Molekulární a buněčná biologie kvasinkových populací, interakce, signalizace a diferenciace

Molekulární a buněčná biologie kvasinkových populací, interakce, signalizace a diferenciace

Prof. RNDr. Zdena Palková, CSc.

Prof. RNDr. Zdena Palková, CSc. — Vedoucí projektu

O nás

Výzkum naší skupiny je zaměřen na studium vývoje a diferenciace kvasinkových kolonií a biofilmů. Projekt je řešen v těsné spolupráci s Laboratoří buněčné biologie Dr. Libuše Váchové (MBÚ, AV ČR).

Kvasinkové buňky rostoucí na pevném povrchu vytvářejí mnohobuněčné struktury, kolonie a biofilmy, s charakteristickou organizovanou morfologií. Kvasinkové buňky v průběhu vývoje kolonie diferencují a vytvářejí specificky lokalizované buněčné subpopulace se specializovanou rolí v rámci struktury kolonie. Kolonie kvasinek se tedy chovají jako jednoduché mnohobuněčné organizmy, jejichž buňky navzájem komunikují, synchronizují svůj vývoj a diferencují do primitivních „tkání“. Buněčná diferenciace často přispívá k dlouhověkosti populace celé kolonie. Současný výzkum probíhá v následujících hlavních směrech:

  1. Vývoj a diferenciace hladkých kolonií tvořených laboratorními a domestikovaným kmeny Saccharomyces cerevisiae
  2. Vývoj a diferenciace koloniových biofilmů tvořených přírodními kmeny Saccharomyces cerevisiae

V poslední době byl zahájen nový výzkumný směr zaměřený na identifikaci vybraných diferenciačních procesů v koloniích a biofilmech patogenních kvasinek a během jejich interakce s buňkami hostitele.

Více informací ZDE

Aktuality

Publikace

2018

Wilkinson D., Maršíková J., Hlaváček O., Gilfillan GD., Ježková E., Aaløkken R., Váchová L., Palková Z. (2018) Transcriptome remodeling of differentiated cells during chronological ageing of yeast colonies: New insights into metabolic differentiation. Oxidative Medicine and Cellular Longevity, Volume 2018, Article ID 4932905

Wilkinson D., Váchová L., Hlaváček O., Maršíková J., Gilfillan G.D., Palková Z. (2018) Long non-coding RNAs in yeast cells and differentiated subpopulations of yeast colonies and biofilms. Oxidative Medicine and Cellular Longevity, Volume 2018, Article ID 4950591, https://doi.org/10.1155/2018/4950591

Vachova L., Palkova Z. (2018) How structured yeast multicellular communities live, age and die? FEMS Yeast Research 18, 2018, foy033, 1-9

Segorbe, D., Wilkinson D., Mizeranschi A., Hughes T., Aaløkken R., Vachova L., Palkova Z., D. Gilfillan G.D. (2018) An optimized FAIRE procedure for low cell numbers in yeast. Yeast 2018: 1-6 doi: 10.1002/yea.3316

Aouacheria A, Cunningham, KW., Hardwick, JM., Palková, Z., Powers, T., Severin, FF., Váchová, L., Comment on “Sterilizing immunity in the lung relies on targeting fungal apoptosis-like programmed cell death”, Science 360 (6395): eaar6910, DOI: 10.1126/science.aar6910

Van Nguyen P., Hlavacek O., Marsikova J., Vachova L., Palkova Z. (2018) Cyc8p and Tup1p transcription regulators antagonistically regulate Flo11p expression and complexity. PLoS genetics, https://doi.org/10.1371/journal.pgen.1007495

2017

Hovsepian J., Defenouillère Q., Albanèse V., Váchová L., Garcia C., Palková Z., Léon S. (2017) Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis. J. Cell Biol. 216(6): 1811-1831

Maršíková J., Wilkinson D., Hlaváček O., Gilfillan G., Mizeranschi A., Hughes T., Begany M., Rešetárová S., Váchová L., Palková Z. (2017) Metabolic differentiation of surface and invasive cells of yeast colony biofilms revealed by gene expression profiling. BMC Genomics (2017) 18:814, DOI 10.1186/s12864-017-4214-4

2016

Palkova Z, Vachova L (2016) Yeast cell differentiation: Lessons from pathogenic and non-pathogenic yeasts. Semin Cell Dev Biol. 57: 110-9.

Podholova K, Plocek V, Resetarova S, Kucerova H, Hlavacek O, Vachova L, Palkova Z (2016) Divergent branches of mitochondrial signaling regulate specific genes and the viability of specialized cell types of differentiated yeast colonies. Oncotarget 7: 15299-314.

Kuznetsov E., Váchová L., Palková Z. (2016) Cellular localization of Sun4p and its interaction with proteins in the yeast birth scar. Cell Cycle 15(14): 1898-1907

Palková Z., Váchová L. (2016) Mitochondria in aging cell differentiation. Aging-US 8(7): 1287-1288

Klionsky D.J. et al (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12(1): 1-222, DOI:10.1080/15548627.2015.1100356

2015

Vopálenská I., Váchová L., Palková Z. (2015) New biosensor for detection of copper ions in water based on immobilized genetically modified yeast cells. Biosensors and Bioelectronics 72: 160–167

Čáp M., Váchová L, Palková Z. (2015) Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis. Cell Cycle 14: 21, 3488-3497

2014

Palková Z., Wilkinson D., Váchová L. (2014) Aging and differentiation in yeast populations: Elders with different properties and functions. FEMS Yeast Res. 14 (1): 96-108

Šťovíček V., Váchová L., Begany M., Wilkinson D., PalkováZ. (2014) Global changes in gene expression associated with phenotypic switching of wild yeast. BMC Genomics 15: Art. No. 136, DOI: 10.1186/1471-2164-15-136

2013

Kuznetsov E,, Kučerová H,, Váchová L,, Palková Z, (2013) SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level. PLoS One. 2013 Sep 11;8(9):e73882. doi: 10.1371/journal.pone.0073882

Paiva S., Strachotová D., Kučerová H., Hlaváček O., Mota S., Casal M., Palková Z., Váchová L. (2013) The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies. Biochem. J. 454 (3): 551-558

Váchová L., Hatáková L., Čáp M., Pokorná M., Palková Z. (2013) Rapidly Developing Yeast Microcolonies Differentiate in a Similar Way to Aging Giant Colonies. Oxidative Medicine and Cellular Longevity, Article Number: UNSP 102485, doi:  10.1155/2013/102485

2012

Váchová L., Čáp M., Palková Z. (2012) Yeast Colonies: A Model for Studies of Aging, Environmental Adaptation, and Longevity. Oxidative Med. Cell. Longev. Article Number: 601836   DOI: 10.1155/2012/601836

Šťovíček V., Váchová L., Palková Z. (2012) Yeast biofilm colony as an orchestrated multicellular organism. Commun Integr Biol 5: 203-205

Pineda-Rodó A., Váchová L., Palková Z. (2012) In vivo determination of organellar pH using a universal wavelength-based confocal microscopy approach, PLOS One 7: e33229, doi:10.1371/journal.pone. 0033229

Palkova Z., Vachova L. (2012) Communication and differentiation in the development of yeast colonies, In: Biocommunication in Fungi, Edited by Guenther Witzany, Springer 2012, pp 141-154

Čáp M., Váchová L., Palková Z. (2012) Reactive oxygen species in the signaling and adaptation of multicellular microbial communities. Oxidative Med. Cell. Longev. Volume 2012, Article ID 976753, doi:10.1155/2012/976753

Čáp M., Štěpánek L., Harant K., Vachová L., Palková Z. (2012) Cell differentiation within a yeast colony: Metabolic and regulatory parallels with a tumor-affected organism. Molecular Cell 46: 436-448

Cottier F., Raymond M., Kurzai O., Bolstad M., Leewattanapasuk W., Jiménez-López C. , Lorenz MC., Sanglard D., Váchová L., Pavelka N.,  Palková Z., Mühlschlegel FA. (2012). The bZIP transcription factor Rca1p is a central regulator of a novel CO2 sensing pathway in yeast. PLOS Pathogen 8: e1002485,   DOI: 10.1371/journal.ppat.1002485

Strachotová D., Holoubek A., Kučerová H., Benda A., Humpolíčková J., Váchová L., Palková Z. (2012) Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM). BBA-Biomembranes 1818: 2126-2134 

2011

Vachova L., Stovicek V., Hlavacek O., Chernyavskiy O., Stepanek L., Kubinova L., Palkova Z. (2011) Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies. J Cell Biol. 194: 679-87

Vachova L., Palkova Z. (2011) Aging and longevity of yeast colony populations: metabolic adaptation and differentiation. Biochem Soc Trans. 39: 1471-5

2010

Vopalenska I., Stovicek V., Janderova B., Vachova L., Palkova Z. (2010) Role of distinct dimorphic transitions in territory colonizing and formation of yeast colony architecture. Environ Microbiol 12: 264-77

Stovicek V., Vachova L., Kuthan M., Palkova Z. (2010) General factors important for the formation of structured biofilm-like yeast colonies. Fungal Genet Biol. 47: 1012-22

Cap M., Vachova L., Palkova Z. (2010) How to survive within a yeast colony: Change metabolism or cope with stress? Commun Integr Biol 3: 198-200

2009

Vachova L., Kucerova H., Devaux F., Ulehlova M., Palkova Z. (2009) Metabolic diversification of cells during the development of yeast colonies. Environ. Microbiol. 11: 494-504

Vachova L., Chernyavskiy O., Strachotová D., Bianchini P., Burdiková Z., Ferciková I., Kubinova L., Palkova Z. (2009) Architecture of developing multicellular yeast colony: spatio-temporal expression of Ato1p ammonium exporter. Environ Microbiol. 11: 1866-1877

Palkova Z., Vachova L., Gaskova D., Kucerova H. (2009) Synchronous plasma membrane electrochemical potential oscillations during yeast colony development and aging. Mol Membr Biol. 26: 228-35

Hlavacek O., Kucerova H., Harant K., Palková Z., Vachová L. (2009) Putative role for ABC multidrug exporters in yeast quorum sensing. FEBS Lett. 583: 1107-13

Cap M., Vachova L., Palkova Z. (2009) Yeast colony survival depends on metabolic adaptation and cell differentiation rather than on stress defense. J Biol Chem. 284: 32572-81

2007

Váchová L., Palková Z. (2007) Caspases in yeast apoptosis-like death: facts and artefacts. FEMS Yeast Res. 7: 12-21

Ricicova M., Kucerova H, Vachova L., Palkova Z. (2007) Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localization in patches. BBA-Biomembranes, 1768: 1170-8

2006

Palková Z., Váchová L. (2006) Life within a community: benefit to yeast long-term survival. FEMS Microbiol Rev 30: 806–824

2005

Vopalenska I., Hulkova M., Janderova B., Palkova Z. (2005) The morphology of Saccharomyces cerevisiae colonies is affected by cell adhesion and the budding pattern. Res Microbiol. 156: 921-31

Adamec T., Palkova Z., Velkova K., Stokrova J., Forstova J. (2005) Point mutation in calcium-binding domain of mouse polyomavirus VP1 protein does not prevent virus-like particle formation, but changes VP1 interactions with Saccharomyces cerevisiae cell structures. FEMS Yeast Res. 5: 331-40

Vachova L., Palkova Z. (2005) Physiological regulation of yeast cell death in multicellular colonies is triggered by ammonia. J Cell Biol. 169: 711-7

2004

Vachova L., Devaux F., Kucerova H., Ricicova M., Jacq C., Palkova Z. (2004) Sok2p transcription factor is involved in adaptive program relevant for long-term survival of Saccharomyces cerevisiae colonies. J Biol Chem. 279:  37973-81

Palková Z., Váchová L., Valer M., Preckel T. (2004) Single cell analysis of yeast, mammalian cells and fungal spores with a microfluidic pressure driven chip-based system. Cytometry, part A 59: 246-253

Palková Z. (2004) Multicellular microorganisms: Laboratory versus nature. EMBO Reports, 5: 470-476

2003

Ricicova M., Palkova Z. (2003) Comparative analyses of Saccharomyces cerevisiae RNAs using Agilent RNA 6000 Nano Assay and agarose gel electrophoresis. FEMS Yeast Res. 4: 119-22

Palková Z., Vachova L. (2003) Ammonia signalling in yeast colony formation. Int. Rev. Cytol., 225: 229 – 272, Invited review

Kuthan M., Devaux F., Janderová B., Slaninová I, Jacq C., Palková Z. (2003) Domestication of wild Saccharomyces cerevisiae is accompanied by changes in gene expression and colony morphology.  Mol. Microbiol.47: 745-754

Adamec T., Palkova Z., Forstova J. (2003) Mouse polyomavirus large T antigen inhibits cell growth and alters cell and colony morphology in Saccharomyces cerevisiae. FEBS Lett. 555:268-73

2002

Zikanova B., Kuthan M., Ricicova M., Forstova J., Palkova Z. (2002) Amino acids control ammonia pulses in yeast colonies. Biochem. Biophys. Res. Commun. 294: 962-967

Palkova Z., Devaux F., Ricicova M., Minarikova L., LeCrom S., Jacq C. (2002) Ammonia pulses and metabolic oscillations guide yeast colony development. Mol. Biol. Cell. 13: 3901-3914

2001

Minarikova L., Kuthan M., Ricicova M., Forstova J., Palkova Z. (2001) Differentiated gene expression in cells within yeast colonies.  Exp. Cell Res. 271: 296-304

2000

Palková Z., Adamec T., Lieb D., Stokrová J., Forstová J. (2000). Production of polyomavirus structural protein, VP1, in yeast cells and its interaction with cell structures. FEBS Lett. 478: 281-289

Palková Z., Forstová J. (2000). Yeast colonies synchronise their growth and development. J. Cell Sci. 113: 1923-1928

1997

Palková Z., Janderová B., Gabriel J., Zikánová B., Pospíšek M., Forstová J.(1997) Ammonia mediates communication between yeast colonies, Nature 390: 532-536

Tým

Prof. RNDr. Zdena Palková, CSc.

Prof. RNDr.
Zdena Palková, CSc.

Vedoucí výzkumné skupiny, členka Rady BIOCEV

zdena.palkova@natur.cuni.cz
+420325873933, +420325873929
Mgr. Martin Kuthan, Ph.D.

Mgr.
Martin Kuthan, Ph.D.

vědecký pracovník

martin.kuthan@natur.cuni.cz
+420325873928, +420325873926
RNDr. Tomáš Hendrych, Ph.D.

RNDr.
Tomáš Hendrych, Ph.D.

vědecký pracovník

tomas.hendrych@natur.cuni.cz
+420325873934, +420325873929
Derek Wilkinson, Ph.D.

Derek Wilkinson, Ph.D.

vědecký pracovník

derek.wilkinson@natur.cuni.cz
+420325873934, +420325873929

Mgr.
Irena Vopálenská, Ph.D.

vědecká pracovnice

irena.vopalenska@natur.cuni.cz
+420325873932, +420325873930
David Segorbe Luque, M.Sc., Ph.D.

David Segorbe Luque, M.Sc., Ph.D.

vědecký pracovník

segorbed@natur.cuni.cz
+420325873934, +420325873926

Mgr.
Vítězslav Plocek

student Ph.D.

vitezslav.plocek@natur.cuni.cz
+420325873932, +420325873929
Mgr. Jana Maršíková

Mgr.
Jana Maršíková

studentka Ph.D.

jana.rusinova@natur.cuni.cz
+420325873932, +420325873926
Mgr. Kristýna Podholová

Mgr.
Kristýna Podholová

studentka Ph.D.

kristyna.podholova@natur.cuni.cz
+420325873931, +420325873929
Mgr. Pavla Novotná

Mgr.
Pavla Novotná

studentka Ph.D.

pavla.novotna@natur.cuni.cz
+420325873926
Hana  Žďárská

Hana Žďárská

technik

hana.zdarska@natur.cuni.cz
+420325873929
Ing. Miroslava Úlehlová

Ing.
Miroslava Úlehlová

technik VŠ

mirule@natur.cuni.cz
+420325873927
Mgr. Michal Čáp, Ph.D.

Mgr.
Michal Čáp, Ph.D.

vědecký pracovník

michal.cap@natur.cuni.cz.cz
+420325873928, +420325873930
Phu Van Nguyen, M.Sc.

Phu Van Nguyen, M.Sc.

Student Ph.D

nguyenvp@natur.cuni.cz
+420325873932, +420325873930