Auditory Function in Mutant Mice

Auditory Function in Mutant Mice

RNDr. Jiří Popelář, Csc.

RNDr. Jiří Popelář, Csc. — Project head

The Institute of Experimental Medicine of the Czech Academy of Sciences

About us

Hearing impairment is the most frequent inborn sensory defect. One out of every 500 children is born deaf. A large percentage of the hearing losses are monogenic. As the population is aging, the progressive hearing loss which attacks the majority of the population in old age is becoming an even more serious problem and it has its background in the pathological changes of the genome. In both cases, the mice models are of significant use when studying the genetic principles of the pathological conditions.

Recently, studies have begun on the hearing changes in some strains of laboratory rats. The defects are mostly related to the inner ear and are easily detectable by the examination of auditory stem potentials (ABR). But it is also possible to perform the screening with the help of the shock-induced reaction behavior (Startle Reflex) and its modification (PPI – Prepulse Inhibition) and to complete the examination with the observation of otoacoustic emission (DPOAE – distortion product oto-acoustic emissions) that inform about the function of receptor hair cells in the inner ear. For more detailed examination, confocal and electron microscopy, immunocytochemistry and other approaches are necessary.

In cooperation with the Transgenic and Archival Module of the Czech Centre for Phenogenomics (CCP), it will be possible to create targeted deletions of mouse mutants for certain genes suspected to play a role in inherited hearing loss and hearing impairment during the organism‘s aging. In case of suspected hearing loss in mice passing through the phenotypization in the mouse clinic, it will be possible to complete the „classic“ examinations (ABR, DPOAE, startle) even with the detailed morphological and histochemical studies.



Balogová Z, Popelář J, Chiumenti F, Chumak T, Burianová JS, Rybalko N, Syka J. Age-Related Differences in Hearing Function and Cochlear Morphology between Male and Female Fischer 344 Rats. Front Aging Neurosci. 2018 Jan 4;9:428. doi: 10.3389/fnagi.2017.00428.

Macova I, Pysanenko K, Chumak T, Dvorakova M, Bohuslavova R, Syka J, Fritzsch B, Pavlinkova G. Neurod1 is essential for the primary tonotopic organization and related auditory information processing in the midbrain. J Neurosci. 2018 Dec 12. pii: 2557-18. doi: 10.1523/JNEUROSCI.2557-18.2018.


Popelář J, Díaz Gómez M, Lindovský J, Rybalko N, Burianová J, Oohashi T, Syka
J. The absence of brain-specific link protein Bral2 in perineuronal nets hampers
auditory temporal resolution and neural adaptation in mice. Physiol Res. 2017 Nov
24;66(5):867-880. Epub 2017 Oct 11. PubMed PMID: 29020454.

Popelář J, Díaz Gómez M, Lindovský J, Rybalko N, Burianová J, Oohashi T, Syka J. Resveratrol alleviates ethanol-induced hormonal and metabolic disturbances in the rat. Physiol Res. 2017 Jul 18. PubMed PMID: 28730834.

Bureš Z, Popelář J, Syka J. The effect of noise exposure during the
developmental period on the function of the auditory system. Hear Res. 2017
Sep;352:1-11. doi: 10.1016/j.heares.2016.03.008. Epub 2016 Mar 16. Review. PubMed
PMID: 26994659.


Popelář J, Šuta D, Lindovský J, Bureš Z, Pysanenko K, Chumak T, Syka J. Cooling of the auditory cortex modifies neuronal activity in the inferior colliculus in rats. Hear Res. 2016 Feb;332:7-16. doi: 10.1016/j.heares.2015.10.021. Epub 2015 Nov 26. PubMed PMID: 26631689.

Skoloudik L, Chrobok V, Kalfert D, Koci Z, Sykova E, Chumak T, Popelar J, Syka J, Laco J, Dedková J, Dayanithi G, Filip S. Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model. Cell Transplant. 2016;25(7):1405-14. doi: 10.3727/096368915X689730. Epub 2015 Oct 22.

Chumak T, Rüttiger L, Lee SC, Campanelli D, Zuccotti A, Singer W, Popelář J, Gutsche K, Geisler HS, Schraven SP, Jaumann M, Panford-Walsh R, Hu J, Schimmang T, Zimmermann U, Syka J, Knipper M. BDNF in Lower Brain Parts Modifies Auditory Fiber Activity to Gain Fidelity but Increases the Risk for Generation of Central Noise After Injury. Mol Neurobiol. 2016 Oct;53(8):5607-27.

Šuta D, Rybalko N, Shen DW, Popelář J, Poon PW, Syka J. Frequency discrimination in rats exposed to noise as juveniles. Physiol Behav. 2015 May 15;144:60-5. doi: 10.1016


Rybalko N, Chumak T, Bureš Z, Popelář J, Šuta D, Syka J. Development of the acoustic startle response in rats and its change after early acoustic trauma. Behav Brain Res. 2015 Jun 1;286:212-21. doi: 10.1016


Bureš Z, Bartošová J, Lindovský J, Chumak T, Popelář J, Syka J. Acoustical enrichment during early postnatal development changes response properties of inferior colliculus neurons in rats. Eur J Neurosci. 2014 Dec;40(11):3674-83. doi: 10.1111


Popelář J, Rybalko N, Burianová J, Schwaller B, Syka J. The effect of parvalbumin deficiency on the acoustic startle response and prepulse inhibition in mice. Neurosci Lett. 2013 Oct 11;553:216-20. doi:10.1016

Suta D, Popelář J, Burianová J, Syka J. Cortical representation of species-specific vocalizations in Guinea pig. PLoS One. 2013 Jun 13;8(6):e65432. doi: 10.137

Groh D, Seeman P, Jilek M, Popelář J, Kabelka Z, Syka J. Hearing function in heterozygous carriers of a pathogenic GJB2 gene mutation. Physiol Res. 2013;62(3):323-30.


Rybalko N, Bureš Z, Burianová J, Popelář J, Poon PW, Syka J. Age-related changes in the acoustic startle reflex in Fischer 344 and Long Evans rats. Exp Gerontol. 2012 Dec;47(12):966-73. doi: 10.1016

Buckiová D, Ranjan S, Newman TA, Johnston AH, Sood R, Kinnunen PK, Popelář J, Chumak T, Syka J. Minimally invasive drug delivery to the cochlea through application of nanoparticles to the round window membrane. Nanomedicine (Lond). 2012 Sep;7(9):1339-54.