Ph.D. Positions - Structural Biology of Signaling Proteins

Ph.D. Positions - Structural Biology of Signaling Proteins

PhD project: Molecular mechanisms of 14-3-3 proteins in signaling complexes

14-3-3 proteins have the ability of binding the functionally different signal proteins, including kinases, phosphatases and transmembrane receptors by changing their function. Through the functional modulation of a wide range of binding partners, 14-3-3 proteins are involved in many important physiological and pathophysiological processes, including cell cycle regulation, metabolism control, apoptosis, and control of gene transcription. The scope of this PhD project lies in the field of structural biology/biophysical chemistry.

The main focus of this project will be on elucidation of mechanisms by which the 14-3-3 proteins regulate selected binding partners using a wide range of biophysical approaches. The work plan of the project contains preparation of recombinant proteins, biochemical and biophysical characterization of protein-protein interactions, and structural characterization of selected protein complexes, using protein crystallography, small angle x-ray scattering (SAXS) and fluorescence spectroscopy. The research will be performed in close cooperation with the Faculty of Science, Charles University.

More info at laboratory website:


Successful candidate should enjoy working in the field of structural biology/biophysics/physical chemistry, be curious and has the courage to try out new things. Required is M.Sc. degree or those expecting to obtain their degree this year (fields: Chemistry, Biochemistry, Molecular Biology or similar). The knowledge of molecular biology, protein expression and purification is a strong advantage. Good knowledge of English is required.

We offer

Friendly, motivating environment, part-time/full-time contract for 3 years with the possibility of renewal, 5 weeks of vacation, subsidized lunch.



RNDr. Veronika Obšilová, Ph.D.