Media preparation and washing units

Media preparation and washing units

Richard Kyselý

Richard Kyselý — Laboratory head

The Institute of Biotechnology of the Czech Academy of Sciences

About us

The washing unit offers washing of laboratory glass and plastic, provides central washing of working clothing, GMO waste decontamination and elimination of hazardous waste.

The media preparation unit offers preparation of cultivation media and solutions for tissue culture, preparation of bacteriological media and plates, and preparation of “custom-made” solutions. Further, the unit offers vapour sterilization of solutions and vapour or hot-air sterilization of material, as well as dry ice supplies.

Both these units are located on the basement floor of building SO 001. The space is divided into two parts – briefly, to the “dirty” corridor, where dirty glass, clothing and waste for decontamination should be deposited, and the “clean” part, where the washed glass, sterile packed material, clean clothing, etc., is returned to the users.


The media preparation unit

We offers

  • Preparation of media for tissue culture from supplied powder media
  • Preparation of tissue culture solutions (TC H2O, PBS 1x, PBS 10x, EDTA 0.02% in PBS, trypsin 0.5% in PBS, glutamine 3%, NEA 100x, HBSS 1x with Ca, Mg, HBSS 1x without Ca, Mg, and other)
  • Preparation of bacteriological media and plates (LB, plates with ampicillin, kanamycin, without ATB or with ATB supplied according to requirements)
  • Sterile filtration of solutions
  • Sterilization of solutions and material
  • Preparation of solutions from chemicals provided by the laboratory, measurements of osmolality, pH adjustment, preparation of ATB-containing plates according to the laboratory specifications


Hot-air sterilizer VENTICELL

Washing and disinfectant automated instrument MIELE

Osmomat GONOTEC 3000 Basic

Vapour sterilizers STERIVAP HP IL 9618

Water processing - Milli Q A+