Transport of Proteins through Mitochondrial and Bacterial Membranes
+420325873945, +420325873909