Gustavo Fuertes Vives

Gustavo Fuertes Vives

Junior Researcher and Head of Program “Dynamics of Biological Processes”

Gustavo Fuertes Vives works at the Institute of Biotechnnology CAS, where he focuses on the structural dynamics of photocontrolled DNA-binding proteins, principally EL222.

Gustavo applies integrative structural and chemical biology approaches for tackling outstanding biomolecular systems, like intrinsically disordered proteins, membrane proteins and protein/DNA complexes. Structure-function relationships of proteins are unveiled by the use of multiple molecular biology and biophysical techniques, mainly infrared and fluorescence spectroscopy and small-angle X-ray scattering. In parallel, Dr. Fuertes labels proteins with optical and vibrational probes for the acquisition of site-specific structural and dynamical information.

 

Recent publications

2020

Andrikopoulos P. C.; Liu Y.; Picchiotti A.; Lenngren N.; Kloz M.; Chaudhari A. S.; Precek M.; Rebarz M.; Andreasson J.; Hajdu J.; Schneider B.; Fuertes G.; Femtosecond-to-Nanosecond Dynamics of Flavin Mononucleotide Monitored by Stimulated Raman Spectroscopy and Simulations, PCCP 2020, https://doi.org/10.1039/C9CP04918E

2019

Fuertes, G., Nevola, L., and Esteban-Martin, S. (2019). Perspectives on drug discovery strategies based on IDPs in Intrinsically Disordered Proteins: Dynamics, Binding, and Function, Academic Press, pp. 275-327. https://doi.org/10.1016/B978-0-12-816348-1.00009-0

2018

Fuertes, G., Banterle, N., Ruff, K.M., Chowdhury, A., Pappu, R.V., Svergun, D.I., and Lemke,E.A (2018). Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water.” Science 361 (6405). https://doi.org/10.1126/science.aau8230

2017

Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, et al. Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A. 2017;114(31):E6342-E51. Epub 2017/07/19. doi: 10.1073/pnas.1704692114.

2016

Crosas E, Castellvi A, Crespo I, Fulla D, Gil-Ortiz F, Fuertes G, et al. Uridine as a new scavenger for synchrotron-based structural biology techniques. J Synchrotron Radiat. 2017;24(Pt 1):53-62. Epub 2016/12/24. doi: 10.1107/S1600577516018452.

2015

Mercadante D, Milles S, Fuertes G, Svergun DI, Lemke EA, Grater F. Kirkwood-Buff Approach Rescues Overcollapse of a Disordered Protein in Canonical Protein Force Fields. J Phys Chem B. 2015;119(25):7975-84. Epub 2015/06/02. doi: 10.1021/acs.jpcb.5b03440.

2014

Tyagi S, VanDelinder V, Banterle N, Fuertes G, Milles S, Agez M, et al. Continuous throughput and long-term observation of single-molecule FRET without immobilization. Nat Methods. 2014;11(3):297-300. Epub 2014/01/21. doi: 10.1038/nmeth.2809.

2011

Fuertes G, Sanchez-Munoz OL, Pedrueza E, Abderrafi K, Salgado J, Jimenez E. Switchable bactericidal effects from novel silica-coated silver nanoparticles mediated by light irradiation. Langmuir. 2011;27(6):2826-33. Epub 2011/02/11. doi: 10.1021/la1045282.

2010

Fuertes G, Gimenez D, Esteban-Martin S, Garcia-Saez A, Sanchez O, Salgado J. Role of membrane lipids for the activity of pore forming peptides and proteins. Adv Exp Med Biol. 2010;677:31-55. Epub 2010/08/07. doi: 10.1007/978-1-4419-6327-7_4.

Fuertes G, Garcia-Saez AJ, Esteban-Martin S, Gimenez D, Sanchez-Munoz OL, Schwille P, et al. Pores formed by Baxalpha5 relax to a smaller size and keep at equilibrium. Biophys J. 2010;99(9):2917-25. Epub 2010/11/04. doi: 10.1016/j.bpj.2010.08.068.