Application of Stem Cells and Biomaterials in Cell Therapy

Application of Stem Cells and Biomaterials in Cell Therapy

Doc. RNDr. Pavla Jendelová, Ph.D.

Doc. RNDr. Pavla Jendelová, Ph.D. — Project head

About us

The group focuses on the research and development of new treatments for central nervous system (CNS) injury with potential use in human medicine. We deal with the use of stem cells (neural precursors, multipotent mesenchymal stromal cells) and polymeric hydrogels to treat brain and spinal cord injuries.

The development and application of 3D macroporous hydrogel scaffolds (based on different natural or synthetic biomaterials) provide the necessary architecture for the cell growth in vitro and targeted cell support that promote regeneration of the injured tissues after implantation. The 3D organization of the cell- scaffolds constructs more closely mimics the microenvironment occurred in vivo that opens additional opportunities for the development of 3D in vitro modelling systems.

We are working on the improvement of in vitro cell culture parameters and optimization of hypothermic/cryogenic storage conditions to ensure the maintenance of therapeutic potential of stem cells for clinical applications.

Another potential therapeutic target in the treatment of nervous system injury is microRNA. The main objectives of the project include the detection of the role of microRNA in the pathophysiology of spinal cord injury and stroke and the study of their possible therapeutic use.

Research objectives:

  • analysis of the temporal expression of miRNA in the nervous tissue following CNS injuries
  • validation of individual miRNAs in vitro in a relevant cell context
  • proving the therapeutic implications of specific microRNAs in vivo
  • studying the role of signal pathways (NF-κB, mTOR) in stem cell differentiation and spinal cord injury

Official website

Publications

2017

Machová-Urdzíková, L., Růžička, J., Kárová, K., Kloudová, A., Svobodová, B., Anubhav, A., Dubišová, J., Schmidt, M., Kubinová, Š., Jhanwar Uniyal, M., Jendelová, P.: (2017) A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines. Neuropharmacology. 126: 213-223.

Růžička J., Machová Urdziková L, Gillick J, Amemori T, Romayuk N, Kárová K, et al.: (2017) A Comparative Study of Three Different Types of Stem Cells for Treatment of Rat Spinal Cord Injury. Cell Transplant. 2017;26(4): 585-603.

2015

Romanyuk N, Amemori T, Turnovcova K, Prochazka P, Onteniente B, Sykova E, Jendelova P. Beneficial Effect of Human Induced Pluripotent Stem Cell-Derived Neural Precursors in Spinal Cord Injury Repair. Cell Transplant. 2015;24(9):1781-97.

Team

Doc. RNDr. Pavla Jendelová, Ph.D.

Doc. RNDr.
Pavla Jendelová, Ph.D.

Head of Group

pavla.jendelova@iem.cas.cz
+420325873407
Mgr. Nataliya Romanyuk, Ph.D.

Mgr.
Nataliya Romanyuk, Ph.D.

Research assistant

nataliya.romanyuk@iem.cas.cz
+420325873407
Mgr. Kristýna Kárová, Ph.D.

Mgr.
Kristýna Kárová, Ph.D.

Postdoc

karova@biomed.cas.cz
Mgr. Barbora Svobodová

Mgr.
Barbora Svobodová

PhD Student

barbora.svobodova@biomed.cas.cz
Mgr. Yuriy Petrenko, Ph.D.

Mgr.
Yuriy Petrenko, Ph.D.

Research assistant

yuri.petrenko@iem.cas.cz
+420325873407
Mgr. Ingrid Vargová

Mgr.
Ingrid Vargová

PhD. student

ingrid.vargova@biomed.cas.cz
+420325873407
Mgr. Ivan Arzhanov

Mgr.
Ivan Arzhanov

PhD. Student

ivan.arzhanov@iem.cas.cz

Ing.
Irena Vacková, Csc.

Research Assistant

irena.vackova@iem.cas.cz

Zuzana Čermáková

Technician

zuzana.cermakova@iem.cas.cz
+420325873406

Teaching

Offered topics

thesis author thesis name thesis type
The role of microRNA in the regulation of gene expression after spinal cord injury and its validation at protein level Diploma Thesis
The role of microRNA in injury and regeneration of nerve tissue Bachelor Thesis