Role metabolismu, signálních molekul a buněčných struktur při stárnutí, stresu a adaptaci

RNDr. Libuše Váchová, CSc. — Vedoucí projektu

O nás

The project focuses on molecular processes and regulatory mechanisms involved in the adaptation, aging and cell death of yeast populations and in the response of populations to different stresses. The project focuses mainly on multicelluar populations such as biofilms and colonies that are capable of differentiation.

The main topics currently studied included identification of specific metaboli processes and defense strategies important for long-term developoment of colonies and biofilms and different roles of celluar organeles (e. g. mitochondria, peroxisomes and autophagosomes). Different approaches are used in the studies that include, in addition to a wide spectrum of methods of microbiology, biochemistry and cell and molecular biology, unique techniques developed in the laboratory for investigating yeast populations. These techniques include, for example, specific approaches of two photon confocal microscopy. The research in the project takes advantage from of the close collaboration with project 2. 1. 5., which also includes collaboration with the G. Gilfillan group in Oslo, Norway of Czech-Norwegian grant funding.



The expertise that we offer involves physiology and cell biology of yeast populations, including an analysis of selected stress defense processes. We also offer cooperation in the field of immobilized yeast populations covered by our patent "Modification method of detection yeast strain" No 305223, which enables detection of different chemicals that have been applied in Disposable kit for detection of the content of cupric (Cu2+) ions in drinking waters and industrial wastewaters developed with LentiKat's a.s.




Wilkinson D., Maršíková J., Hlaváček O., Gilfillan GD., Ježková E., Aaløkken R., Váchová L., Palková Z. (2018) Transcriptome remodeling of differentiated cells during chronological ageing of yeast colonies: New insights into metabolic differentiation. Oxidative Medicine and Cellular Longevity, Volume 2018, Article ID 4932905

Vachova L., Palkova Z. (2018) How structured yeast multicellular communities live, age and die? FEMS Yeast Research 18, 2018, foy033, 1-9

Segorbe, D., Wilkinson D., Mizeranschi A., Hughes T., Aaløkken R., Vachova L., Palkova Z., D. Gilfillan G.D. (2018) An optimized FAIRE procedure for low cell numbers in yeast. Yeast 2018: 1-6 doi: 10.1002/yea.3316

Aouacheria A, Cunningham, KW., Hardwick, JM., Palková, Z., Powers, T., Severin, FF., Váchová, L., Comment on “Sterilizing immunity in the lung relies on targeting fungal apoptosis-like programmed cell death”, Science 360 (6395): eaar6910, DOI: 10.1126/science.aar6910

Van Nguyen P., Hlavacek O., Marsikova J., Vachova L., Palkova Z. (2018) Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity. PLoS genetics,


Hovsepian J., Defenouillère Q., Albanèse V., Váchová L., Garcia C., Palková Z., Léon S. (2017) Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis. J. Cell Biol. 216(6): 1811-1831

Maršíková J., Wilkinson D., Hlaváček O., Gilfillan G., Mizeranschi A., Hughes T., Begany M., Rešetárová S., Váchová L., Palková Z. (2017) Metabolic differentiation of surface and invasive cells of yeast colony biofilms revealed by gene expression profiling. BMC Genomics (2017) 18:814, DOI 10.1186/s12864-017-4214-4


Palkova Z, Vachova L (2016) Yeast cell differentiation: Lessons from pathogenic and non-pathogenic yeasts. Semin Cell Dev Biol. 57: 110-9.

Podholova K, Plocek V, Resetarova S, Kucerova H, Hlavacek O, Vachova L, Palkova Z (2016) Divergent branches of mitochondrial signaling regulate specific genes and the viability of specialized cell types of differentiated yeast colonies. Oncotarget 7: 15299-314.

Kuznetsov E., Váchová L., Palková Z. (2016) Cellular localization of Sun4p and its interaction with proteins in the yeast birth scar. Cell Cycle 15(14): 1898-1907

Palková Z., Váchová L. (2016) Mitochondria in aging cell differentiation. Aging-US 8(7): 1287-1288

Klionsky D.J. et al (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12(1): 1-222, DOI:10.1080/15548627.2015.1100356


Vopálenská I., Váchová L., Palková Z. (2015) New biosensor for detection of copper ions in water based on immobilized genetically modified yeast cells. Biosensors and Bioelectronics 72: 160–167

Čáp M., Váchová L, Palková Z. (2015) Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis. Cell Cycle 14: 21, 3488-3497


Palkova, Z, Wilkinson, D, Vachova, L (2014) Aging and differentiation in yeast populations: Elders with different properties and functions. FEMS Yeast Res 14: 96-108

Palková Z., Wilkinson D., Váchová L. (2014) Aging and differentiation in yeast populations: Elders with different properties and functions. FEMS Yeast Res. 14 (1): 96-108

Šťovíček V., Váchová L., Begany M., Wilkinson D., PalkováZ. (2014) Global changes in gene expression associated with phenotypic switching of wild yeast. BMC Genomics 15: Art. No. 136, DOI: 10.1186/1471-2164-15-136


Paiva S, Strachotová D, Kučerová H, Hlaváček O, Mota S, Casal M, Palková Z, Váchová L. The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies. Biochem J. 2013 Sep 15;454(3):551-8.

Kuznetsov E,, Kučerová H,, Váchová L,, Palková Z, (2013) SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level. PLoS One. 2013 Sep 11;8(9):e73882. doi: 10.1371/journal.pone.0073882

Paiva S., Strachotová D., Kučerová H., Hlaváček O., Mota S., Casal M., Palková Z., Váchová L. (2013) The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies. Biochem. J. 454 (3): 551-558

Váchová L., Hatáková L., Čáp M., Pokorná M., Palková Z. (2013) Rapidly Developing Yeast Microcolonies Differentiate in a Similar Way to Aging Giant Colonies. Oxidative Medicine and Cellular Longevity, Article Number: UNSP 102485, doi:  10.1155/2013/102485


Cottier F, Raymond M, Kurzai O, Bolstad M, Leewattanapasuk W, Jiménez-López C, Lorenz MC, Sanglard D, Váchová L, Pavelka N, Palková Z, Mühlschlegel FA.: The bZIP transcription factor Rca1p is a central regulator of a novel CO2 sensing pathway in yeast. PLoS Pathogens 2012; 8(1): e1002485

Čáp M, Štěpánek L, Harant K, Váchová L, Palková Z.: Cell differentiation within a yeast colony: metabolic and regulatory parallels with a tumor-affected organism. Molecular Cell 2012; 46: 436-48.


Váchová L, Šťovíček V, Hlaváček O, Chernyavskiy O, Štěpánek L, Kubínová L, Palková Z.: Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies. Journal of Cell Biology 2011; 194: 679-87


Libuše Váchová, CSc.

Vedoucí skupiny Role metabolismu, signálních molekul a buněčných struktur při stárnutí, stresu a adaptaci
+420325873926, +420325873933

Otakar Hlaváček, Ph.D.

Stanislava Rešetárová, Ph.D.