Laboratory of Stem Cells Proteomics

Laboratory of Stem Cells Proteomics

RNDr. Kristýna Pimková, Ph.D.

RNDr. Kristýna Pimková, Ph.D. — Project head

1st Faculty of Medicine of the Charles University

About us

Treatment of onco-hematological diseases is often complicated by the development of resistance. Cells that resist the therapy are the cause of aggressive relapse. What these cells are and how they gain the properties that allow them to overcome drug toxicity remains unclear. Elimination of these resistant leukemia cells is one of the greatest challenges in the treatment of acute myeloid leukemia.

Our laboratory is focused on basic and translational research on how redox metabolism affects the proteome dynamics of leukemia cells and their response to chemotherapy. In particular, we are interested in how changes in the redox environment affect protein function and localization. We use mass spectrometry-based proteomic approaches and combine these tools with biochemical, cell biological and genetic approaches to understand how oxidative modifications of proteins affect protein function and regulate fundamental biological processes involved in the development of resistance.




Astapenko D, Vajrychova M, Fabrik I, Kupcik R, Pimkova K, Tambor V, Radochova V, Cerny V. Impact of anesthetics on rat hippocampus and neocortex: A comprehensive proteomic study based on label-free mass spectrometry. Heliyon. 2024 Mar 5;10(6):e27638. doi: 10.1016/j.heliyon.2024.e27638. (IF 4.0)

Turkova T, Kokavec J, Zikmund T, Dibus N, Pimkova K, Nemec D, Holeckova M, Ruskova L, Sedlacek R, Cermak L, Stopka T. Differential requirements for Smarca5 expression during hematopoietic stem cell commitment. Commun Biol. 2024 Feb 29;7(1):244. doi: 10.1038/s42003-024-05917-z. (IF 5.9)


Vajrychova M, Salovska B, Pimkova K, Fabrik I, Hodny Z. SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status. Methods Mol Biol. 2023;2603:259-268. doi: 10.1007/978-1-0716-2863-8_21.


Pimkova K, Jassinskaja M, Munita R, Ciesla M, Guzzi N, Cao Thi Ngoc P, Vajrychova M, Johansson E, Bellodi C, Hansson J. Quantitative analysis of redox proteome reveals oxidation-sensitive protein thiols acting in fundamental processes of developmental hematopoiesis. Redox Biology. Volume 53, July 2022, 102343. ISSN 2213-2317. (IF 11.79)

Salovska B, Kondelova A, Pimkova K, Liblova Z, Pribyl M, Fabrik I, Bartek J, Vajrychova M, Hodny Z. Peroxiredoxin 6 protects irradiated cells from oxidative stress and shapes their senescence-associated cytokine landscape. Redox Biol. 2022 Feb;49:102212. doi: 10.1016/j.redox.2021.102212. (IF 11.79)