Synthetic Biology

Synthetic Biology

Mgr. Klára Hlouchová, Ph.D.

Mgr. Klára Hlouchová, Ph.D. — Project head

About us

The group of Synthetic Biology is affiliated with the Department of Cell Biology of the Faculty of Science, Charles University.

Synthetic biology presents tools that we use to study the vast natural and unnatural protein sequence space. Our main interests are early as well as current protein evolution. We study combinatorial peptide and protein libraries in silico, in vitro and in vivo to better understand constraints on protein structure and function evolution. We are also interested in protein engineering, novel tool development and molecular life elsewhere in the Universe.

More info www.khlab.org

Publications

2020

Tretyachenko V, Voráček V, Souček R, FujishimaK, and Hlouchová K. (2020) CoLiDe: Combinatorial Library Design tool for probing protein sequence space. Bioinformatics DOI: 10.1093/bioinformatics/btaa804

Bornberg-Bauer E, Hlouchova K, Lange A. (2020) Structure and function of naturally evolved de novo proteins. Current Opinions in Structural Biology. In Press

2019

Vymětal J, Vondrášek J, and Hlouchová, K. (2019). Sequence Versus Composition: What Prescribes IDP Biophysical Properties? Entropy, 21(7), 654. https://doi.org/10.3390/e21070654

2017

Tretyachenko V, Vymětal J, Bednárová L, Kopecký V, Hofbauerová K, Jindrová H, Hubálek M, Souček R, Konvalinka J, Vondrášek J, and Hlouchová K. (2017) Random protein sequences can form defined secondary structure and are well-tolerated in vivo. SciRep 7, 15449. https://doi.org/10.1038/s41598-017-15635-8