OMICS Proteomics

OMICS Proteomics

Mgr. Karel Harant

Mgr. Karel Harant — Laboratory head

About us

We offer complex proteome analysis and quantification on the latest generation of Mass spectrometry equipment–Thermo Orbitrap Fusion. We are able to quantify your sample on the whole proteome level using an untargeted approach or just a selected protein with a targeted analysis. The most common applications are: quantitative analysis of whole proteomes comparing, for example, treated and control samples, analysis of immunoprecipitated proteins and targeted quantification of selected peptides.

Our Services:

  • Preparation of samples for analysis (we are able to prepare a sample for analysis from your tissue or cell line)
  • Proteomic analysis of your samples – identification and quantification of proteins (aproximately 4500 quantified proteins from the human cell line in a single injection)
  • Data analysis and basic statistical evaluation
  • Consultation about experimental designs and how to obtain the maximum amount of informationfrom your experiment
     

CF SERVICES/SUCCESSFUL PROJECT

We are able to quantify your sample using different approaches – label free quantification, SILAC, isobaric tag quantification (iTRAQ), dimethyl labelling etc. We can also offer detection and quantification of various posttranslational modifications (PTMs), such as phosphorylation, acetylation or other PTMs. In addition to basic data analysis which is free for each sample, we can  perform more complex bioinformatic and statistical analysis or train your staff how to use proteomics software and how to interpret proteomic data.

POTENTIAL FOR COOPERATION:

We will be your partner in your research efforts. Each analysis is consulted individually beforehand to perfectly meet your demands. All our services are available for everybody from the academic and non academic areas. We work in pay per analysis mode and we don’t demand co-authorship on your publications. In case of stable and long-term cooperation You can obtain an individualized price quote.

News

Equipment

Proteomic core facility

vacuum concentrator

vacuum concentrator

vacuum concentrator  without cooling , oil rotary vacuum pump

Software

Compound Discoverer 2.0

Compound Discoverer 2.0

http://planetorbitrap.com/compound-discoverer#.XFwbKlVKgUE

TraceFinder

TraceFinder

Software package for targeted SRM and MRM data acquisition, data processing and quantification

https://www.thermofisher.com/order/catalog/product/OPTON-30491

Software - Freeware

Publications

2022

Ronald Malych, Pavel Stopka, Jan Mach, Eva Kotabová, Ondřej Prášil, Robert Sutak, Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae, Computational and Structural Biotechnology Journal, Volume 20, 2022, Pages 287-295, https://doi.org/10.1016/j.csbj.2021.12.023.

Zimmann N, Rada P, Žárský V, Smutná T, Záhonová K, Dacks J, Harant K, Hrdý I, Tachezy J. Proteomic Analysis of Trichomonas vaginalis Phagolysosome, Lysosomal Targeting, and Unconventional Secretion of Cysteine Peptidases. Mol Cell Proteomics. 2022 Jan;21(1):100174. doi: 10.1016/j.mcpro.2021.100174. Epub 2021 Nov 8. PMID: 34763061; PMCID: PMC8717582.

Vondráček O, Mikeš L, Talacko P, Leontovyč R, Bulantová J, Horák P. Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion. Int J Parasitol. 2022 Feb 23:S0020-7519(22)00017-0. doi: 10.1016/j.ijpara.2021.12.003. Epub ahead of print. PMID: 35218763.

Erban T, Klimov PB, Harant K, Talacko P, Nesvorna M, Hubert J. Label-free proteomic analysis reveals differentially expressed Wolbachia proteins in Tyrophagus putrescentiae: Mite allergens and markers reflecting population-related proteome differences. J Proteomics. 2021 Oct 30;249:104356. doi: 10.1016/j.jprot.2021.104356. Epub 2021 Aug 23. PMID: 34438106.

Vacurova E, Trnovska J, Svoboda P, Skop V, Novosadova V, Reguera DP, Petrezselyová S, Piavaux B, Endaya B, Spoutil F, Zudova D, Stursa J, Melcova M, Bielcikova Z, Werner L, Prochazka J, Sedlacek R, Huttl M, Hubackova SS, Haluzik M, Neuzil J. Mitochondrially targeted tamoxifen alleviates markers of obesity and type 2 diabetes mellitus in mice. Nat Commun. 2022 Apr 6;13(1):1866. doi: 10.1038/s41467-022-29486-z. PMID: 35387987.

Smutná T, Dohnálková A, Sutak R, Narayanasamy RK, Tachezy J, Hrdý I. A cytosolic ferredoxin-independent hydrogenase possibly mediates hydrogen uptake in Trichomonas vaginalis. Curr Biol. 2022 Jan 10;32(1):124-135.e5. doi: 10.1016/j.cub.2021.10.050. Epub 2021 Nov 10. PMID: 34762819.

2021

Dienstbier A, Amman F, Petráčková D, Štipl D, Čapek J, Zavadilová J, Fabiánová K, Držmíšek J, Kumar D, Wildung M, Pouchnik D, Večerek B. Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution. Emerg Infect Dis. 2021 Jan;27(1):57-68. doi: 10.3201/eid2701.191541. PMID: 33350934; PMCID: PMC7774529.

Drzmisek, J.; Stipl, D.; Petrackova, D.; Vecerek, B.; Dienstbier, A. Omics Analysis of Blood-Responsive Regulon in Bordetella pertussis Identifies a Novel Essential T3SS Substrate. Int. J. Mol. Sci. 2021, 22, 736.

Sztacho M, Šalovská B, Červenka J, Balaban C, Hoboth P, Hozák P. Limited Proteolysis-Coupled Mass Spectrometry Identifies Phosphatidylinositol 4,5-Bisphosphate Effectors in Human Nuclear Proteome. Cells. 2021 Jan 4;10(1):68. doi: 10.3390/cells10010068. PMID: 33406800; PMCID: PMC7824793.

Čunátová K, Reguera DP, Vrbacký M, Fernández-Vizarra E, Ding S, Fearnley IM, Zeviani M, Houštěk J, Mráček T, Pecina P. Loss of COX4I1 Leads to Combined Respiratory Chain Deficiency and Impaired Mitochondrial Protein Synthesis. Cells. 2021 Feb 10;10(2):369. doi: 10.3390/cells10020369. PMID: 33578848; PMCID: PMC7916595.

Voukali E, Veetil NK, Němec P, Stopka P, Vinkler M. Comparison of plasma and cerebrospinal fluid proteomes identifies gene products guiding adult neurogenesis and neural differentiation in birds. Sci Rep. 2021 Mar 5;11(1):5312. doi: 10.1038/s41598-021-84274-x. PMID: 33674647; PMCID: PMC7935914.

Erban T, Shcherbachenko E, Talacko P, Harant K. A single honey proteome dataset for identifying adulteration by foreign amylases and mining various protein markers natural to honey. J Proteomics. 2021 May 15;239:104157. doi: 10.1016/j.jprot.2021.104157. Epub 2021 Feb 22. PMID: 33631366.

Karel Müller, Petre Ivanov Dobrev, Aleš Pěnčík, Petr Hošek, Zuzana Vondráková, Roberta Filepová, Kateřina Malínská, Federica Brunoni, Lenka Helusová, Tomáš Moravec, Katarzyna Retzer, Karel Harant, Ondřej Novák, Klára Hoyerová, Jan Petrášek, DIOXYGENASE FOR AUXIN OXIDATION 1 catalyzes the oxidation of IAA Amino Acid Conjugates, Plant Physiology, 2021;, kiab242, https://doi.org/10.1093/plphys/kiab242

Lidak T, Baloghova N, Korinek V, Sedlacek R, Balounova J, Kasparek P, Cermak L. CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation. Int J Mol Sci. 2021 May 20;22(10):5394. doi: 10.3390/ijms22105394. PMID: 34065512; PMCID: PMC8161014.

Rouhova L, Kludkiewicz B, Sehadova H, Sery M, Kucerova L, Konik P, Zurovec M. Silk of the common clothes moth, Tineola bisselliella, a cosmopolitan pest belonging to the basal ditrysian moth line. Insect Biochem Mol Biol. 2021 Mar;130:103527. doi: 10.1016/j.ibmb.2021.103527. Epub 2021 Jan 19. PMID: 33476773.

Zabelina V, Takasu Y, Sehadova H, Yonemura N, Nakajima K, Sezutsu H, Sery M, Zurovec M, Sehnal F, Tamura T. Mutation in Bombyx mori fibrohexamerin (P25) gene causes reorganization of rough endoplasmic reticulum in posterior silk gland cells and alters morphology of fibroin secretory globules in the silk gland lumen. Insect Biochem Mol Biol. 2021 Aug;135:103607. doi: 10.1016/j.ibmb.2021.103607. Epub 2021 Jun 5. PMID: 34102294.

Rindos M, Kucerova L, Rouhova L, Sehadova H, Sery M, Hradilova M, Konik P, Zurovec M. Comparison of Silks from Pseudoips prasinana and Bombyx mori Shows Molecular Convergence in Fibroin Heavy Chains but Large Differences in Other Silk Components. Int J Mol Sci. 2021 Jul 31;22(15):8246. doi: 10.3390/ijms22158246. PMID: 34361011; PMCID: PMC8347419.

Makarov M, Meng J, Tretyachenko V, Srb P, Březinová A, Giacobelli VG, Bednárová L, Vondrášek J, Dunker AK, Hlouchová K. Enzyme catalysis prior to aromatic residues: Reverse engineering of a dephospho-CoA kinase. Protein Sci. 2021 May;30(5):1022-1034. doi: 10.1002/pro.4068. Epub 2021 Mar 26. PMID: 33739538; PMCID: PMC8040869.

Ryabchenko B, Soldatova I, Šroller V, Forstová J, Huérfano S. Immune sensing of mouse polyomavirus DNA by p204 and cGAS DNA sensors. FEBS J. 2021 May 10. doi: 10.1111/febs.15962. Epub ahead of print. PMID: 33969628.

Kučera O, Siahaan V, Janda D, Dijkstra SH, Pilátová E, Zatecka E, Diez S, Braun M, Lansky Z. Anillin propels myosin-independent constriction of actin rings. Nat Commun. 2021 Jul 28;12(1):4595. doi: 10.1038/s41467-021-24474-1. PMID: 34321459; PMCID: PMC8319318.

Vanova KH, Pang Y, Krobova L, Kraus M, Nahacka Z, Boukalova S, Pack SD, Zobalova R, Zhu J, Huynh TT, Jochmanova I, Uher O, Hubackova S, Dvorakova S, Garrett TJ, Ghayee HK, Wu X, Schuster B, Knapp PE, Frysak Z, Hartmann I, Nilubol N, Cerny J, Taieb D, Rohlena J, Neuzil J, Yang C, Pacak K. Germline SUCLG2 Variants in Patients with Pheochromocytoma and Paraganglioma. J Natl Cancer Inst. 2021 Aug 20:djab158. doi: 10.1093/jnci/djab158. Epub ahead of print. PMID: 34415331.

Staňková P, Kučera O, Peterová E, Elkalaf M, Rychtrmoc D, Melek J, Podhola M, Zubáňová V, Červinková Z. Western Diet Decreases the Liver Mitochondrial Oxidative Flux of Succinate: Insight from a Murine NAFLD Model. Int J Mol Sci. 2021 Jun 27;22(13):6908. doi: 10.3390/ijms22136908. PMID: 34199098; PMCID: PMC8268937.

Sistilli G, Kalendova V, Cajka T, Irodenko I, Bardova K, Oseeva M, Zacek P, Kroupova P, Horakova O, Lackner K, Gastaldelli A, Kuda O, Kopecky J, Rossmeisl M. Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity. Nutrients. 2021 Jan 29;13(2):437. doi: 10.3390/nu13020437. PMID: 33572810; PMCID: PMC7912192.

Kotabova E, Malych R, Pierella Karlusich JJ, Kazamia E, Eichner M, Mach J, Lesuisse E, Bowler C, Prášil O, Sutak R. Complex Response of the Chlorarachniophyte Bigelowiella natans to Iron Availability. mSystems. 2021 Feb 9;6(1):e00738-20. doi: 10.1128/mSystems.00738-20. PMID: 33563784; PMCID: PMC7883536.

Ujcikova H, Hejnova L, Eckhardt A, Roubalova L, Novotny J, Svoboda P. Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies. Neurochem Int. 2021 Mar;144:104975. doi: 10.1016/j.neuint.2021.104975. Epub 2021 Jan 27. PMID: 33508371.

Zimmann N, Rada P, Žárský V, Smutná T, Záhonová K, Dacks J, Harant K, Hrdý I, Tachezy J. Proteomic analysis of Trichomonas vaginalis phagolysosome, lysosomal targeting, and unconventional secretion of cysteine peptidases. Mol Cell Proteomics. 2021 Nov 8:100174. doi: 10.1016/j.mcpro.2021.100174. Epub ahead of print. PMID: 34763061.

Cermakova K, Demeulemeester J, Lux V, Nedomova M, Goldman SR, Smith EA, Srb P, Hexnerova R, Fabry M, Madlikova M, Horejsi M, De Rijck J, Debyser Z, Adelman K, Hodges HC, Veverka V. A ubiquitous disordered protein interaction module orchestrates transcription elongation. Science. 2021 Nov 26;374(6571):1113-1121. doi: 10.1126/science.abe2913. Epub 2021 Nov 25. PMID: 34822292.

Horváthová L, Žárský V, Pánek T, Derelle R, Pyrih J, Motyčková A, Klápšťová V, Vinopalová M, Marková L, Voleman L, Klimeš V, Petrů M, Vaitová Z, Čepička I, Hryzáková K, Harant K, Gray MW, Chami M, Guilvout I, Francetic O, Franz Lang B, Vlček Č, Tsaousis AD, Eliáš M, Doležal P. Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system. Nat Commun. 2021 May 19;12(1):2947. doi: 10.1038/s41467-021-23046-7. PMID: 34011950; PMCID: PMC8134430.

2020

Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity
Cells. 2020 Feb; 9(2): 443. Published online 2020 Feb 14. doi: 10.3390/cells9020443 PMCID: PMC7072730 PMID: 32075102
David Pajuelo Reguera,1 Kristýna Čunátová,1,2 Marek Vrbacký,1 Alena Pecinová,1 Josef Houštěk,1 Tomáš Mráček,1,* and Petr Pecina1,*

Ujcikova, H., Cechova, K., Jagr, M., Roubalova, L., Vosahlikova, M., & Svoboda, P. (2020). Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days; comparison with animals after 20 days of morphine withdrawal. PLOS ONE, 15(4), e0231721. doi:10.1371/journal.pone.0231721 

Ujcikova, H., Cechova, K., Roubalova, L., Brejchova, J., Kaufman, J., Holan, V., & Svoboda, P. (2020). The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells. Journal of Neuroimmunology, 341, 577191. doi:10.1016/j.jneuroim.2020.577191

Polanska N, Ishemgulova A, Volfova V,
Flegontov P, Votypka J, Yurchenko V, et al. (2020)
Sergentomyia schwetzi: Salivary gland
transcriptome, proteome and enzymatic activities
in two lineages adapted to different blood sources.
PLoS ONE 15(3): e0230537. https://doi.org/
10.1371/journal.pone.0230537

Jankovska E, Lipcseyova D, Svrdlikova M, Pavelcova M, Kubala Havrdova E, Holada K, Petrak J. Quantitative proteomic analysis of cerebrospinal fluid of women newly diagnosed with multiple sclerosis. Int J Neurosci. 2020 Oct 29:1-11. doi: 10.1080/00207454.2020.1837801. Epub ahead of print. PMID: 33059501.

Kocourek F, Stara J, Sopko B, Talacko P, Harant K, Hovorka T, Erban T. Proteogenomic insight into the basis of the insecticide tolerance/resistance of the pollen beetle Brassicogethes (Meligethes) aeneus. J Proteomics. 2020 Dec 27;233:104086. doi: 10.1016/j.jprot.2020.104086. Epub ahead of print. PMID: 33378720.

Čermák V, Gandalovičová A, Merta L, Harant K, Rösel D, Brábek J. High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen. Sci Data. 2020 May 27;7(1):160. doi: 10.1038/s41597-020-0499-2. PMID: 32461585; PMCID: PMC7253430.

Fáberová V, Kalasová I, Krausová A, Hozák P. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells. 2020 May 11;9(5):1191. doi: 10.3390/cells9051191. PMID: 32403279; PMCID: PMC7291030.

Prikryl P, Satrapova V, Frydlova J, Hruskova Z, Zima T, Tesar V, Vokurka M. Mass spectrometry-based proteomic exploration of the small urinary extracellular vesicles in ANCA-associated vasculitis in comparison with total urine. J Proteomics. 2020 Dec 9;233:104067. doi: 10.1016/j.jprot.2020.104067. Epub ahead of print. PMID: 33307252.

Novák J, Jurnečka D, Linhartová I, Holubová J, Staněk O, Štipl D, Dienstbier A, Večerek B, Azevedo N, Provazník J, Beneš V, Šebo P. A Mutation Upstream of the rplN-rpsD Ribosomal Operon Downregulates Bordetella pertussis Virulence Factor Production without Compromising Bacterial Survival within Human Macrophages. mSystems. 2020 Dec 8;5(6):e00612-20. doi: 10.1128/mSystems.00612-20. PMID: 33293402; PMCID: PMC7742992.

Maršíková J., Pavlíčková M., Wilkinson D., Váchová L., Hlaváček O., Hatáková L., Palková Z. (2020) The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in yeast colonies. PNAS first published June 15, 2020 https://doi.org/10.1073/pnas.1922076117

Langerová H, Lubyová B, Zábranský A, et al. Hepatitis B Core Protein Is Post-Translationally Modified through K29-Linked Ubiquitination. Cells. 2020;9(12):2547. Published 2020 Nov 26. doi:10.3390/cells9122547

Korabecna M, Zinkova A, Brynychova I, Chylikova B, Prikryl P, Sedova L, Neuzil P, Seda O. Cell-free DNA in plasma as an essential immune system regulator. Sci Rep. 2020 Oct 15;10(1):17478. doi: 10.1038/s41598-020-74288-2. PMID: 33060738; PMCID: PMC7566599.

Dibus M, Brábek J, Rösel D. A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18. Int J Mol Sci. 2020 Oct 20;21(20):7769. doi: 10.3390/ijms21207769. PMID: 33092266; PMCID: PMC7594087.

2019

Makki A., Rada P, Žárský V., Kereiche S., Kovacik L., Novotny M., Jores T., Rapaport D., Tachezy J. 2019 Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis. PLoS Biology. 17(1):e3000098. doi: 10.1371/journal.pbio.3000098

  

Syslova, E., Landa, P., Stuchlikova, L.R., Matouskova, P., Skalova, L., Szotakova, B., Navratilova, M., Vanek, T. and Podlipna, R. (2019) Metabolism of the anthelmintic drug fenbendazole in Arabidopsis thaliana and its effect on transcriptome and proteome. Chemosphere, 218, 662-669.

Palyzová, A., Zahradník, J., Marešová, H., & Řezanka, T. (2019). Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8. International Biodeterioration & Biodegradation, 137, 88–94. doi:10.1016/j.ibiod.2018.11.013

Jankovska, E., Svitek, M., Holada, K. and Petrak, J. (2019) Affinity depletion versus relative protein enrichment: a side-by-side comparison of two major strategies for increasing human cerebrospinal fluid proteome coverage. Clin Proteomics, 16, 9.

Vit, O., Harant, K., Klener, P., Man, P. and Petrak, J. (2019) A three-pronged "Pitchfork" strategy enables an extensive description of the human membrane proteome and the identification of missing proteins. J Proteomics, 204, 103411.

Makukhin, N., Havelka, V., Polachova, E., Rampirova, P., Tarallo, V., Strisovsky, K. and Misek, J. (2019) Resolving oxidative damage to methionine by an unexpected membrane-associated stereoselective reductase discovered using chiral fluorescent probes. FEBS J.

Kohutova, J., Elsnicova, B., Holzerova, K., Neckar, J., Sebesta, O., Jezkova, J., Vecka, M., Vebr, P., Hornikova, D., Szeiffova Bacova, B. et al. (2018) Anti-arrhythmic Cardiac Phenotype Elicited by Chronic Intermittent Hypoxia Is Associated With Alterations in Connexin-43 Expression, Phosphorylation, and Distribution. Front Endocrinol (Lausanne), 9, 789.

Sumova, P., Sima, M., Kalouskova, B., Polanska, N., Vanek, O., Oliveira, F., … Volf, P. (2019). Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies. Insect Biochemistry and Molecular Biology, 103245. doi:10.1016/j.ibmb.2019.103245

Nosek V 1, Míšek J . Enzymatic kinetic resolution of chiral sulfoxides - an enantiocomplementary approach. Chem Commun (Camb). 2019 Aug 27;55(70):10480-10483. doi: 10.1039/c9cc05470g.

2018

Van Nguyen P., Hlavacek O., Marsikova J., Vachova L., Palkova Z. (2018) Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity. PLoS genetics, https://doi.org/10.1371/journal.pgen.1007495

.Sykora, M., Pospisek, M., Novak, J., Mrvova, S., Krasny, L. and Vopalensky, V. (2018) Transcription apparatus of the yeast virus-like elements: Architecture, function, and evolutionary origin. PLoS Pathog, 14, e1007377.

Bilkova, B., Swiderska, Z., Zita, L., Laloe, D., Charles, M., Benes, V., Stopka, P. and Vinkler, M. (2018) Domestic Fowl Breed Variation in Egg White Protein Expression: Application of Proteomics and Transcriptomics. J Agric Food Chem, 66, 11854-11863.

Melenovsky, V., Cervenka, L., Viklicky, O., Franekova, J., Havlenova, T., Behounek, M., Chmel, M. and Petrak, J. (2018) Kidney Response to Heart Failure: Proteomic Analysis of Cardiorenal Syndrome. Kidney Blood Press Res, 43, 1437-1450.

Rossmeisl, M., Pavlisova, J., Janovska, P., Kuda, O., Bardova, K., Hansikova, J., Svobodova, M., Oseeva, M., Veleba, J., Kopecky, J., Jr. et al. (2018) Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients. Biochim Biophys Acta, 1863, 712-725.

Kuda, O., Brezinova, M., Silhavy, J., Landa, V., Zidek, V., Dodia, C., Kreuchwig, F., Vrbacky, M., Balas, L., Durand, T. et al. (2018) Nrf2-Mediated Antioxidant Defense and Peroxiredoxin 6 Are Linked to Biosynthesis of Palmitic Acid Ester of 9-Hydroxystearic Acid. Diabetes, 67, 1190-1199.

Rohde, F., Schusser, B., Hron, T., Farkasova, H., Plachy, J., Hartle, S., Hejnar, J., Elleder, D. and Kaspers, B. (2018) Characterization of Chicken Tumor Necrosis Factor-alpha, a Long Missed Cytokine in Birds. Front Immunol, 9, 605.

Mach, J., Bila, J., Zeniskova, K., Arbon, D., Malych, R., Glavanakovova, M., Nyvltova, E. and Sutak, R. (2018) Iron economy in Naegleria gruberi reflects its metabolic flexibility. Int J Parasitol.

Kuntova, B., Stopkova, R. and Stopka, P. (2018) Transcriptomic and Proteomic Profiling Revealed High Proportions of Odorant Binding and Antimicrobial Defense Proteins in Olfactory Tissues of the House Mouse. Front Genet, 9, 26.

Pyrihová E, Motycková A, Voleman L, Wandyszewska N, Fišer R, Seydlová G, Roger A, Kolísko M, Doležal P. A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes. Genome Biol Evol. 2018 Oct 1;10(10):2813-2822. doi: 10.1093/gbe/evy215. PMID: 30265292; PMCID: PMC6200312.

2017

Cerna M, Kuntova B, Talacko P, Stopkova R, and Stopka P. 2017. Differential regulation of vaginal lipocalins (OBP, MUP) during the estrous cycle of the house mouse. Sci Rep 7:11674.

Erban, T., Harant, K., Chalupnikova, J., Kocourek, F. and Stara, J. (2017) Beyond the survival and death of the deltamethrin-threatened pollen beetle Meligethes aeneus: An in-depth proteomic study employing a transcriptome database. J Proteomics, 150, 281-289.

Stopkova R, Klempt P, Kuntova B, and Stopka P. 2017. On the tear proteome of the house mouse (Mus musculus musculus) in relation to chemical signalling. PeerJ 6:e3541.

Valis, K., Grobarova, V., Hernychova, L., Buganova, M., Kavan, D., Kalous, M., Cerny, J., Stodulkova, E., Kuzma, M., Flieger, M. et al. (2017) Reprogramming of leukemic cell metabolism through the naphthoquinonic compound Quambalarine B. Oncotarget, 8, 103137-103153.

Stafkova, J., Rada, P., Meloni, D., Zarsky, V., Smutna, T., Zimmann, N., Harant, K., Pompach, P., Hrdy, I. and Tachezy, J. (2017) Dynamic secretome of Trichomonas vaginalis: Case study of beta-amylases. Mol Cell Proteomics.

Vyklicka, L., Boukalova, S., Macikova, L., Chvojka, S. and Vlachova, V. (2017) The human transient receptor potential vanilloid 3 channel is sensitized via the ERK pathway. J Biol Chem.

Vosahlikova, M., Ujcikova, H., Chernyavskiy, O., Brejchova, J., Roubalova, L., Alda, M. and Svoboda, P. (2017) Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane. Biochim Biophys Acta, 1861, 1099-1112.

Laššuthová P, Gregor M, Sarnová L, Machalová E, Sedláček R, Seeman P: Clinical, In Silico, and Experimental Evidence for Pathogenicity of Two Novel Splice Site Mutations in the SH3TC2 Gene. J Neurogenet 2012 26(3-4): 413-20. [pubmed] [doi]

2016

Hartmannova, H., Piherova, L., Tauchmannova, K., Kidd, K., Acott, P.D., Crocker, J.F., Oussedik, Y., Mallet, M., Hodanova, K., Stranecky, V. et al. (2016) Acadian variant of Fanconi syndrome is caused by mitochondrial respiratory chain complex I deficiency due to a non-coding mutation in complex I assembly factor NDUFAF6. Hum Mol Genet, 25, 4062-4079.

Manakov, D., Ujcikova, H., Pravenec, M. and Novotny, J. (2016) Alterations in the cardiac proteome of the spontaneously hypertensive rat induced by transgenic expression of CD36. J Proteomics, 145, 177-186.

Stopka P, Kuntova B, Klempt P, Havrdova L, Cerna M, and Stopkova R. 2016. On the saliva proteome of the Eastern European house mouse (Mus musculus musculus) focusing on sexual signalling and immunity. Sci Rep 6:32481.

Vit, O., Man, P., Kadek, A., Hausner, J., Sklenar, J., Harant, K., Novak, P., Scigelova, M., Woffendin, G. and Petrak, J. (2016) Large-scale identification of membrane proteins based on analysis of trypsin-protected transmembrane segments. J Proteomics, 149, 15-22.

Ujcikova, H., Vosahlikova, M., Roubalova, L. and Svoboda, P. (2016) Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10days; comparison with animals exposed to morphine and subsequently nurtured for 20days in the absence of this drug. J Proteomics, 145, 11-23.

Jankovicova J, Frolikova M, Sebkova N, Simon M, Cupperova P, Lipcseyova D, Michalkova K, Horovska L, Sedlacek R, Stopka P, Antalikova J, Dvorakova-Hortova K. Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes. Reproduction. 2016 Dec;152(6):785-793. doi: 10.1530/REP-16-0304. Epub 2016 Sep 27. PMID: 27679865.

Team

Mgr. Karel Harant Mgr. Karel Harant

Mgr.
Karel Harant

Head of Proteomic Core Facilty

karel.harant@natur.cuni.cz
+420325873925
Mgr. Pavel Talacko Mgr. Pavel Talacko

Mgr.
Pavel Talacko

Non-targeted proteomics, targeted proteomics

pavel.talacko@natur.cuni.cz
+420325873925
RNDr. Petr Žáček, Ph.D. RNDr. Petr Žáček, Ph.D.

RNDr.
Petr Žáček, Ph.D.

GC / MS specialist, small molecule analysis

zacek@natur.cuni.cz
+420325873925
Mgr. Veronika Ševců Mgr. Veronika Ševců

Mgr.
Veronika Ševců

researcher, preparation of proteomic samples

veronika.sevcu@natur.cuni.cz
+420325873925