The aim of this Project is to understand how light controls the structure, dynamics and activity of photosensitive proteins.
We are particularly interested in transcription factors that interact with specific sequences of DNA in a light-dependent manner. We employ a combination of computational (density functional theory, quantum mechanics/molecular mechanics) and time-resolved structural biology techniques, including spectroscopy (infrared, Raman and fluorescence), diffraction (X-ray crystallography) and scattering (small-angle X-ray scattering). This Project has a close collaboration with the “Intermolecular Recognition of Proteins and Nucleic Acids” Project and the “Structure of Medically and Biotechnologically Important Enzymes” Project.
- Elucidation of the sequence of molecular events leading to the formation of protein/DNA complexes upon photoexcitation.
- Development of molecular engineering tools for the site-specific labeling of proteins.
- Development of computational tools for the analysis of photoinduced processes in proteins and nucleic acids.