Stem cells in epidermis and their use in tissue engineering

Stem cells in epidermis and their use in tissue engineering

Prof. MUDr. Karel Smetana, DrSc.

Prof. MUDr. Karel Smetana, DrSc. — Project head

About us

he project is aimed at studying possibilities of epidermal stem cell isolation. The epidermal stem cells and the multipotent neural crest originated stem cells represent the most easily accessible stem cells in human body. The in vitro propagation and controled differentiation will be investigated too.

Our interest is focussed predominantly on study of the microenvironment of these cells under physiological and pathological conditions (cancer) that is necesery for their expansion in vitro.

 

Research Objectives:

  • Development of separation and cultivation methods for the preparation of stem cells from the human epidermis
  • Methods of the characterization and differentiation of the obtained cell types
  • Study of the influence of the microenvironment on the stem cells function and their differentiation
  • Development of the new clinical applications

More info at: Skin stem cell lab

Further information can be found at www.phenogenomics.cz or at the link https://biocev.lf1.cuni.cz/

News

Publications

2018

Solís D, Bovin NV, Davis AP, Jiménez-Barbero J, Romero A, Roy R, Smetana K Jr, Gabius HJ. A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code. Biochim Biophys Acta. 2015 Jan;1850(1):186-235.

Lacina L, Kodet O, Dvořánková B, Szabo P, Smetana K Jr.: Ecology of melanoma cell. Histol Histopathol 33: 247-254 (2018) (IF 2.025)

Kodet O., Dvořánková B., Bendlová B., Sýkorová V., Krajsová I., Štork J, Kučera J., Szabo P., Strnad H., Kolář M., Vlček Č., Smetana K. Jr., Lacina L.: Microenvironment‑driven resistance to B ‑Raf inhibition in a melanoma patient is accompanied by broad changes of gene methylation and expression in distal fibroblasts. Int J Mol Med 41: 3687-2703 (2018). (IF 2.341)

2017

Gál P, Varinská L, Fáber L, Novák Š, Szabo P, Mitrengová P, Mirossay A, Mučaji P, Smetana K. Jr.: How signaling molecules regulate tumor microenvironment: Parallels to wound Repair. Molecules. 22: pii: E1818 (2017) (IF 2.861)

2016

Smetana K., jr. Lacina L., Szabo, P., Dvořánková, B., Brož, P., Šedo, A.: Ageing as an important risk factor for cancer. Anticancer Res 36: 5009-5018 (2016) (IF 1.895)

2015

Kodet, O., Lacina, L., Krejčí, E., Dvořánková, B., Grim, M., Štork, J., Kodetová, D., Vlček, Č., Šáchová, J., Kolář, M., Strnad, H., Smetana, K.: Melanoma cells influence the differentiation pattern of human epidermal keratinocytes. Mol Cancer. doi: 10.1186/1476-4598-14-1 (2015)

Smetana K Jr, Szabo P, Gal P, André S, Gabius HJ, Kodet O, Dvořánková B. Emerging role of tissue lectins as microenvironmental effectors in tumors and wounds. Histol Histopathol. 2015 Mar;30(3):293-309.

Lacina, L. Plzak J., Kodet O., Szabo P., Chovanec, M., Dvorankova B., Smetana, K. Jr.: Cancer microenvironment: What can we learn from the stem cell niche. Int J Mol Sci 16: 24094-24110 (2015) (IF 2. 862)

2013

Smetana, K, Jr et al: Role of stromal fibroblasts in cancer originated from squamous epithelia. In Bai, X. (ed) Dermal Fibroblasts: Histological Perspectives, Characterization and Role in Disease. Nova Sciences Publishers, New York (2013) pp. 83-94

Team

Prof. MUDr. Karel Smetana, DrSc.

Prof. MUDr.
Karel Smetana, DrSc.

Head of Group Stem Cells in Epidermis and Their Application in Cell Therapy

karel.smetana@lf1.cuni.cz
+420325873021, +420325873009

RNDr.
Barbora Dvořánková, Ph.D.

barbora.dvorankova@lf1.cuni.cz
+420325873021, +420325873009