Structure of Medically and Biotechnologically Important Enzymes

Structure of Medically and Biotechnologically Important Enzymes

Ing. Jan Dohnálek, Ph.D.

Ing. Jan Dohnálek, Ph.D. — Project head

About us

Our research is focused on application of single crystal X-ray diffraction analysis on the questions of the structure-function relationship in biological macromolecules. In the recent twenty years our improved understaning of the key structural properties of proteins had enormous impact in the fields of cell and general biology, drug design or biotechnologies.

We are mainly interested in structure elucidation of new and novel enzymes, receptors, or complexes. Based on the atomic details of the structures we interpret the relationship between the structural details and function or stability of an enzyme, activity of protein complexes or interaction properties of receptor-ligand pairs. Our results include enzyme modifications (mutations) leading to their altered properties, suggestions for new ligands, potentially leading to new drugs, or entirely new knowledge on the level of structural molecular biology.

For more information please visit our home institution website.

News

Events

Publications

2015

Skalova, T., Blaha, J., Harlos, K., Duskova, J., Koval, T., Stransky, J., Hasek, J., Vanek, O., Dohnalek, J. Four crystal structures of human LLT1, a ligand for human NKR-P1, in varied glycosylation and oligomerization state. Acta Crystallographica D: Biological Crystallography, 71(Pt 3): 578-591, 2015. doi: 10.1107/S1399004714027928. eISSN 1399-0047.

2013

Koval, T., Lipovova, P., Podzimek, T., Matousek, J., Duskova, J., Skalova, T., Stepankova, A., Hasek, J., Dohnalek, J. Plant multifunctional nuclease TBN1 with unexpected phospholipase activity: structural study and reaction-mechanism analysis. Acta Crystallogr. D69: 213–226, 2013. PDB: wild type, 3sng; N211D mutant, 4dj4

2011

Koval, T., Lipovova, P., Podzimek, T., Matousek, J., Duskova, J., Skalova, T., Stepankova, A., Hasek, J., Dohnalek, J. Crystallization of recombinant bifunctional nuclease TBN1 from tomato. Acta Crystallogr. F67: 124-128, 2011.

Kolenko, P., Rozbesky, D., Vanek, O., Kopecky, V. Jr., Hofbauerova, K., Novak, P., Pompach, P., Hasek, J., Skalova, T., Bezouska, K., Dohnalek, J. Molecular architecture of mouse activating NKR-P1 receptors. J. Struct. Biol., 175(3): 434-441, 2011.

Team

Ing. Jan Dohnálek, Ph.D.

Ing.
Jan Dohnálek, Ph.D.

Head of the CMS Core Facility, Biocev representative of Czech infrastructure for integrative structural biology - CIISB, Biocev representative of European infrastructure Instruct

jan.dohnalek@ibt.cas.cz
+420325873758

Mgr.
Jarmila Dušková, Dr.

jarmila.duskova@ibt.cas.cz
+420325873759

RNDr.
Jindřich Hašek, DrSc.

jindrich.hasek@ibt.cas.cz
+420325873787

RNDr.
Tereza Skálová, Ph.D.

tereza.skalova@ibt.cas.cz
+420325873759

Mgr.
Mária Trundová, Ph.D.

maria.trundova@ibt.cas.cz
+420325873759

Ing.
Petr Kolenko, Ph.D.

petr.kolenko@ibt.cas.cz
+420325873759
Bc. Michal Strnad

Bc.
Michal Strnad

computer maintenance and IT

michal.strnad@ibt.cas.cz
+420325873759