Recent publications


Koval, T., Dohnalek, J. Characteristics and application of S1-P1 nucleases in biotechnology and medicine. Biotechnology Advances, [ahead of print], 2017. doi: 10.1016/j.biotechadv.2017.12.007. ISSN 0734-9750.

Stranava, M., Man, P., Skalova, T., Kolenko, P., Blaha, J., Fojtikova, V., Martinek, V., Dohnalek, J., Lengalova, A., Rosulek, M., Shimizu, T., Martinkova, M. Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction. Journal of Biological Chemistry, 20921-20935, 2017. doi: 10.1074/jbc.M117.817023. eISSN 1083-351X.


Agirre, J., Ariza, A., Offen, W. A., Turkenburg, J. P., Roberts, S. M., McNicholas, S., Harris, P. V., McBrayer, B., Dohnalek, J., Cowtan, K. D., Davies, G. J., Wilson, K. S. Three-dimensional structures of two heavily N-glycosylated Aspergillus sp. family GH3 β-D-glucosidases. Acta Crystallographica Section D, 72: 254-265, 2016. doi: 10.1107/S2059798315024237. ISSN 2059-7983.

Dohnalek, J., McAuley, K. E., Brzozowski, A. M., Ostergaard, P. R., Svendsen, A., Wilson, K. S. Stabilization of Enzymes by Metal Binding: Structures of Two Alkalophilic Bacillus Subtilases and Analysis of the Second Metal-Binding Site of the Subtilase Family. Chapter in Understanding enzymes: Function, Design, Engineering and Analysis, Pan Stanford Publishing, ed. A. Svendsen, 203-266, 2016. doi: 10.4032/9789814669337. ISBN 789814669320.

Fejfarova, K., Kadek, A., Mrazek, H., Hausner, J., Tretyachenko, V., Koval, T., Man, P., Hasek, J., Dohnalek, J. Crystallization of nepenthesin I using a low-pH crystallization screen. Acta Crystallographica Section F, 72: 24-28, 2016. doi: 10.1107/S2053230X15022323. ISSN 2053-230X.

Koval, T., Ostergaard, L. H., Lehmbeck, J., Norgaard, A., Lipovova, P., Duskova, J., Skalova, T., Trundova, M., Kolenko, P., Fejfarova, K., Stransky, J., Svecova, L., Hasek, J., Dohnalek, J. Structural and Catalytic Properties of S1 Nuclease from Aspergillus oryzae Responsible for Substrate Recognition, Cleavage, Non-Specificity, and Inhibition. PLoS ONE 11(12): e0168832, 2016. doi: 10.1371/journal.pone.0168832. ISSN 1932-6203.


Skalova, T., Blaha, J., Harlos, K., Duskova, J., Koval, T., Stransky, J., Hasek, J., Vanek, O., Dohnalek, J. Four crystal structures of human LLT1, a ligand for human NKR-P1, in varied glycosylation and oligomerization state. Acta Crystallographica D: Biological Crystallography, 71(Pt 3): 578-591, 2015. doi: 10.1107/S1399004714027928. eISSN 1399-0047.

Stransky, J., Koval, T., Podzimek, T., Tycova, A., Lipovova, P., Matousek, J., Kolenko, P., Fejfarova, K., Duskova, J., Skalova, T., Hasek, J., Dohnalek, J. Phosphate binding in the active centre of tomato multifunctional nuclease TBN1 and analysis of superhelix formation by the enzyme. Acta Crystallographica Section F, 71: 1408-1415, 2015. doi: 10.1107/S2053230X15018324. ISSN 2053-230X.


Wiedermannova, J., Sudzinova, P., Koval, T., Rabatinova, A., Sanderova, H., Ramaniuk, O., Rittich, S., Dohnalek, J., Fu, Z., Halada, P., Lewis, P., Krasny, L. Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis. Nucleic Acids Research, 42(8): 5151-5163, 2014. doi: 10.1093/nar/gku113. ISSN 0305-1048.


Stepankova, A., Duskova, J., Skalova, T., Hasek, J., Koval, T., Ostergaard L. H., Dohnalek, J. Organophosphorous acid anhydrolase from Alteromonas macleodii - a structural study and functional relationship to prolidases. Acta Crystallogr. F69: 346-354, 2013. PDB: 3RVA

Koval, T., Lipovova, P., Podzimek, T., Matousek, J., Duskova, J., Skalova, T., Stepankova, A., Hasek, J., Dohnalek, J. Plant multifunctional nuclease TBN1 with unexpected phospholipase activity: structural study and reaction-mechanism analysis. Acta Crystallogr. D69: 213–226, 2013. PDB: wild type, 3sng; N211D mutant, 4dj4


Skalova, T., Kotynkova, K., Duskova, J., Hasek, J., Koval, T., Kolenko, P., Novak, P., Man, P., Hanc, P., Vanek, O., Bezouska, P., Dohnalek., J. Mouse Clr-g, a Ligand for NK Cell Activation Receptor NKR-P1F: Crystal Structure and Biophysical Properties. J. of Immunology, 189 (10): 4881-4889, 2012. PDB: 3RS1


Hasek, J., Labsky, J., Skalova, T., Kolenko, P., Dohnalek, J., Duskova, J., Stepankova, A., Koval, T. Polymer structure database and protein-polymer interactions. Z. Kristallogr. Proc., 1: 475-480, 2011.

Tishchenko, G., Simunek, J., Bartonová, H., Duskova, J., Dohnalek, J., Ponomareva, E., Tennikova, T. Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids. J. Chromatogr. B Analyt. Technol. Biomed Life Sci, 879(22): 2175-2178, 2011.

Kolenko, P., Rozbesky, D., Vanek, O., Bezouska, K., Hasek, J., Dohnalek, J. Structure of the H107R variant of the extracellular domain of mouse NKR-P1A at 2.3 Å resolution. Acta Crystallogr. F67: 1519-1523, 2011. PDB: 3t3a

Kolenko, P., Rozbesky, D., Vanek, O., Kopecky, V. Jr., Hofbauerova, K., Novak, P., Pompach, P., Hasek, J., Skalova, T., Bezouska, K., Dohnalek, J. Molecular architecture of mouse activating NKR-P1 receptors. J. Struct. Biol., 175(3): 434-441, 2011.

Duskova, J., Tishchenko, G., Ponomareva, E., Simunek, J., Koppova, I., Skalova, T., Stepankova, A., Hasek, J., Dohnalek, J. Chitinolytic enzymes from bacterium inhabiting human gastrointestinal tract - critical parameters of protein isolation from anaerobic culture. Acta Biochimica Polonica 58(2): 261–263, 2011.

Koval, T., Lipovova, P., Podzimek, T., Matousek, J., Duskova, J., Skalova, T., Stepankova, A., Hasek, J., Dohnalek, J. Crystallization of recombinant bifunctional nuclease TBN1 from tomato. Acta Crystallogr. F67: 124-128, 2011.

Skalova, T., Duskova, J., Hasek, J., Stepankova, A., Koval, T., Ostergaard, L. H., Dohnalek, J. Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 A. Acta Crystallogr. F67: 27-32, 2011. PDB: 3KW8


Tishchenko, G., Koppova, I., Simunek, J., Dohnalek, J. Extracellular Complex of Chitinolytic Enzymes of Clostridium paraputrificum Strain J4 Separated by Membrane Ultrafiltration. Folia Microbiologica 55(4): 386-389, 2010.

Stsiapanava, A., Dohnalek, J., Gavira, J. A., Kuty, M., Koudelakova, T., Damborsky, J., Kuta Smatanova, I. Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. Acta Crystallogr. D66: 962-969, 2010. PDB: 3fbw, 3g9x and 3fwh

Skalova, T., Duskova, J., Hasek, J., Kolenko, P., Stepankova, A., Dohnalek, J. Alternative polymer precipitants for protein crystallization. J. Appl. Crystallogr., 43: 737-742, 2010.