Mouse models for studying of physiology and pathophysiology of digestive epithelia

RNDr. Martin Gregor, Ph.D. — Project head

About us

Mechanosensing, intermediate filaments, cell signaling, simple epithelia, cancer

The main research focus of our group has been centered around understanding cytoskeletal organization and dynamics, cell adhesion and associated cell signaling. In particular, we are interested in plectin-dependent regulation of the actin and intermediate filament cytoskeleton, adhesion mediated signalling and cell motility.

Plectin, a ubiquitously expressed cytolinker protein, interlinks the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. In addition, plectin recruits the cytoskeleton to junctional complexes in the plasma membrane. In epithelia, plectin controls keratin cytoarchitecture and is essential for the structural integrity, mechanical strength and maintenance of paracellular barrier function.

More info HERE

Publications

2017

Jirouskova M, Nepomucka K, Oyman-Eyrilmez G, Kalendova A, Havelkova H, Sarnova L, Chalupsky K, Schuster B, Benada O, Miksatkova P, Kuchar M, Fabian O, Sedlacek R, Wiche G, Gregor M. Plectin Controls Biliary Tree Architecture and Stability in Cholestasis. Hepatol. J Hepatol. 2017 Dec 19. pii: S0168-8278(17)32495-9.

Sarnova L, Gregor M: Biliary system architecture: experimental models and visualization techniques. Physiol Res 2017 66(3): 383-390.

2016

Brauer R, Turečková J, Kanchev I, Khoylou M, Škarda J, Procházka J, Spoutil F, Beck IM, Žbodáková O, Kašpárek P, Kořínek V, Chalupský K, Karhu T, Herzig KH, Hajduch M, Gregor M, Sedláček R: MMP-19 deficiency causes aggravation of colitis due to defects in innate immune cell function. Mucosal Immunol 2016 9(4): 974-85.

Müller M, Wetzel S, Köhn-Gaone J, Chalupsky K, Lüllmann-Rauch R, Barikbin R, Bergmann J, Wöhner B, Zbodakova O, Leuschner I, Martin G, Tiegs G, Rose-John S, Sedlacek R, Tirnitz-Parker JE, Saftig P, Schmidt-Arras D: A disintegrin and metalloprotease 10 (ADAM10) is a central regulator of murine liver tissue homeostasis. Oncotarget 2016 7(14): 17431-41.

2015

Song JG, Kostan J, Drepper F, Knapp B, de Almeida Ribeiro E, Konarev PV, Grishkovskaya I, Wiche G, Gregor M, Svergun DI, Warscheid B, Djinović-Carugo K: Structural insights into Ca2+-calmodulin regulation of Plectin 1a-integrin β4 interaction in hemidesmosomes. Structure 2015 23(3): 558-70.

2014

Gregor M, Osmanagic-Myers S, Burgstaller G, Wolfram M, Fischer I, Walko G, Resch GP, Jörgl A, Herrmann H, Wiche G: Mechanosensing through focal adhesion-anchored intermediate filaments. FASEB J 2014 28(2): 715-29.

Prukova D, Ileninova Z, Antosova B, Kasparek P, Gregor M, Sedlacek R: Transgenic reporter mice with promoter region of murine LRAT specifically marks lens and meiosis spermatocytes. Physiol Res 2014.

2013

Buryova H, Chalupský K, Žbodáková O, Kanchev I, Jiroušková M, Gregor M, Sedláček R: Liver protective effect of ursodeoxycholic acid includes regulation of ADAM17 activity. BMC Gastroenterol 2013 13(1): 155.

Chalupský K, Kanchev I, Žbodáková O, Buryová H, Jiroušková M, Kořínek V, Gregor M, Sedláček R: ADAM10/17-dependent release of soluble c-Met correlates with hepatocellular damage. Folia Biol (Praha) 2013 59(2): 76-86.

2012

Jirouskova M, Zbodakova O, Gregor M, Chalupsky K, Sarnova L, Hajduch M, Ehrmann J, Jirkovska M, Sedlacek R: Hepatoprotective Effect of MMP-19 Deficiency in a Mouse Model of Chronic Liver Fibrosis. PLoS One 2012 7(10): e46271.

Laššuthová P, Gregor M, Sarnová L, Machalová E, Sedláček R, Seeman P: Clinical, In Silico, and Experimental Evidence for Pathogenicity of Two Novel Splice Site Mutations in the SH3TC2 Gene. J Neurogenet 2012 26(3-4): 413-20.