Center for Research of Pathogenicity and Virulence of Parasites

Center for Research of Pathogenicity and Virulence of Parasites

prof. RNDr. Jan Tachezy, Ph.D.

prof. RNDr. Jan Tachezy, Ph.D. — Project head

The Faculty of Science of the Charles University

About us

The aim of the project is to identify virulence factors of important parasites of humans and livestock and to assess their potential for the development of novel therapeutic and diagnostic approaches.




The proposed research activities will have performed at the Faculty of Science, Charles University, which includes the laboratories of the European Centre of Excellence BIOCEV and partner laboratories of the Institute of Parasitology of Biology Centre of the Czech Academy of Sciences in České Budějovice and newly established, laboratories at the Faculty of Science, University of Ostrava.

Project solves the absence of significant means for the treatment important infections caused by parasites. Used chemotherapeutics are usually toxic and against number of them has originated resistance without having available alternative chemotherapeutics. Significant problems are disease vectors as Ticks and Phlebotominaes.

Organizational-economic problems, which project solves, include typical research deficiencies in CR:

  • fragmentation of research activities
  • absence of some important modern instruments
  • lack of communication between the basic research and the application sphere
  • limited participation with international projects and cooperation

Project aim

The main objective of the proposed research program is to discover the virulence factors of important parasites of humans and livestock, and to determine their potential for the development of new therapeutic and diagnostic procedures.

The proposed research project activities are part of the program: "Virulence factors of parasites as targets for development of novel antiparasitic strategies" and logically structured into five subprograms. The knowledge gained will be used for the development of new therapeutic and diagnostic procedures. The results will be published in high quality scientific journals or patented.

The main goal is to combine the research efforts of the parasitological teams of the Charles University (BIOCEV, Dept of Parasitology), Parasitology Institute ASCR and the University of Ostrava and to create a team with a critical concentration of research capacities with high quality infrastructure and experienced support project management.

The specific aims include:

  • the acquisition of the missing state-of-the-art instruments for the applicant and the partners. The completion of the research infrastructure at the Charles University and the establishment of new laboratory for the evaluation of the antiparasitic targets (Drug Discovery), which will assist in bridging the gap between the basic research and the application sphere.
  • strengthening the cooperation with leading international institutions
  • strengthening the participation in the international research projects and grant competitions
  • involvement of prominent international researchers in the research team




Gahura O., Chauhan P., Zíková A. (2022) Mechanisms and players of mitoribosomal biogenesis revealed in trypanosomatids. Trends in Parasitology, vol. 38(12), 1053-1067.

Kotsyfakis S., Illiaki-Giannakoudaki E., Anagnostopoulos A., Papadokostaki E., Giannakoudakis K., Goumenakis M., Kotsyfakis M. (2022) The application of machine learning to imaging in hematological oncology: A scoping review. Front. Oncol. 12:1080988. doi: 10.3389/fonc.2022.1080988

Zhang P., Chen K., Svobodová M., Yang T., Lukeš J., Zhang J., Lun Z., Lai D. (2022) Does the fish-infecting Trypanosoma micropteri belong to Trypanosoma carassii? Folia Parasitologica 69.

Štěpánek O., Čmoková A., Procházková E., Grobárová V., Černý J., Slapničková M., Zíková A., Kovařík M., Baszczyňski O. (2022) Piperazine-modified ketoconazole derivatives show increased activity against fungal and trypanosomatid pathogens. ChemMedChem, vol. 17(21).

Jirků M., Kašparová A., Lhotská Z., Oborník M., Brožová K., Petrželková K. J., Samaš P., Kadlecová O., Stensvold C. R., Jirků K. (2022) A cross-sectional study on the occurrence of the intestinal protist, Dientamoeba fragilis, in the gut-healthy volunteers and their animals. Int. J. Mol. Sci., 23, 15407.

Horáková E., Lecordier L., Cunha P. et al. (2022) Heme-deficient metabolism and impaired cellular differentiation as an evolutionary trade-off for human infectivity in Trypanosoma brucei gambiense. Nat Commun 13, 7075.

Gahura O., Mühleip A., Hierro-Yap C. et al. (2022) An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases. Nat Commun 13, 5989.

Benz C., Müller N., Kaltenbrunner S., Váchová H., Vancová M., Lukeš J., Varga V., Hashimi H. (2022) Kinetoplastid-specific X2-family kinesins interact with a kinesin-like pleckstrin homology domain protein that localizes to the trypanosomal microtubule quartet. Molecular Microbiology, vol. 118(3).

Singh V., Hada R. S., Jain R., Vashistha M., Kumari G., Singh S., Sharma N., Bansal M., Poonam, Zoltner M., Caffrey C. R., Rathi B., Singh S. (2022) Designing and development of phthalimides as potent anti-tubulin hybrid molecules against malaria. European Journal of Medicinal Chemistry, vol. 239.

Lemke C., Jílková A., Ferber D., Braune A, On A., Johe P., Zíková A., et al. (2022) Two tags in one probe: Combining fluorescence- and biotin-based detection of the trypanosomal cysteine protease rhodesain. Chemistry – a European Journal.

Vaňková K., Doleželová E., Tloušťová E., Hocková D., Zíková A., Janeba Z. (2022) Synthesis and anti-trypanosomal evaluation of novel N-branched acyclic nucleoside phosphonates bearing 7-aryl-7-deazapurine nucleobase. European Journal of Medicinal Chemistry, vol. 239

Pilátová J., Pánek T., Oborník M. et al. (2022) Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J, 16, 2290-2294.

Medina J. M., Abbas M. N., Bensaoud C., Hackenberg M., Kotsyfakis M. (2022) Bioinformatic analysis of Ixodes ricinus long non-coding RNAs predicts their binding ability of host miRNAs. Int. J. Mol. Sci., 23, 9761.

Stanicka A., Cichy A., Bulantová J., Labecka A. M., Cmiel A. M., Templin J., Horák P., Zbikowska E. (2022) Thinking “outside the box”: The effect of nontarget snails in the aquatic community on mollusc-borne diseases. Science of The Total Environment, vol. 845.

Trentelman J. J. A., de Vogel F. A., Colstrup E., Šíma R., Coumou J., Koetsveld J., Klouwens M. J., Nayak A., Ersoz J., Barriales D., Tomás-Cortázar J., Narasimhan S., Hajdušek O., Anguita J., Hovius J. W. (2022) Identification of novel conserved Ixodes vaccine candidates; a promising role for non-secreted salivary gland proteins. Vaccine, vol. 40(52), 7593-7603.

Lukeš J., Kachale A., Votýpka J., Butenko A., Field M. C. (2022) African trypanosome strategies for conquering new hosts and territories: the end of monophyly? Trends in parasitology, vol. 38(9), 724-736.

Magri A., Bianchi C., Chmelová Ľ, Caffara M., Galuppi R., Fioravanti M., Yurchenko V., Kostygov A. Y. (2022) Roe deer (Capreolus capreolus) are a novel potential reservoir for human visceral leishmaniasis in the Emilia-Romagna region of northeastern Italy. International Journal for parasitology, vol 52(12), 745-750.

Procházková M., Füzik T., Grybchuk D., Yurchenko V., Plevka P. (2022) Virion structure of Leishmania RNA virus 1. Virology, vol. 577, 149-154

Barcyté D., Zátopková M., Němcová Y., Richtář M., Yurchenko T., Jaške K., Fawley K. P., Škaloud P., Ševčíková T., Fawley M. W., Eliáš M. (2022) Redefining Chlorobotryaceae as one of the principal and most diverse lineages of eustigmatophyte algae. Molecular phylogenetics and Evolution, vol. 177.

Belinky F., Byková A., Yurchenko V., Rogozin I. B. (2022). No evidence for widespread positive selection on double substitutions within codons in primates and yeasts. Front. Genet. 13:991249 doi: 10.3389/fgene.2022.991249

Saura A., Zakharova A., Klocek D., Gerasimov E. S., Butenko A., Macedo D. H., Serviené E. et al. (2022) Elimination of LRVs elicits different responses in Leishmania spp. mSphere, vol. 7(4).

Zoltner M., Field M. C. (2022) Microbe profile: Euglena gracilis: photogenic, flexible and hardy. Microbiology, vol. 168(9).

Vojtechova I., Machacek T., Kristofikova Z., Stuchlik A., Petrasek T. (2022) Infectious origin of Alzheimer’s disease: Amyloid beta as a component of brain antimicrobial immunity. PLoS Pathog 18(11): e1010929.

Kazamia E., Mach J., McQuaid J. B., Gao X., Coale T. H., Malych R., Camadro J. M., Lesuisse E., Allen A. E., Bowler C., Šuťák R. (2022) In vivo localization of iron starvation induced proteins under variable iron supplementation regimes in Phaeodactylum tricornutum. Plant Direct, vol. 6(12),

Zítek J., Füssy Z., Treitli S. C., Peña-Diaz P., Vaitová Z.,Zavadska D., Harant K., Hampl V. (2022) Reduced mitochondria provide an essential function for the cytosolic methionine cycle. Current biology, vol. 32(23), 5057-5068.e5

Votýpka J., Stříbrná E., Modrý D., Bryja J., Bryjová A., Lukeš J. (2022) Unexpectedly high diversity of trypanosomes in small sub-Saharan mammals. International Journal for parasitology, vol 52(10), 647-658.

Pondeville E., Failloux A. B., Simard F., Volf P., Crisanti A., Haghighat-Khah R. E., Busquets N., Abad F. X., Wilson A. J., Bellini R., Arnaud S. M., Kohl A., Veronesi E. (2022) Infravec2 guidelines for the design and operation of containment level 2 and 3 insectaries in Europe. Pathogens and Global Health DOI: 10.1080/20477724.2022.2108639

Messahel N. E., Benallal K. E., Halada P., Lafri I., Manseur H., Hakem A., Houali K., Harrat Z., Volf P., Dvořák V. (2022) Identification of blood source preferences and Leishmania infection in sand flies (Diptera: Psychodidae) in north-eastern Algeria. Veterinary parasitology, vol. 31, e100729

Zakharova A., Albanaz A. T. S., Opperdoes F. R., Škodová-Sveráková I., Zagirova D., Saura A., et al. (2022) Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content. PLoS Negl Trop Dis 16(6): e0010510.

Pacáková L, Harant K., Volf P., Leštinová T. (2022) Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis. Front. Cell. Infect. Microbiol. 12:1022448 doi: 10.3389/fcimb.2022.1022448

Tachezy J., Makki A., Hrdý I. (2022) The hydrogenosome of Trichomonas vaginalis. The Journal of eukaryotic microbiology, vol. 69(6).

Rada P., Hrdý I., Zdrha A., Narayanasamy R. K., Smutná T., Horáčková J., Harant K., Beneš V., Ong S. C., Tsai C. Y., Luo H. W., Chiu C. H., Tang P., Tachezy J. (2022) Double-stranded RNA viruses are released from Trichomonas vaginalis inside small extracellular vesicles and modulate the exosomal cargo. Front. Microbiol. 13:893692. doi: 10.3389/fmicb.2022.893692

Záhonová K., Treitli S. C., Le T. et al. (2022) Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics. BMC Biol 20, 56.

Smutná T., Dohnálková A., Šuťák R., Narayanasamy R. K., Tachezy J., Hrdý I. (2022) A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis. Current biology, vol. 32(1), 124-135

Zimmann N., Rada P., Žárský V., Smutná T., Záhonová K., Dacks J., Harant K., Hrdý I., Tachezy J. (2022) Proteomic analysis of Trichomonas vaginalis phagolysosome, lysosomal targeting, and unconventional secretion of cysteine peptidases. Molecullar and cellular proteomics, vol 21(1): e100174

George E. E., Tashyreva D., Kwong W. K., Okamoto N., Horák A., Husník F., Lukeš J., Keeling P. J. (2022). Gene transfer agents in bacterial endosymbionts of microbial eukaryotes. Genome biology and Evolution, vol. 14(7).

Tashyreva D., Simpson A. G. B., Prokopchuk G., Škodová-Sveráková I., Butenko A., Hammond M., George E. E., Flegontova O., Záhonová K., Faktorová D., Yabuki A., Horák A., Keeling P. J., Lukeš J. (2022) Diplonemids – A review on "new" flagellates on the oceanic block. Protist, vol 173(2), 125868.

Klejch T., Keough D. T., King G., Doleželová E., Česnek M., Buděšínský M., Zíková A., Janeba Z., Guddat L. W., Hocková D. (2022) Stereo-defined acyclic nucleoside phosphonates are selective and potent inhibitors of parasite 6-oxopurine phosphoribosyltransferases. J. Med. Chem. 65, 5, 4030–4057

Hammond M., Dorrell R. G., Speijer D., Lukeš J. (2022) Eukaryotic cellular intricacies shape mitochondrial proteomic complexity. BioEssays, vol 44(5)

Prokopchuk G., Korytář T., Juricová V. et al. (2022) Trophic flexibility of marine diplonemids - switching from osmotrophy to bacterivory. ISME J 16, 1409–1419.

Kuppannan A., Jiang Y. Y., Maier W., Liu C., Lang C. F., Cheng C. Y., et al. (2022) A novel membrane complex is required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila. PLoS Genet 18(5): e1010194.

Zíková A. (2022) Mitochondrial adaptations throughout the Trypanosoma brucei life cycle. The Journal of eukaryotic microbiology.

Hegedüsová E., Maršalová V., Kulkarni S., Paris Z. (2022) Trafficking and/or division: Distinct roles of nucleoporins based on their location within the nuclear pore complex. RNA Biology, 19:1, 650 661, doi: 10.1080/15476286.2022.2067711

Sharaf A., Vijayanathan M., Oborník M., Mozgová I. (2022) Phylogenetic profiling resolves early emergence of PRC2 and illuminates its functional core. Life Science Alliance. DOI: 10.26508/lsa.202101271

Medina J. M., Jmel M. A., Cuveele B., Gómez-Martín C., Aparicio-Puerta E., Mekki I., Kotál J., Martins L. A., Hackenberg M., Bensaoud C., Kotsyfakis M. (2022) Transcriptomic analysis of the tick midgut and salivary gland responses upon repeated blood-feeding on a vertebrate host. Front. Cell. Infect. Microbiol. 12:919786. doi: 10.3389/fcimb.2022.919786

Urbanová V., Kalinová E., Kopáček P., Šíma R. (2022) Experimental infection of mice and ticks with the human isolate of Anaplasma phagocytophilum NY-18. Pathogens, 820.

Guizzo M. G., Dolezelikova K., Neupane S. et al. (2022) Characterization and manipulation of the bacterial community in the midgut of Ixodes ricinus. Parasites Vectors 15, 248.

Abbas M. N., Chlastáková A., Jmel M. A., Iliaki-Giannakoudaki E., Chmelař J., Kotsyfakis M. (2022) Serpins in tick physiology and tick-host interaction. Front. Cell. Infect. Microbiol. 12:892770. doi: 10.3389/fcimb.2022.892770

Perner J., Hajdušek O., Kopáček P. (2022) Independent somatic distribution of heme and iron in ticks. Current opinion in insect science, vol 51, 100916

Winkelmann F., Gesell Salazar M., Hentschker C. et al. (2022) Comparative proteome analysis of the tegument of male and female adult Schistosoma mansoni. Sci Rep 12, 7569.

Vondráček O., Mikeš L., Talacko P., Leontovyč R., Bulantová J., Horák P. (2022) Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion. International Journal for parasitology, vol. 52(6), 343-358.

Grechnikova M., Arbon D., Ženíšková K., Malych R., Mach J., Krejbichová L., Šimáčková A., Šuťák R. (2022) Elucidation of iron homeostasis in Acanthamoeba castellanii. International Journal for parasitology, vol. 52(8), 497-508.

Ženíšková K., Grechnikova M., Šuťák R. (2022) Copper metabolism in Naegleria gruberi and its deadly relative Naegleria fowleri. Front. Cell. Dev. Biol. 10:853463. doi: 10.3389/fcell.2022.853463

Arbon D., Ženíšková K., Šubrtová K., Mach J., Štursa J., Machado M., Zahedifard F. et al. (2022). Repurposing of Mitotam: Novel anti-cancer drug candidate exhibits potent activity against major protozoan and fungal pathogens. ASM Journals – Antimicrobial agents and chemotherapy, vol. 66(8).

Horáčková V., Voleman L, Hagen K. D., Petrů M., Vinopalová M., Weisz F., Janowicz N., Marková L., Motyčková A., Najdrová V., Tůmová P., Dawson S. C., Doležal P. (2022). Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis. Open Biol.12210361210361

Fry M. Y., Najdrová V., Maggiolo A. O. et al. (2022) Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3. Nat Struct Mol Biol 29, 820–830.

Kornalíková M., Hampl V., Treitli S. C. (2022) Investigation of the genome sizes and ploidy within the genus Monocercomonoides. The Journal of eukaryotic mikrobiology, vol. 69, 4.

Willen L., Milton P., Hamley J. I. D., Walker M., Osei-Atweneboana M. Y., Volf P., et al. (2022) Demographic patterns of human antibody levels to Simulium damnosum s.l. saliva in onchocerciasis-endemic areas: An indicator of exposure to vector bites. PLoS Negl Trop Dis 16(1): e0010108.

Ticha L., Sadlova J., Bates P. et al. (2022) Experimental infections of sand flies and geckos with Leishmania (Sauroleishmania) adleri and Leishmania (S.) hoogstraali. Parasites Vectors 15, 289.

Sádlová J., Bacíková D., Bečvář T., Vojtková B., England M., Shaw J., Volf P. (2022) Porcisia transmission by prediuresis of sand flies. Front. Cell. Infect. Microbiol. 12:981071.

Piel L., Rajan K. S., Bussotti G., Varet H., Legendre R., Proux C., et al. (2022) Experimental evolution links post-transcriptional regulation to Leishmania fitness gain. PLoS Pathog 18(3): e1010375.

de Oliveira Miranda D. E., da Silva Sales K. G., Aguiar Figueredo L., Andrade de Oliveira S., Vasconcelos do Nascimento A., Lira Torres D. J., Sůmová P., Volf P., Brandão-Filho S. P., da Cunha Gonçalves de Albuquerque S., Barros de Lorena V. M., Dantas-Torres F. (2022) Effects of Migonemyia migonei salivary gland homogenates on Leishmania (Viannia) braziliensis infection in BALB/c mice. Acta Tropica, vol 227, 106271.

Linhart P., Bandouchova H., Zukal J., Votýpka J., Baláž V., Heger T., Kalocsanyiova V., Kubickova A., Nemcova M., Sedlackova J., Seidlova V., Veitova L., Vlaschenko A., Divinova  R., Pikula J. (2022) Blood parasites and health status of hibernating and non-hibernating Noctule bats (Nyctalus noctula). Microorganisms. 10, 1028

Mendoza-Roldan J. A., Votýpka J., Bandi C., Epis S., Modrý D., Tichá L., Volf P., Otranto D. (2022) Leishmania tarentolae: A new frontier in the epidemiology and control of the leishmaniases. Transboundary and emerging diseases.

Gerasimov E. S., Ramirez-Barrios R., Yurchenko V., Zimmer S. L. (2022) Trypanosoma cruzi strain and starvation-driven mitochondrial RNA editing and transcriptome variability. RNA, 993-1012. doi: 10.1261/rna.079088.121.

Kostygov A. Y., Frolov A. O., Malysheva M. N. et al. (2022) Development of two species of the Trypanosoma theileri complex in tabanids. Parasites Vectors 15, 95.

Chmelová Ľ., Bianchi C., Albanaz A. T. S., Režnarová J., Wheeler R., Kostygov A. Y., Kraeva N., Yurchenko V. (2022) Comparative analysis of three trypanosomatid catalases of different origin. Antioxidants, 11, 46.

Vargová R., Hanousková P., Salamonová J., Žihala D., Silberman J. D., Eliáš M., Čepička I. (2022) Evidence for an independent hydrogenosome-to-mitosome transition in the CL3 lineage of fornicates. Front. Microbiol. 13:866459. doi: 10.3389/fmicb.2022.866459

Dewees S. I., Vargová R., Hardin K. R., Turn R. E., Devi S., Linnert J., Wolfrum U., Caspary T., Eliáš M., Kahn R. A. (2022) Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E. Molecular Biology of the Cell 33, 4.

Pánek T., Barcyté D., Treitli S. C. et al. (2022) A new lineage of non-photosynthetic green algae with extreme organellar genomes. BMC Biol 20, 66.

Oborník M. (2022) Organellar evolution: A path from benefit to dependence. Microorganisms 10, 122

Perner J., Kucera M., Frantova H., Urbanova V., Kopacek P., Sima R. (2022) Lyme disease transmission by severely impaired ticks. Open Biol. 2022 Feb;12(2):210244.

Kořený L., Oborník M., Horáková E., Waller R. F., Lukeš J. The convoluted history of haem biosynthesis. Biol Rev Camb Philos Soc. 2022;97(1):141-162. doi:10.1111/brv.12794

Macháček T., Leontovyč R., Šmídová B., Majer M., Vondráček O., Vojtěchová I., Petrásek T., Horák P. (2022): Mechanisms of the host immune response and helminth-induced pathology during Trichobilharzia regenti (Schistosomatidae) neuroinvasion in mice. PLoS Pathogens 18: e1010302. DOI: 10.1371/journal.ppat.1010302 [IF2020=6.823]

Malych R., Stopka P., Mach J., Kotabová E., Prášil O., Sutak R. Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae. Comput Struct Biotechnol J. 2022

Narayanasamy R. K., Rada P., Zdrha A., Ranst M., Neyts J. & Tachezy J. (2022). Cytidine nucleoside analog is an effective antiviral drug against Trichomonasvirus. Journal of Microbiology, Immunology and infection: Volume 55, Issue 2, pages 191-198.


Verner Z., Žárský V., Le T., Narayanasamy R. K., Rada P., Rozbeský D., et al. (2021) Anaerobic peroxisomes in Entamoeba histolytica metabolize myo-inositol. PLoS Pathog 17(11): e1010041.

Tortorelli G., Pettollino F., Lai De-Hua, Tomčala A., Bacic A., Oborník M., Lukeš J., McFadden G. I. (2021). The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia. Journal of Phycology.

Petrů M., Dohnálek V., Füssy Z., Doležal P. Fates of sec, tat, and YidC translocases in mitochondria and other eukaryotic compartments. Molecular Biology and Evolution, Volume 38, Issue 12. December 2021. Pages 5241–5254.

Blanvillain C., Saavedra S., Withers T., Votýpka J., Laroucau K., Lowenski S., Modrý D. (2021) Screening of diseases in wild exotic birds on Tahiti Island – implications for French Polynesian conservation. Pacific Conservation Biology 27, 284-295.

Tůmová P., Voleman L., Klingl A. et al. Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle. BMC Biol 19, 193 (2021).

Poláková E., Záhonová K., Albanaz A., Butenko A., Lukeš J., & Yurchenko V. (2021). Diverse telomeres in trypanosomatids. Parasitology, 148(10), 1254-1270. doi:10.1017/S0031182021000378

Kalčic F., Frydrych J., Doleželová E., Slapničková M., Pachl P., Poštová-Slavětínská L., Dračínský M., Hocková D., Zíková A., Janeba Z. (2021). C1´-Branched acyclic nucleoside phosphonates mimicking adenosine monophosphate: Potent inhibitors of Trypanosoma brucei adenine phosphoribosyltransferase. European Journal of Medicinal Chemistry: Volume 225, 113798

Perner J., Hatalova T., Cabello-Donayre M., Urbanova V., Sojka D., Frantova H., Hartmann D., Jirsova D., Pérez-Victoria J. M. & Kopacek P. (2021). Haem-responsive gene transporter enables mobilization of host haem in ticks. Open Biology: Volume 11, Issue 9.

Kotál J., Polderdijk S. G. I., Langhansová H., Ederová M., Martins L. A., Beránková Z., Chlastáková A., Hajdušek O., Kotsyfakis M., Huntington J. A., Chmelař J. Ixodes ricinus salivary serpin Iripin-8 inhibits the intrinsic pathway of coagulation and complement. International Journal of Molecular Sciences. 2021; 22(17):9480.

Paris Z. (2021). Nuclear tRNA export in trypanosomes: A journey full of twists and turns guided by tRNA modifications. Parasitology, 148(10), 1219-1222. doi:10.1017/S0031182021000482

Kostygov A. Y., Grybchuk D., Kleschenko Y., Chistyakov D. S., Lukashev A. N., Gerasimov E. S., Yurchenko V. Analyses of Leishmania-LRV co-phylogenetic patterns and evolutionary variability of viral proteins. Viruses. 2021; 13(11):2305.

Poláková E., Albanaz A. T. S., Zakharova A., Novozhilova T. S., Gerasimov E. S., Yurchenko V. (2021) Ku80 is involved in telomere maintenance but dispensable for genomic stability in Leishmania mexicana. PLoS Negl Trop Dis 15(12): e0010041.

Zakharova A., Saura A., Butenko A., Podešvová L., Warmusová S., Kostygov A. Y., Nenarokova A., Lukeš J., Opperdoes F. R., Yurchenko V. (2021). A new model trypanosomatid, Novymonas esmeraldas: Genomic perception of its “Candidatus Pandoraea novymonadis“ endosymbiont. Eukaryotic Cells,

Vargová R., Wideman J. G., Derelle R., Klimeš V., Kahn R. A., Dacks J. B., Eliáš M. A eukaryote-wide perspective on the diversity and evolution of the ARF GTPase protein family. Genome Biology and Evolution, Volume 13, Issue 8, August 2021, evab157.

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Sumova P., Sanjoba C., Willen L., Polanska N., Matsumoto Y., Noiri E., Paul S. K., Ozbel Y., Volf P. (2021) PpSP32-like protein as a marker of human exposure to Phlebotomus argentipes in Leishmania donovani foci in Bangladesh. International Journal for Parasitology 51: 1059-

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Husnik F., Tashyreva D., Boscaro V., Geoge E. E., Lukeš J., Keeling P. J. (2021). Bacterial and archaeal symbioses with protists. Current Biology: Volume 31, Issue 13, Pages R862-R877.

Kulkarni S., Rubio M. A. T., Hegedüsová E., Ross R. L., Limbach P. A., Alfonzo J. D., Paris Z. Preferential import of queuosine-modified tRNAs into Trypanosoma brucei mitochondrion is critical for organellar protein synthesis. Nucleic Acids Research, Volume 49, Issue 14, 20 August 2021. Pages 8247–8260,

Paris Z., Svobodová M., Kachale A., Horáková E., Nenarokova A. & Lukeš J. (2021). A mitochondrial cytidine deaminase is responsible for C to U editing of tRNATrp to decode the UGA codon in Trypanosoma brucei. RNA Biology, 18:sup1, 278-286. DOI: 10.1080/15476286.2021.1940445

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Smilansky V., Jirků M., Milner D. S., Ibáñez R., Gratwicke B., Nicholls A., Lukeš J., Chambouvet A. & Richards T. A. (2021). Expanded host and geographic range of tadpole associations with the Severe Perkinsea infection group. Volume 17, Issue 6.

Mishra A., Naseer Kaur J., McSkimming D. I., Hegedüsová E., Dubey A. P., Ciganda M., Paris Z., Read L. K. (2021). Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei.

Richtová J., Sheiner L., Gruber A., Yang S. M., Kořený L., Striepen B., Oborník M. Using diatom and apicomplexan models to study the heme pathway of Chromera velia. International Journal of Molecular Sciences. 2021; 22(12):6495.

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Sojka D., Šnebergerová P., Robbertse L. Protease inhibition—An established strategy to combat infectious diseases. International Journal of Molecular Sciences. 2021; 22(11):5762.

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Lopes A. L. K., Kriegová E., Lukeš J., Krieger M. A., Ludwig A. (2021) Distribution of Merlin in eukaryotes and first report of DNA transposons in kinetoplastid protists. PLoS ONE 16(5): e0251133.

Oborník M. (2021) Enigmatic evolutionary history of porphobilinogen deaminase in eukaryotic phototrophs. Biology 10, no. 5: 386.

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Pyrih J., Pánek T., Durante I. M., Rašková V., Cimrhanzlová K., Kriegová E., Tsaousis A. D., Eliáš M., Lukeš J. Vestiges of the bacterial signal recognition particle-based protein targeting in mitochondria. Molecular Biology and Evolution, Volume 38, Issue 8, August 2021, Pages 3170–3187,

Bílý T., Sheikh S., Mallet A., Bastin P., Pérez-Morga D., Lukeš J., Hashimi H. (2021) Ultrastructural changes of the mitochondrion during the life cycle of Trypanosoma brucei.

Turnšek J., Brunson J. K., del Pilar Martinez Viedma M., Deerinck T. J., Horák A., Oborník M., Bielinski V. A., Allen A. E. (2021) Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway. eLife 10:e52770.

Padilla-Mejia N. E., Koreny L., Holden J., Vancová M., Lukeš J., Zoltner M., Field M. C. A hub-and-spoke nuclear lamina architecture in trypanosomes. J Cell Sci. 2021 Jun 15;134(12):jcs251264. 

Sharma P. P., Kumar S., Srivastava S., Srivastava M., Jee B., Gorobets N. Y., Kumar D., Kumar M., Asthana S., Zhang P., Poonam, Zoltner M., Rathi B. Computational study of novel inhibitory molecule, 1-(4-((2S,3S)-3-amino-2-hydroxy-4-phenylbutyl)piperazin-1-yl)-3-phenylurea, with high potential to competitively block ATP binding to the RNA dependent RNA polymerase of SARS-CoV-2 virus. J Biomol Struct Dyn. 2021 Jun 21:1-19. 

Hammond M., Zoltner M., Garrigan J., Butterfield E., Varga V., Lukeš J., Field M. C. The distinctive flagellar proteome of Euglena gracilis illuminates the complexities of protistan flagella adaptation. New Phytol. 2021 Jul 22.

Vorel J., Cwiklinski K., Roudnický P., Ilgová J., Jedličková L., Dalton J. P., Mikeš L., Gelnar M., Kašný M. (2021): Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling. BMC Genomics 22: 274. DOI: 10.1186/s12864-021-07589-z [IF2020=3.969]

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Corrales R. M., Vaselek S., Neish R., Berry L., Brunet C. D., Crobu L., Kuk N., Mateos-Langerak J., Robinson D. R., Volf P., Mottram J. C., Sterkers Y., Bastien P. (2021) The kinesin of the flagellum attachment zone in Leishmania is required for cell morphogenesis, cell division and virulence in the mammalian host. PLoS Pathogens 17: e1009666.

Telleria E. L., Tinoco-Nunes B., Lestinova T., De Avellar L. M., Tempone A. J., Pitaluga A. N., Volf P., Traub-Cseko Y. M.  (2021) Lutzomyia longipalpis antimicrobial peptides: Differential expression during development and potential involvement in vector interaction with Microbiota and Leishmania. MICROORGANISMS 9: Article Number 1271.

Willen L., Basanez M. G., Dvorak V., Veriegh F. B. D., Aboagye F. T., Idun B., Osman M. E., Osei-Atweneboana M. Y., Courteney O., Volf P. (2021). Human immune response against salivary antigens of Simulium damnosus s.l.: A new epidemiological marker for exposure to blackfly bites in onchocerciasis endemic areas. PLoS Neglected Tropical Diseases 15: e0009512.

Kniha E., Dvorak V., Milchram M., Obwaller A. G., Kohsler M., Poeppl W., Antoniou M., Chaskopoulou A., Paronyan L., Stefanovski J., Mooseder G., Volf P., Walochnik J. (2021) Phlebotomus (Adlerius) simici NITZULESCU, 1931: first record in Austria and phylogenetic relationship with other Adlerius species. PARASITES & VECTORS 14: Article Number 20.

Kniha E., Milchram M., Dvorak V., Halada P., Obwaller A. G., Poeppl W., Mooseder G., Volf P., Walochnik J. (2021). Ecology, seasonality and host preferences of Austrian Phlebotomus (Transphlebotomus) mascittii Grassi, 1908, populations. PARASITES & VECTORS 14: Article Number 291.

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Becvar T., Vojtkova B., Siriyasatien P., Votypka J. Modry D., Jahn P., Bates P., Carpenter S., Volf P. & Sadlova J. (2021) Experimental transmission of Leishmania (Mundinia) parasites by biting midges (Diptera: Ceratopogonidae). PLoS Pathogens 17(6): e1009654.

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Votýpka J., Petrželková K. J., Brzoňová J., Jirků M., Modrý D. & Lukeš J. (2021) How monoxenous trypanosomatids revealed hidden feeding habits of their tsetse fly hosts. Folia Parasitologica 68: 019.

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Charyyeva A., Çetinkaya Ü., Özkan B., Şahin S., Yaprak N., Şahin I., Yurchenko V., Kostygov A. Y. Genetic diversity of Leishmania tropica: unexpectedly complex distribution pattern. Acta Trop., 2021, 218: 105888.

Albanaz A. T. S., Gerasimov E. S., Shaw J. J., Sádlová J., Lukeš J., Volf P., Opperdoes F. R., Kostygov A. Y., Butenko A., Yurchenko V. Genome analysis of Endotrypanum and Porcisia spp., closest phylogenetic relatives of Leishmania, highlights the role of amastins in shaping pathogenicity. Genes. 2021, 12(3): 444.

Gerasimov E. S., Gasparyan A. A., Afonin D. A., Zimmer S. L., Kraeva N., Lukeš J., Yurchenko V., Kolesnikov A. Complete minicircle genome of Leptomonas pyrrhocoris reveals a source of its non-canonical mitochondrial RNA editing events. Nucl. Acids Res., 2021, 49(6): 3354-3370.

Frolov A. O., Kostygov A. Y., Yurchenko V. Development of monoxenous trypanosomatids and phytomonads in insects. Trends Parasitol., 2021, 37(6): 538-551.

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Konorov E. A., Yurchenko V., Patraman I. V., Lukashev A. N., Oyun N. Y. The effects of genetic drift and genomic selection on differentiation and local adaptation of the introduced populations of Aedes albopictus in southern Russia. Peer J., 2021, 9: e11776.

Barcytė D., Eikrem W., Engesmo A., Seoane S., Wohlmann J., Horák A., Yurchenko T., Eliáš M. Olisthodiscus represents a new class of Ochrophyta. J. Phycol., 2021, 57: 1094-1118.

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Larue G. E., Eliáš M., Roy S. W. Expansion and transformation of the minor spliceosomal system in the slime mold Physarum polycephalum. Curr. Biol., 2021, 31: P3125-3131.E4.

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Kostygov A.Y., Karnkowska A., Votýpka J., Tashyreva D., Maciszewski K., Yurchenko V. & Lukeš J. (2021) Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses. Open Biology 11: 200407.

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Procházková M., Füzik T., Grybchuk D., Luca Falginell F., Podešvová L., Yurchenko V., Vácha R., Plevka P. Capsid structure of Leishmania RNA Virus 1. Journal of Virology. Jan 2021, 95 (3) e01957-20, doi:10.1128/JVI.01957-20

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Opperdoes F. R., Butenko A., Zakharova A., Gerasimov E. S., Zimmer S. L., Lukeš J., & Yurchenko V. (2021). The remarkable metabolism of Vickermania ingenoplastis: Genomic predictions. Pathogens, 10(1), 68.

Butenko A., Hammond M., Field M. C., Ginger M. L., Yurchenko V., & Lukeš J. (2021). Reductionist pathways for parasitism in Euglenozoans? Expanded datasets provide new insights. Trends in parasitology, 37(2), 100–116.

Mahmood S., Sima R., Urbanova V., Trentelman J. J. A., Krezdorn N., Winter P., Kopacek P., Hovius J. W., Hajdusek O. (2021). Identification of tick Ixodes ricinus midgut genes differentially expressed during the transmission of Borrelia afzelii spirochetes using a transcriptomic approach. Front Immunol. 11:612412. doi: 10.3389/fimmu.2020.612412.

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Grechnikova M., Ženíšková K., Malych R., Mach J., Sutak R. Copper detoxification machinery of the brain-eating amoeba Naegleria fowleri involves copper-translocating ATPase and the antioxidant system. Int J Parasitol Drugs Drug Resist. 2020

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Maia C., Cristovao J., Pereira A., Kostalova T., Lestinova T., Sumova P., Volf P., Campino L. (2020) Monitoring Leishmania infection and exposure to Phlebotomus perniciosus using minimal and non-invasive canine samples. PARASITES & VECTORS 13: DOI: 10.1186/s13071-020-3993-7

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Tomčala A., Michálek J., Schneedorferová I., Füssy Z., Gruber A., Vancová M., Oborník M. Fatty acid biosynthesis in chromerids. Biomolecules 2020, 10, 1102.

Thomas S. Corrigan, Leilani M. Lotti Diaz, Sarah E. Border, Steven C. Ratigan, Kayla Q. Kasper, Daniel Sojka, Pavla Fajtova, Conor R. Caffrey, Guy S. Salvesen, Craig A. McElroy, Christopher M. Hadad & Özlem Doğan Ekici (2020) Design, synthesis, and in vitro evaluation of aza-peptide aldehydes and ketones as novel and selective protease inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry. 35:1, 1387-1402, DOI: 10.1080/14756366.2020.1781107

Pyrih J., Rašková V., Škodová-Sveráková I., Pánek T., Lukeš J. (2020) ZapE/Afg1 interacts with Oxa1 and its depletion causes a multifaceted phenotype. PLoS ONE 15(6): e0234918.

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Speijer D. Hammond M., Lukeš J. (2020) Comparing early eukaryotic integration of mitochondria and chloroplasts in the light of internal ROS challenges: Timing is of the essence. mBio, 11 (3) e00955-20; DOI: 10.1128/mBio.00955-20

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Michael J. Hammond, Anna Nenarokova, Anzhelika Butenko, Martin Zoltner, Eva Lacová Dobáková, Mark C. Field, Julius Lukeš. A uniquely complex mitochondrial proteome from Euglena gracilis. Molecular Biology and Evolution, Volume 37, Issue 8, August 2020, Pages 2173–2191,

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Jalovecka M., Hajdusek O., Sojka D., Kopacek P. and Malandrin L. (2018) The complexity of piroplasms life cycles. Front. Cell. Infect. Microbiol. 8:248. DOI: 10.3389/fcimb.2018.00248

Peña-Diaz P., Mach J., Kriegová E., Poliak P., Tachezy J., Lukeš J. (2018) Trypanosomal mitochondrial intermediate peptidase does not behave as a classical mitochondrial processing peptidase. PLoS ONE 13(4): e0196474.

Peña-Diaz P., Lukeš J. Fe–S cluster assembly in the supergroup Excavata. J Biol Inorg Chem 23, 521–541 (2018).

Harmer J., Yurchenko V., Nenarokova A., Lukeš J., & Ginger M. (2018). Farming, slaving and enslavement: Histories of endosymbioses during kinetoplastid evolution. Parasitology, 145(10), 1311-1323. DOI:10.1017/S0031182018000781

Marszewska A., Cichy A., Bulantová J., Horák P., Żbikowska E. 2018. Potamopyrgus antipodarum as a potential defender against swimmer’s itch in European recreational water bodies—experimental study. PeerJ 6:e5045

Vivas Muñoz J. C., Hilt S., Horák P. et al. Impact of trematode infections on periphyton grazing rates of freshwater snails. Parasitol Res 117, 3547–3555 (2018).

Willen L., Mertens P., Volf P. (2018) Evaluation of the rSP03B sero-strip, a newly proposed rapid test for canine exposure to Phlebotomus perniciosus, vector of Leishmania infantum. PLoS Negl Trop Dis 12(8): e0006607.

Sadlova J., Homola M., Myskova J., Jancarova M., Volf P. (2018) Refractoriness of Sergentomyia schwetzi to Leishmania spp. is mediated by the peritrophic matrix. PLoS Negl Trop Dis 12(4): e0006382.

Halada P., Hlaváčková K., Risueño J., Berriatua E., Volf P., Dvořák V. Effect of trapping method on species identification of phlebotomine sandflies by MALDI-TOF MS protein profiling: Trap effect on sandfly protein profiling. Medical and Veterinary Entomology. 2018, 32. DOI: 10.1111/mve.12305.

Spodareva V. V., Grybchuk-Ieremenko A., Losev A., Votýpka J., Lukeš J., Yurchenko V., Kostygov A. Y. Diversity and evolution of anuran trypanosomes: insights from the study of European species. Parasites & Vectors. 2018, 11(1), 447. DOI: 10.1186/s13071-018-3023-1.

Treitli, S. C., Kotyk, M., Yubuki, N., Jirounková, E., Vlásáková, J., Smejkalová, P., Šípek, P., Čepička, I., Hampl, V. Molecular and morphological diversity of the oxymonad genera Monocercomonoides and Blattamonas gen. nov. Protist. 2018, 169(5), 744-783. DOI: 10.1016/j.protis.2018.06.005.

Vacek, V., Novák, L. V. F., Treitli, S. C., Táborský, P., Čepička, I., Kolísko, M., Keeling, P. J., Hampl, V. Fe–S Cluster assembly in oxymonads and related protists. Molecular Biology and Evolution. 2018, 35(11), 2712–2718. DOI: 10.1093/molbev/msy168.

Mach J., Bílá J., Ženíšková K., Arbon D., Malych R., Glavanakovová M., Nývltová E., Sutak R. Iron economy in Naegleria gruberi reflects its metabolic flexibility. International Journal for Parasitology. 2018, 48(9-10), 719-727. DOI: 10.1016/j.ijpara.2018.03.005.

Schoener E., Uebleis S. S., Cuk C., Nawratil M., Obwaller A. G., Zechmeister T., et al. (2018) Trypanosomatid parasites in Austrian mosquitoes. PLoS ONE 13(4): e0196052.

Silva F., Kostygov A., Spodareva V., Butenko A., Tossou R., Lukeš J., Yurchenko V., Alves J. (2018). The reduced genome of Candidatus Kinetoplastibacterium sorsogonicusi, the endosymbiont of Kentomonas sorsogonicus (Trypanosomatidae): Loss of the haem-synthesis pathway. Parasitology, 145(10), 1287-1293. DOI:10.1017/S003118201800046X.

Záhonová K., Petrželková R., Valach M. et al. Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase. Sci Rep 8, 5239 (2018).

Hackenberg M., Kotsyfakis M. Exosome-mediated pathogen transmission by arthropod vectors. Trends in Parasitology. 2018, 34(7), 549-552. DOI: 10.1016/

Perner J., Kropáčková S., Kopáček P., Ribeiro J. M. C. (2018) Sialome diversity of ticks revealed by RNAseq of single tick salivary glands. PLoS Negl Trop Dis 12(4): e0006410.

Heiss A. A., Kolísko M., Ekelund F., Brown M. W., Roger A. J., Simpson A. G. B. Combined morphological and phylogenomic re-examination of malawimonads, a critical taxon for inferring the evolutionary history of eukaryotes. Royal society open science. 2018, 5(4). DOI: 10.1098/rsos.171707. ISSN 2054-5703.

Lukeš J., Butenko A., Hashimi H., Maslov D. A., Votýpka J., Yurchenko V. Trypanosomatids are much more than just trypanosomes: Clues from the expanded family tree. Trends in Parasitology. 2018, 34(6), 466-480. DOI: 10.1016/

Procházková M., Panicucci B. & Zíková A. Cultured bloodstream Trypanosoma brucei adapt to life without mitochondrial translation release factor 1. Sci Rep 8, 5135 (2018).

Perner J., Kotál J., Hatalová T., Urbanová V., Bartošová-Sojková P., Brophy P. M., Kopáček P. Inducible glutathione S-transferase (IrGST1) from the tick Ixodes ricinus is a haem-binding protein. Insect Biochemistry and Molecular Biology. 2018, 95, 44-54. DOI: 10.1016/j.ibmb.2018.02.002.

Tashyreva D., Prokopchuk G., Votýpka J., Yabuki A., Horák A., Lukeš J. Life cycle, ultrastructure, and phylogeny of new diplonemids and their endosymbiotic bacteria. MBio. 2018, 9(2). DOI: 10.1128/mBio.02447-17.

Lynn, D. H., Kolísko, M., Bourland, W. Phylogenomic analysis of Nassula variabilis n. sp., Furgasonia blochmanni, and Pseudomicrothorax dubius confirms a Nassophorean clade. Protist. 2018, 169(2), 180-189. DOI: 10.1016/j.protis.2018.02.002.


prof. RNDr. Jan Tachezy, Ph.D. prof. RNDr. Jan Tachezy, Ph.D.

prof. RNDr.
Jan Tachezy, Ph.D.

Head of Cellular Biology and Virology Research Programme, Faculty of Science, Charles University
Mgr. Pavel Doležal, Ph.D. Mgr. Pavel Doležal, Ph.D.

Pavel Doležal, Ph.D.

Head of Group Mechanisms for Transport of Proteins through Mitochondrial and Bacterial Membranes
+420325873945, +420325873909
doc. Mgr. Vladimír Hampl, Ph.D. doc. Mgr. Vladimír Hampl, Ph.D.

doc. Mgr.
Vladimír Hampl, Ph.D.

Head of Group Genomics of Eukaryotes and Lateral Gene Transfer
+420325873911, +420325873952
Doc. RNDr. Ivan Hrdý, Ph.D. Doc. RNDr. Ivan Hrdý, Ph.D.

Doc. RNDr.
Ivan Hrdý, Ph.D.

Head of Department of Parasitology of the Faculty of Science CUNI
Ravi Kumar Narayanasamy

Ravi Kumar Narayanasamy

Postdoctoral researcher
Mgr. Petr Rada, Ph.D. Mgr. Petr Rada, Ph.D.

Petr Rada, Ph.D.

Assistant Professor
Mgr. Tamara Smutná, Ph.D. Mgr. Tamara Smutná, Ph.D.

Tamara Smutná, Ph.D.

Postdoctoral researcher
RNDr. Róbert Šuťák, Ph.D. RNDr. Róbert Šuťák, Ph.D.

Róbert Šuťák, Ph.D.

Head of Group Uptake and Intracellular Metabolism of Metals
+420325873910, +420325873944
RNDr. Zdeněk Verner, Ph.D. RNDr. Zdeněk Verner, Ph.D.

Zdeněk Verner, Ph.D.

Postdoctoral researcher


In the frame of this project, three strategic foreign partners will be included, which significantly contribute to fulfilment of the research aims, increase the qualification of our research team members and extend the international.