Epigenetika buněčného jádra

Epigenetika buněčného jádra

Prof., RNDr. Pavel Hozák, DrSc. — Vedoucí projektu

O nás

Projekt se zabývá studiem dynamiky chromozomů v průběhu tvorby pohlavních buněk u dvou eukaryotických modelů (C. elegans, M. musculus).

Hlavním záměrem pracovní skupiny je studium proteinů, které se účastní rané fáze meiózy a to v průběhu párování a synapse homologních chromozomů. Identifikace nových faktorů, které mají vliv na meiózu při gametogeneze, patří mezi důležité a aktuální cíle, neboť chromozomální dynamika a možné komplikace během meiotického dělení jsou stále popsány nedostatečně. Přitom nepřesnosti během meiotické dělení způsobují chromozomální aberace, které vedou ke vzniku genetických poruch nebo k mužské neplodnosti.

Publikace

2017

Dong, L. F., Kovarova, J., Bajzikova, M., Bezawork-Geleta, A., Svec, D., Endaya, B., Sachaphibulkij, K., Coelho, A. R., Sebkova, N., Ruzickova, A., Tan, A. S., Kluckova, K., Judasova, K., Zamecnikova, K., Rychtarcikova, Z., Gopalan, V., Sobol, M., Yan, B., Pattnaik, B., Bhatraju, N., Truksa, J., Stopka, P., Hozak, P., Lam, A., Sedlacek, R., Oliveira, P. J., Kubista, M., Agrawal, A., Dvorakova-Hortova, K., Rohlena, J., Berridge, M. V., Neuzil, J. Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells. eLife, 6:e22187, 2017. doi: 10.7554/eLife.22187. ISSN 2050-084X.

Dráberová E, Sulimenko V, Vinopal S, Sulimenko T, Sládková V, D'Agostino L, Sobol M, Hozák P, Křen L, Katsetos CD, Dráber P: Differential expression of human γ-tubulin isotypes during neuronal development and oxidative stress points to a γ-tubulin-2 prosurvival function FASEB JOURNAL 2017 31(5): 1828-1846.

Fabrik I, Link M, Putzova D, Plzakova L, Lubovska Z, Philimonenko V, Pavkova I, Rehulka P, Krocova Z, Hozak P, Santic M, Stulik J: The early dendritic cell signaling induced by virulent Francisellatularensis strain occurs in phases and involves the activation of ERKs and p38 in the later stage. Mol Cell Proteomics 2017.

Fišerová J, Efenberková M, Sieger T, Maninová M, Uhlířová J, Hozák P: Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data. J Cell Sci 2017 130(12): 2066-2077.

Morales J, Sobol M, Rodriguez-Zapata LC, Hozak P, Castano E: Aromatic amino acids and their relevance in the specificity of the PH domain. J Mol Recognit 2017 30(12).

Pastorek L, Venit T, Hozák P: Holography microscopy as an artifact-free alternative to phase-contrast. Histochem Cell Biol 2017.

Pavkova I, Kopeckova M, Klimentova J, Schmidt M, Sheshko V, Sobol M, Zakova J, Hozak P, Stulik J: The Multiple Localized Glyceraldehyde-3-Phosphate Dehydrogenase Contributes to the Attenuation of the Francisella tularensis dsbA Deletion Mutant. Front Cell Infect Microbiol 2017 7: 503.

Rodriguez-Corona U, Pereira-Santana A, Sobol M, Rodriguez-Zapata LC, Hozak P, Castano E: Novel Ribonuclease Activity Differs between Fibrillarins from Arabidopsis thaliana. Front Plant Sci 2017 8: 1878.

Rohlenova K, Sachaphibulkij K, Stursa J, Bezawork-Geleta A, Blecha J, Endaya B, Werner L, Cerny J, Zobalova R, Goodwin J, Spacek T, Alizadeh Pesdar E, Yan B, Nguyen MN, Vondrusova M, Sobol M, Jezek P, Hozak P, Truksa J, Rohlena J, Dong LF, Neuzil J: Selective Disruption of Respiratory Supercomplexes as a New Strategy to Suppress Her2(high) Breast Cancer. Antioxid Redox Signal 2017 26(2): 84-103.

Ulicna L, Kalendova A, Kalasova I, Vacik T, Hozák P: PIP2 epigenetically represses rRNA genes transcription interacting with PHF8. Biochim Biophys Acta 2017 1863(3): 266-275.

2016

Kalasova I, Fáberová V, Kalendová A, Yildirim S, Uličná L, Venit T, Hozák P: Tools for visualization of phosphoinositides in the cell nucleus. Histochem Cell Biol 2016 145(4): 485-96.

Nebesářová J, Hozák P, Frank L, Štěpan P, Vancová M: The cutting of ultrathin sections with the thickness less than 20 nm from biological specimens embedded in resin blocks. Microsc Res Tech 2016 79(6): 512-7

Pasdar EA, Smits M, Stapelberg M, Bajzikova M, Stantic M, Goodwin J, Yan B, Stursa J, Kovarova J, Sachaphibulkij K, Bezawork-Geleta A, Sobol M, Filimonenko A, Tomasetti M, Zobalova R, Hozak P, Dong LF, Neuzil J: Correction: Characterisation of Mesothelioma-Initiating Cells and Their Susceptibility to Anti-Cancer Agents. PLoS One 2016 11(5): e0156012.

Tomasetti M, Monaco F, Manzella N, Rohlena J, Rohlenova K, Staffolani S, Gaetani S, Ciarapica V, Amati M, Bracci M, Valentino M, Goodwin J, Nguyen M, Truksa J, Sobol M, Hozak P, Dong LF, Santarelli L, Neuzil J: MicroRNA-126 induces autophagy by altering cell metabolism in malignant mesothelioma. Oncotarget 2016 7(24): 36338-36352.

Venit T, Kalendová A, Petr M, Dzijak R, Pastorek L, Rohožková J, Malohlava J, Hozák P: Nuclear myosin I regulates cell membrane tension. Sci Rep 2016 6: 30864.

Vrbacký M, Kovalčíková J, Chawengsaksophak K, Beck IM, Mráček T, Nůsková H, Sedmera D, Papoušek F, Kolář F, Sobol M, Hozák P, Sedlacek R, Houštěk J: Knockout of Tmem70 alters biogenesis of ATP synthase and leads to embryonal lethality in mice. Hum Mol Genet 2016.

Zhironkina OA, Kurchashova SY, Pozharskaia VA, Cherepanynets VD, Strelkova OS, Hozak P, Kireev II: Mechanisms of nuclear lamina growth in interphase. Histochem Cell Biol 2016 145(4): 419-32.

2015

Dráberová E, D'Agostino L, Caracciolo V, Sládková V, Sulimenko T, Sulimenko V, Sobol M, Maounis NF, Tzelepis E, Mahera E, Křen L, Legido A, Giordano A, Mörk S, Hozák P, Dráber P, Katsetos CD: Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol 2015 74(7): 723-42.

Kitamura H, Matsumori H, Kalendova A, Hozak P, Goldberg IG, Nakao M, Saitoh N, Harata M: The actin family protein ARP6 contributes to the structure and the function of the nucleolus. Biochem Biophys Res Commun 2015 464(2): 554-60.

Kosi N, Alić I, Kolačević M, Vrsaljko N, Jovanov Milošević N, Sobol M, Philimonenko A, Hozák P, Gajović S, Pochet R, Mitrečić D: Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain. Brain Res 2015 1597: 65-76.

Loizeau D, Jurkiewicz P, Aydogan G, Philimonenko AA, Mahfoudhi S, Hozák P, Maroto A, Couthon-Gourvès H, Jaffrès PA, Deschamps L, Giamarchi P, Hof M: Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics. Colloids Surf B Biointerfaces 2015 136: 192-200.

Loza-Muller L, Rodríguez-Corona U, Sobol M, Rodríguez-Zapata LC, Hozak P, Castano E: Fibrillarin methylates H2A in RNA polymerase I trans-active promoters in Brassica oleracea. Front Plant Sci 2015 6: 976.

Marášek P, Dzijak R, Studenyak I, Fišerová J, Uličná L, Novák P, Hozák P: Paxillin-dependent regulation of IGF2 and H19 gene cluster expression. J Cell Sci 2015 128(16): 3106-16

Margaryan H, Dorosh A, Capkova J, Manaskova-Postlerova P, Philimonenko A, Hozak P, Peknicova J: Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm. Reprod Biol Endocrinol 2015 13: 15.

Pasdar EA, Smits M, Stapelberg M, Bajzikova M, Stantic M, Goodwin J, Yan B, Stursa J, Kovarova J, Sachaphibulkij K, Bezawork-Geleta A, Sobol M, Filimonenko A, Tomasetti M, Zobalova R, Hozak P, Dong LF, Neuzil J: Characterisation of mesothelioma-initiating cells and their susceptibility to anti-cancer agents. PLoS One 2015 10(5): e0119549.

Rodriguez-Corona U, Sobol M, Rodriguez-Zapata LC, Hozak P, Castano E: Fibrillarin from Archaea to human. Biol Cell 2015 107(6): 159-74.

Tan AS, Baty JW, Dong LF, Bezawork-Geleta A, Endaya B, Goodwin J, Bajzikova M, Kovarova J, Peterka M, Yan B, Pesdar EA, Sobol M, Filimonenko A, Stuart S, Vondrusova M, Kluckova K, Sachaphibulkij K, Rohlena J, Hozak P, Truksa J, Eccles D, Haupt LM, Griffiths LR, Neuzil J, Berridge MV: Mitochondrial genome acquisition restores respiratory function and tumorigenic potential of cancer cells without mitochondrial DNA. Cell Metab 2015 21(1): 81-94.

2014

Härtlová A, Link M, Balounová J, Benešová M, Resch U, Strasková A, Sobol M, Philimonenko A, Hozák P, Krocová Z, Gekara N, Filipp D, Stulík J: Quantitative Proteomics Analysis of Macrophage-derived Lipid Rafts Reveals Induction of Autophagy Pathway at the Early Time of Francisella tularensis LVS infection. J Proteome Res 2014 13(2): 796-804.

Kalendová A, Kalasová I, Yamazaki S, Uličná L, Harata M, Hozák P: Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block. Histochem Cell Biol 2014 142(2): 139-52.

Philimonenko VV, Philimonenko AA, Šloufová I, Hrubý M, Novotný F, Halbhuber Z, Krivjanská M, Nebesářová J, Šlouf M, Hozák P: Simultaneous detection of multiple targets for ultrastructural immunocytochemistry. Histochem Cell Biol 2014 141(3): 229-39.

2013

Roth J, Debbage P, Hozák P: In focus in Prague: intermediate filaments. Histochem Cell Biol 2013 140(1): 1-2.

Sobol M, Yildirim S, Philimonenko VV, Marášek P, Castaño E, Hozák P: UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity. Nucleus 2013 4(6): 478-86.

Venit T, Dzijak R, Kalendová A, Kahle M, Rohožková J, Schmidt V, Rülicke T, Rathkolb B, Hans W, Bohla A, Eickelberg O, Stoeger T, Wolf E, Yildirim AÖ, Gailus-Durner V, Fuchs H, de Angelis MH, Hozák P: Mouse nuclear Myosin I knock-out shows interchangeability and redundancy of Myosin isoforms in the cell nucleus. PLoS One 2013 8(4): e61406

Yildirim S, Castano E, Sobol M, Philimonenko VV, Dzijak R, Venit T, Hozák P: Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription. J Cell Sci 2013 126(Pt 12): 2730-9.

Tým

Prof., RNDr.
Pavel Hozák, DrSc.

Vedoucí skupiny Struktury buněčného jádra v genové expresi

pavel.hozak@img.cas.cz
+420325873104