Structural biology of signaling proteins

Structural biology of signaling proteins

RNDr. Veronika Obšilová, Ph.D.

RNDr. Veronika Obšilová, Ph.D. — Project head

About us

Our group focuses on studying protein-protein interactions with the aim to elucidate the molecular basis of regulatory mechanisms of signaling proteins and their complexes from the biologically important signaling pathways. The main focus is on 14-3-3 protein complexes with proteins involved in the regulation of apoptosis, cancer, G-protein and calcium signaling pathways.

We employ various biophysical (fluorescence spectroscopy, analytical ultracentrifugation, ITC, MST, DSF), structural (X-ray crystallography, NMR, SAXS, HDX-MS) and biochemical (site-directed mutagenesis, enzyme kinetics) approaches to understand the structure-function relationships of studied proteins and protein-protein complexes.

 

Objectives

  • Preparation, biochemical and biophysical characterization of selected signaling proteins.
  • Determination of binding affinities and stoichiometries of studied signaling complexes.
  • Structural studies of selected signaling protein complexes.

 

Group Profile (Home Institution): Fyziologický ústav AV ČR, v.v.i.

More info

Publications

2017

Alblova M, Smidova A, Docekal V, Vesely J, Herman P, Obsilova V, Obsil T. Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1. Proc Natl Acad Sci U S A. 2017, 114:E9811-E9820.

Kalabova D, Smidova A, Petrvalska O, Alblova M, Kosek D, Man P, Obsil T, Obsilova V. Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding. Biochem Biophys Res Commun. 2017, 493:940-945.

Kacirova M, Novacek J, Man P, Obsilova V, Obsil T. Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function. Biophys J. 2017, 112:1339-1349.

2016

Kylarova S, Kosek D, Petrvalska O, Psenakova K, Man P, Vecer J, Herman P, Obsilova V, Obsil T. Cysteine residues mediate high-affinity binding of thioredoxin to ASK1. FEBS J. 2016, 283:3821-3838.

Petrvalska O, Kosek D, Kukacka Z, Tosner Z, Man P, Vecer J, Herman P, Obsilova V, Obsil T. Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1). J Biol Chem. 2016, 291:20753-65.

2015

Kacirova M, Kosek D, Kadek A, Man P, Vecer J, Herman P, Obsilova V, Obsil T. Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein. J Biol Chem. 2015, 290:16246-60.

2014

Kosek D, Kylarova S, Psenakova K, Rezabkova L, Herman P, Vecer J, Obsilova V, Obsil T. Biophysical and structural characterization of the thioredoxin-binding domain of protein kinase ASK1 and its interaction with reduced thioredoxin. J Biol Chem. 2014, 289:24463-74.

Kopecka M, Kosek D, Kukacka Z, Rezabkova L, Man P, Novak P, Obsil T, Obsilova V. Role of the EF-hand-like motif in the 14-3-3 protein-mediated activation of yeast neutral trehalase Nth1. J Biol Chem. 2014, 289:13948-61.

2013

Macakova E, Kopecka M, Kukacka Z, Veisova D, Novak P, Man P, Obsil T, Obsilova V. Structural basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1. Biochim Biophys Acta. 2013, 1830:4491-9.

2012

Veisova D, Macakova E, Rezabkova L, Sulc M, Vacha P, Sychrova H, Obsil T, Obsilova V. Role of individual phosphorylation sites for the 14-3-3-protein-dependent activation of yeast neutral trehalase Nth1. Biochem J. 2012,443:663-670.

Team

RNDr. Veronika Obšilová, Ph.D.

RNDr.
Veronika Obšilová, Ph.D.

Head of Group Structural biology of signaling proteins

veronika.obsilova@fgu.cas.cz
+420325873513, +420325873507
Prof. RNDR. Tomáš Obšil, Ph.D.

Prof. RNDR.
Tomáš Obšil, Ph.D.

obsil@natur.cuni.cz
+420325873513, +420325873507
Mgr. Matěj Horváth, Ph.D.

Mgr.
Matěj Horváth, Ph.D.

matej.horvath@fgu.cas.cz
+420325873506
Mgr. Dana Kalábová

Mgr.
Dana Kalábová

PhD Student

dana.kalabova@fgu.cas.cz
+420325873506
Mgr. Olívia Petrvalská

Mgr.
Olívia Petrvalská

Postdok

olivia.petrvalska@fgu.cas.cz
+420325873506
Bc. Karolína Honzejková

Bc.
Karolína Honzejková

Student

karolina.honzejkova@seznam.cz
+420325873506
Klára Kohoutová

Klára Kohoutová

Student

klara19952018@gmail.com
+420325873506
Mgr. Pavel Pohl

Mgr.
Pavel Pohl

PhD Student

pavel.pohl@fgu.cas.cz