Group Profile (Home Institution): Department of Gene Expression (IBT ASCR)
Recent publications
Stahlberg, A., Kubista, M. Technical aspects and recommendations for single-cell qPCR. Molecular Aspects of Medicine, 59: 28-35, 2018. doi: 10.1016/j.mam.2017.07.004. ISSN: 0098-2997.
Dong LF, Kovarova J, Bajzikova M, Bezawork-Geleta A, Svec D, Endaya B, Sachaphibulkij K, Coelho AR, Sebkova N, Ruzickova A, Tan AS, Kluckova K, Judasova K, Zamecnikova K, Rychtarcikova Z, Gopalan V, Andera L, Sobol M, Yan B, Pattnaik B, Bhatraju N, Truksa J, Stopka P, Hozak P, Lam AK, Sedlacek R, Oliveira PJ, Kubista M, Agrawal A, Dvorakova-Hortova K, Rohlena J, Berridge MV, Neuzil J. Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells. Elife. 2017 Feb 15;6. pii: e22187.
Androvic, P., Valihrach, L., Elling, J., Sjoback, R., Kubista, M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Research, gkx588, 2017. doi: 10.1093/nar/gkx588. ISSN 1362-4962.
Bredemeier, M., Edimiris, P., Mach, P., Kubista, M., Sjoback, R., Rohlova, E., Kolostova, K., Hauch, S., Aktas, B., Tewes, M., Kimmig, R., Kasimir-Bauer, S. Gene Expression Signatures in Circulating Tumor Cells Correlate with Response to Therapy in Metastatic Breast Cancer. Clinical Chemistry, 63(10): 1585-1593, 2017. doi: 10.1373/clinchem.2016.269605. ISSN 0009-9147.
Forootan, A., Sjoback, R., Bjorkman, J., Sjogreen, B., Linz, L., Kubista, M. Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomolecular Detection and Quantification, [ahead of print], 2017. doi: 10.1016/j.bdq.2017.04.001. ISSN 2214-7535.
Kubista, M., Dreyer-Lamm, J., Stahlberg, A. The secrets of the cell. Molecular Aspects of Medicine, [ahead of print], 2017. doi: 10.1016/j.mam.2017.08.004. ISSN: 0098-2997.
Sindelka, R., Sidova, M., Abaffy, P., Kubista, M. Asymmetric Localization and Distribution of Factors Determining Cell Fate During Early Development of Xenopus laevis. Chapter in Asymmetric Cell Division in Development, Differentiation and Cancer, Springer International Publishing, ed. J.-P. Tassan, J. Z. Kubiak, Results and Problems in Cell Differentiation, 61: 229-241, 2017. doi: 10.1007/978-3-319-53150-2_10. ISBN 978-3-319-53149-6.
Bjorkman, J., Svec, D., Lott, E., Kubista, M., Sjoback, R. Differential amplicons (ΔAmp) - a new molecular method to assess RNA integrity. Biomolecular Detection and Quantification, Special Issue: Advanced Molecular Diagnostics for Biomaker Discovery - Part II(6): 4-12, 2016. doi: 10.1016/j.bdq.2015.09.002. ISSN 2214-7535.
Conrad, S., Azizi, H., Hatami, M., Kubista, M., Bonin, M., Hennenlotter, J., Slevert, K.-D., Skutella, T. Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells. Stem Cells International, 2016: 8582526, 2016. doi: 10.1155/2016/8582526.
Dzamba, D., Valihrach, L., Kubista, M., Anderova, M. The correlation between expression profiles measured in single cells and in traditional bulk samples. Scientific Reports, 6: 37022 , 2016. doi: 10.1038/srep37022.
Gorges, T. M., Kuske, A., Rock, K., Mauermann, O., Muller, V., Peine, S., Verpoort, K., Novosadova, V., Kubista, M., Riethdorf, S., Pantel, K. Accession of Tumor Heterogeneity by Multiplex Transcriptome Profiling of Single Circulating Tumor Cells. Clinical Chemistry, 62(11): 1504-1515, 2016. doi: 10.1373/clinchem.2016.260299. ISSN 0009-9147.
Hensler, M., Vancurova, I., Becht, E., Palata, O., Strnad, P., Tesarova, P., Cabinakova, M., Svec, D., Kubista, M., Bartunkova, J., Spisek, R., Sojka, L. Gene expression profiling of circulating tumor cells and peripheral blood mononuclear cells from breast cancer patients. OncoImmunology, 5(4): e1102827, 2016. doi: 10.1080/2162402X.2015.1102827. ISSN 2162-402X.
Honsa, P., Valny, M., Kriska, J., Matuskova, H., Harantova, L., Kirdajova, D., Valihrach, L., Androvic, P., Kubista, M., Anderova, M. Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog. GLIA, 64(9): 1518-1531, 2016. doi: 10.1002/glia.23019. ISSN 1098-1136.
Korenkova, V., Slyskova, J., Novosadova, V., Pizzamiglio, S., Langerova, L., Bjorkman, J., Vycital, O., Liska, V., Levy, M., Veskrna, K., Vodicka, P., Vodickova, L., Kubista, M., Verderio, P. The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data. Scientific Reports, 6: 29023, 2016. doi: 10.1038/srep29023.
Virant-Klun, I., Stahlberg, A., Kubista, M., Skutella, T. MicroRNAs: From Female Fertility, Germ Cells, and Stem Cells to Cancer in Humans. Stem Cells International, 2016: 3984937, 2016. doi: 10.1155/2016/3984937.
Andersson, D., Akrap, N., Svec, D., Godfrey, T. E., Kubista, M., Landberg, G., Stahlberg, A. Properties of targeted preamplification in DNA and cDNA quantification. Expert Review of Molecular Diagnostics, 15(8): 1085-1100, 2015. doi: 10.1586/14737159.2015.1057124. ISSN 1473-7159.
Kubista, M., Dreyer-Lamm, J., Stahlberg, A. The secrets of the cell. Molecular Aspects of Medicine, 59: 1-4, 2018. doi: 10.1016/j.mam.2017.08.004. ISSN: 0098-2997.