Polymeric Biomaterials for Regenerative Medicine

Polymeric Biomaterials for Regenerative Medicine

Mgr. Dana Kubies, CSc.

Mgr. Dana Kubies, CSc. — Project head

About us

The important factor supporting integration of biomaterials with the tissue of recipient in biomedical applications is immobilization of biologically active substances on the biomaterial surface. Our research activities are focused on development of technologies for surface modifications. The studied coating technologies are tools for controlled introduction of biological active substances (such as specific peptides or growth factors) on the surface of porous as well as nonporous implants.

In this way we aim to modulate the interaction of the implant surface with the host tissue in the place of implantation (e.g. adsorption of proteins, adhesion, proliferation or differentiation of cells or surface endothelization). We also focus on preparation of 3D macroporous polymer scaffolds for cell transplantation and for tissue regeneration and replacement applications.

Research areas

  • Surface modification of biomaterials for increase of their bioactivity
  • Preparation of 3D polymer scaffolds for tissue engineering applications
     

Research projects

  • Coatings deposited on the surface of biomaterials via physical interactions
    • Coatings based on self-associated complexes of polyelectrolytes enabling delivery of growth factors and cytokines into the place of implantation. (layer-by-layer approach)
    • Coatings based on deposition of colloidal solutions of functionalized block copolymers containing specific peptide sequences and growth factors
  • Coatings deposited on the surface of biomaterials via polymerization techniques
    • Multifunctional combined coatings prepared by controlled polymerization of fibrinogen containing growth factors and antithrombogenic agens. Deposition of organized fibrin nanostructures growing from the surface by a catalytic action of the surface-bound thrombin on the solution containing fibrinogen and subsequent covalent and non-covalent immobilization of growth factors
    • Multifunctional combined coatings prepared by advanced polymerization techniques  (surface initiated controlled polymerizations) yielding grafted polymer brushes with bioactive species (antitrombogenic agens, specific peptide sequences, aptamers)
  •  Polyelectrolyte particles based on natural polymers as carriers for delivery of growth factors and cytokines

Publications

2017

Mázl Chánová et al. Polymer brushes based on PLLA-b-PEO colloids for the preparation of protein resistant PLA surfaces. Biomater. Sci. 2017, 5, 1130

Riedel T, Hageneder S, Surman F, Pop-Georgievski O, Noehammer C, Hofner M, Brynda E, Rodriguez-Emmenegger C, Dostálek J. Plasmonic Hepatitis B Biosensor for the Analysis of Clinical Saliva. Anal Chem. 2017 Mar 7;89(5):2972-2977.

Bacakova M, Pajorova J, Stranska D, Hadraba D, Lopot F, Riedel T, Brynda E, Zaloudkova M, Bacakova L. Protein nanocoatings on synthetic polymeric nanofibrous membranes designed as carriers for skin cells. Int J Nanomedicine. 2017 Feb 9;12:1143-1160.

Kaplan, Ondrej; Zarubova, Jana; Mikulova, Barbora, Brynda; et al.Enhanced Mitogenic Activity of Recombinant Human Vascular Endothelial Growth Factor VEGF(121) Expressed in E. coli Origami B (DE3) with Molecular Chaperones, PLOS ONE ,12(1), Article Number: e0169590 , 2017

Kaplan O, Hierlemann T, Krajewski S, Kurz J, Nevoralová M, Houska M, Riedel T, Riedelová Z, Zárubová J, Wendel HP, Brynda E. Low-thrombogenic fibrin-heparin coating promotes in vitro endothelialization. J Biomed Mater Res A. 2017 Nov;105(11):2995-3005.

Kumorek et al. Effect of crosslinking chemistry of albumin/heparin multilayers on FGF-2 adsorption and endothelial cell behavior. Applied Surface Science 2017, 411, 240.

2016

Kasoju, N. Kubies, D.Sedlačík, T, Janoušková, O, Koubková, J., Kumorek, M.M., Rypáček, F. Polymer scaffolds with no skin-effect for tissue engineering applications fabricated by thermally induced phase separation, Biomedical Materials, 11 (1), Article number 015002, 2016

Z. Riedelová-Reicheltová, Brynda E., Riedel T. Fibrin nanostructures for biomedical applications. Physiological Research 2016; 65: S263-S272.

Himmlova, Lucie; Kubies, Dana; Hulejova, Hana; et al, Effect of Blood Component Coatings of Enosseal Implants on Proliferation and Synthetic Activity of Human Osteoblasts and Cytokine Production of Peripheral Blood Mononuclear Cell, MEDIATORS OF INFLAMMATION , Article Number: 8769347, 2016

2015

Mázl Chánová et al. , Nano-Colloid Printing of Functionalized PLA-b-PEO Copolymers: Tailoring the Surface Pattern of Adhesive Motif and its Effect on Cell Attachment, Physiological research 64, S61-S73, 2015

Kumorek et al. Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies, PLOS ONE 10(5), Article Number: e0125484, 2015

Team

Mgr. Dana Kubies, CSc.

Mgr.
Dana Kubies, CSc.

Head of Group Polymeric Biomaterials for Regenerative Medicine

kubies@imc.cas.cz
+420325873800, +420325873809

Ing.
Jiří Michálek, CSc.

jiri@imc.cas.cz
RNDr. Eduard Brynda, CSc.

RNDr.
Eduard Brynda, CSc.

Member of BIOCEV Internal Scientific Board, Co-ordinator of research programme Biomaterials and Tissue Engineering

brynda@imc.cas.cz
+420325873809, +420325873810
Ing. Edyta Paula Wawrzyńska, Ph.D.

Ing.
Edyta Paula Wawrzyńska, Ph.D.

wawrzynska@imc.cas.cz
+420325873800
Ing. Eliška Mázl-Chánová, Ph.D.

Ing.
Eliška Mázl-Chánová, Ph.D.

chanova@imc.cas.cz
+420325873800
Ing. Zuzana Riedelová, Ph.D.

Ing.
Zuzana Riedelová, Ph.D.

riedelova@imc.cas.cz
+420325873800
Ing. Tomáš  Riedel, Ph.D.

Ing.
Tomáš Riedel, Ph.D.

riedel@imc.cas.c
Mgr. Johanka Kučerová, Ph.D.

Mgr.
Johanka Kučerová, Ph.D.

kucerova@imc.cas.cz
+420325873801, +420325873802
Jakub Doležal

Jakub Doležal

Technical Expert

dolezal@imc.cas.cz
+420325873801