Stem cells in epidermis and their use in tissue engineering

Prof. MUDr. Karel Smetana, DrSc. — Project head

1st Faculty of Medicine of the Charles University

About us

The project is aimed at studying possibilities of epidermal stem cell isolation. The epidermal stem cells and the multipotent neural crest originated stem cells represent the most easily accessible stem cells in human body. The in vitro propagation and controlled differentiation will be investigated too.

Our interest is focused predominantly on study of the microenvironment of these cells under physiological and pathological conditions (cancer) that is necessary for their expansion in vitro.

Research Objectives:

Development of separation and cultivation methods for the preparation of stem cells from the human epidermis
Methods of the characterization and differentiation of the obtained cell types
Study of the influence of the microenvironment on the stem cells function and their differentiation
Development of the new clinical applications

More info at: Skin stem cell lab

Further information can be found at www.phenogenomics.cz or at the link https://biocev.lf1.cuni.cz/

News

The research centre the faculty deserved

From Chemistry to Medicine (and Back)

Oestrogen receptor modulators protect women against covid and other infections

Scientists from the IEM CAS and BIOCEV organized a one-day regenerative medicine workshop BIOCEV Regeneration I

Interview with prof. Karel Smetana abut the new project Center of Tumor Ecology

Czech TV: New Center of Tumor Ecology

Publications

2019

Vsiansky V, Svobodova M, Gumulec J, Cernei N, Sterbova D, Zitka O, Kostrica R, Smilek P, Plzak J, Betka J, Kalfert D, Masarik M, Raudenska M. Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers. Cells. 2019; 8(5):428. (IF 4.829)

Münster L, Fojtů M, Capáková Z, Vaculovič T, Tvrdoňová M, Kuřitka I, Masařík M, Vícha J. Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs. Biomacromolecules. 2019 Apr 8;20(4):1623-1634. doi: 10.1021/acs.biomac.8b01807. (IF 5.738)

Plzák J, Bouček J, Bandúrová V, Kolář M, Hradilová M, Szabo P, Lacina L, Chovanec M, Smetana K Jr. The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy. Cancers (Basel). 2019 Mar 28;11(4). pii: E440. doi: 10.3390/cancers11040440. (IF 5.326)

Lacina L, Čoma M, Dvořánková B, Kodet O, Melegová N, Gál P, Smetana K Jr. Evolution of Cancer Progression in the Context of Darwinism. Anticancer Res. 2019 Jan;39(1):1-16. doi: 10.21873/anticanres.13074. (IF 1.865)

Peltanova B, Raudenska M, Masarik M. Effect of tumor microenvironment on pathogenesis of the head and neck squamous cell carcinoma: a systematic review. Mol Cancer. 2019 Mar 30;18(1):63. doi: 10.1186/s12943-019-0983-5. (IF 7.78)

Lacina L, Brábek J, Král V, Kodet O, Smetana K Jr. Interleukin-6: a molecule with complex biological impact in cancer. Histol Histopathol. 2019 Feb;34(2):125-136. doi: 10.14670/HH-18-033. (IF 2.015)

Raudenska M, Balvan J, Fojtu M, Gumulec J, Masarik M. Unexpected therapeutic effects of cisplatin. Metallomics. 2019 Jul 17;11(7):1182-1199. doi: 10.1039/c9mt00049f.

2018

Solís D, Bovin NV, Davis AP, Jiménez-Barbero J, Romero A, Roy R, Smetana K Jr, Gabius HJ. A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code. Biochim Biophys Acta. 2015 Jan;1850(1):186-235.

Lacina L, Kodet O, Dvořánková B, Szabo P, Smetana K Jr.: Ecology of melanoma cell. Histol Histopathol 33: 247-254 (2018) (IF 2.025)

Kodet O., Dvořánková B., Bendlová B., Sýkorová V., Krajsová I., Štork J, Kučera J., Szabo P., Strnad H., Kolář M., Vlček Č., Smetana K. Jr., Lacina L.: Microenvironment‑driven resistance to B ‑Raf inhibition in a melanoma patient is accompanied by broad changes of gene methylation and expression in distal fibroblasts. Int J Mol Med 41: 3687-2703 (2018). (IF 2.341)

2017

Gál P, Varinská L, Fáber L, Novák Š, Szabo P, Mitrengová P, Mirossay A, Mučaji P, Smetana K. Jr.: How signaling molecules regulate tumor microenvironment: Parallels to wound Repair. Molecules. 22: pii: E1818 (2017) (IF 2.861)

2016

Smetana K., jr. Lacina L., Szabo, P., Dvořánková, B., Brož, P., Šedo, A.: Ageing as an important risk factor for cancer. Anticancer Res 36: 5009-5018 (2016) (IF 1.895)

2015

Kodet, O., Lacina, L., Krejčí, E., Dvořánková, B., Grim, M., Štork, J., Kodetová, D., Vlček, Č., Šáchová, J., Kolář, M., Strnad, H., Smetana, K.: Melanoma cells influence the differentiation pattern of human epidermal keratinocytes. Mol Cancer. doi: 10.1186/1476-4598-14-1 (2015)

Smetana K Jr, Szabo P, Gal P, André S, Gabius HJ, Kodet O, Dvořánková B. Emerging role of tissue lectins as microenvironmental effectors in tumors and wounds. Histol Histopathol. 2015 Mar;30(3):293-309.

Lacina, L. Plzak J., Kodet O., Szabo P., Chovanec, M., Dvorankova B., Smetana, K. Jr.: Cancer microenvironment: What can we learn from the stem cell niche. Int J Mol Sci 16: 24094-24110 (2015) (IF 2. 862)

2013

Smetana, K, Jr et al: Role of stromal fibroblasts in cancer originated from squamous epithelia. In Bai, X. (ed) Dermal Fibroblasts: Histological Perspectives, Characterization and Role in Disease. Nova Sciences Publishers, New York (2013) pp. 83-94

2012

Kolář M et al.: Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro, immunohistochemical and transcriptomic analyses. Biol Cell 104: 738-751 (2012)