Zobrazovací metody

Zobrazovací metody

Mgr. Aleš Benda, Ph.D.

Mgr. Aleš Benda, Ph.D. — Vedoucí laboratoře

O nás

Servisní laboratoř Zobrazovací metody pokrývá tři základní oblasti – optickou (fluorescenční) mikroskopii, elektronovou mikroskopii a průtokovou cytometrii. Nabízíme našim uživatelům nejen zacvičení a přístup k pokročilým přístrojům, ale i konzultační služby týkající se optimálního nastavení podmínek experimentu, přípravy vzorku, sběru dat, případně analýzy a interpretace získaných výsledků. Organizujeme také mikroskopické kurzy.

 

Více informací naleznete zde

 

Napojení na národní a mezinárodní výzkumné infrastruktury a konsorcia


Czech-Bioimaging

Servisní laboratoř Zobrazovací metody je součástí infrastruktury Czech-Bioimaging, která poskytuje otevřený přístup k široké škále zobrazovacích technik a expertíze nejen pro vědce v České republice. 

Provoz servisní laboratoře je částečně hrazen z prostředků programu pro velké výzkumné infrastruktury Ministerstva školství, mládeže a tělovýchovy v rámci projektu „Národní infrastruktura pro biologické a medicínské zobrazování (Czech BioImaging – LM2015062)“.

Jak získat přístup do Cz-BI

Aplikační formulář pro otevřený přístup do CzBI

Euro-Bioimaging

Servisní laboratoř Zobrazovací metody tvoří část pražského uzlu celoevropské zobrazovací infrastruktury Euro-BioImaging, která poskytuje otevřený přístup, servis a školení k široké škále biologických a medicínskych zobrazovacích metod.

Jak získat přístup do EUBI 

Aplikační formulář pro otevřený přístup do EUBI

Poděkování


MIKROSKOPIE

We acknowledge Imaging Methods Core Facility at BIOCEV, institution supported by the MEYS CR (Large RI Project LM2018129 Czech-BioImaging) and ERDF (project No. CZ.02.1.01/0.0/0.0/16_013/0001775) for their support with obtaining imaging data presented in this paper.


PRŮTOKOVÁ CYTOMETRIE

We acknowledge the Imaging Methods Core Facility at BIOCEV for their support with obtaining flow cytometry data presented in this paper.

 

Užitečné odkazy

Aktuality

Akce

Služby

Naše servisní laboratoř poskytuje služby v oblasti optické (fluorescenční) mikroskopie, elektronové mikroskopie a průtokové cytometrie s možnou pomocí při následné analýze dat

Jak nás kontaktovat

Informace pro nové uživatele:

  1. Kontaktujte nás
    • Chcete-li využít našich služeb, neváhejte kontaktovat kteréhokoliv člena IMCF týmu telefonicky, nebo e-mailem. 

  2. Dohodněte si s námi setkání
    • V průběhu osobního setkání se členem našeho týmu, prodiskutujete váš projekt a společně probereme služby IMCF, které vám můžeme nabídnout.
    • Během prvního setkání proběhne také vaše registrace do databáze Czech BioImaging Open Access.

  3. Registrujte se do našeho rezervačního systému
    • Prostřednictvím systému budete informováni o dostupnosti přístrojů a zároveň budete schopni měnit rezervace dle obsazenosti a vašich možností. Postup jak se registrovat do rezervačního systému naleznete zde.

  4. Účastněte se teoretického i praktického tréninku
    • Během školení vás seznámíme se základními principy měření a ovládání mikroskopu/cytometru.

  5. Získejte certifikát
    • Po praktickém tréninku obdržíte certifikát, který vám umožní měření na vybraném přístroji bez naší asistence.

  6. Samostatné měření
    • Nyní můžete začít se svým experimentem. Pokud si s čímkoliv během měření nevíte rady, neváhejte se na nás obrátit s žádostí o pomoc.

 

Rezervační systém

Služby

01

Optická (fluorescenční) mikroskopie

02

Elektronová mikroskopie

03

Průtoková cytomerie

04

Analýza dat

Vybavení

Průtoková cytometrie

BD LSRFortessaTM SORP

BD LSRFortessaTM SORP

  • Multicolour flow cytometry analysis
  • 5 spatially separated lasers
  • 18 fluorescent detectors
  • Sample loading options:
    • 5ml tubes
    • 96-, 384-well plates

More info

BD FACS Aria Fusion

BD FACS Aria Fusion

  • Fluorescence Activated Cell Sorting
  • 5 spatially separated lasers
  • 18 fluorescent detectors
  • T° control of a sample
  • Sorting on/into device options:
    • Eppendorfs
    • 5ml-, 15ml- tubes
    • 6-, 24-, 48-, 96-, 384-well plates
    • Microscope slides  

More info

 

Elektronová mikroskopie

FEI Helios NanoLab 660 G3 UC

FEI Helios NanoLab 660 G3 UC

  • FIB-SEM DualBeam microscope
  • High resolution scanning electron microscopy with focus ion beam

More info

Leica EM ACE600 – coating system

Leica EM ACE600 – coating system

  • precise coating of samples foe subsequent examination with an electron microscope
  • glow discharge
  • freeze fracture cryo set

More info

Optická mikroskopie

Carl Zeiss LSM 880 NLO

Carl Zeiss LSM 880 NLO

  • Inverted confocal microscope
  • Multiphoton excitation and non-descanned detection with FLIM option
  • Internal spectral detection unit

More info

Leica TCS SP8 WLL SMD-FLIM

Leica TCS SP8 WLL SMD-FLIM

  • Inverted confocal microscope
  • WLL and internal spectral detection unit
  • FLIM and FCS

More info

Nikon N-SIM & N-STORM

Nikon N-SIM & N-STORM

  • Inverted widefield microscope
  • TIRF illumination
  • Image splitter
  • SIM super-resolution microscopy
  • STORM super-resolution microscopy

More info

 

Publikace

2020

Barcytė, D., Pilátová, J., Mojzeš, P. & Nedbalová, L.: The Arctic Cylindrocystis (Zygnematophyceae, Streptophyta) Green Algae are Genetically and Morphologically Diverse and Exhibit Effective Accumulation of Polyphosphate. J. Phycol. 56, 217–232 (2020).

Cifrová, P. et al.: Division of Labor Between Two Actin Nucleators—the Formin FH1 and the ARP2/3 Complex—in Arabidopsis Epidermal Cell Morphogenesis. Front. Plant Sci. 11, 1–17 (2020).

Doubravská, L., Dostál, V., Knop, F., Libusová, L. & Macůrková, M.: Human myotubularin-related protein 9 regulates ER-to-Golgi trafficking and modulates WNT3A secretion. Exp. Cell Res. 386, (2020).

Horníková, L. et al.: The Major Capsid Protein, VP1, of the Mouse Polyomavirus Stimulates the Activity of Tubulin Acetyltransferase 1 by Microtubule Stabilization Lenka. Viruses 12, (2020).

Kim, K. et al.: Novel β- And γ-Amino Acid-Derived Inhibitors of Prostate-Specific Membrane Antigen. J. Med. Chem. 63, 3261–3273 (2020).

Krčová, L. et al.: Highly selective mitochondrial probes based on fluorinated pentamethinium salts: On two-photon properties and microscopic applications. Dye. Pigment. 172, (2020).

Le, T. et al.: Anaerobic peroxisomes in Mastigamoeba balamuthi. Proc. Natl. Acad. Sci. U. S. A. 117, 2065–2075 (2020).

Pramanik, G. et al.: Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications. J. Vis. Exp. (2020) doi:10.3791/60388.

Špaček, M. et al.: Comparison of Different Thawing Protocols in Human Cryopreserved Venous Grafts. Ann. Vasc. Surg. 64, 347–354 (2020).

Šťáhlavský, F. et al.: Cytogenetics of entelegyne spiders (Arachnida, Araneae) from southern Africa. Comp. Cytogenet. 14, 107–138 (2020).

Talianová, V. et al.: Coumarin Tröger’s base derivatives with cyanine substitution as selective and sensitive fluorescent lysosomal probes. Bioorg. Chem. 94, 103447 (2020).

Ustinova, K. et al.: The disordered N-terminus of HDAC6 is a microtubulebinding domain critical for efficient tubulin deacetylation. J. Biol. Chem. 295, 2614–2628 (2020).

Kahancová, A., Sklenář, F., Ježek, P., Dlasková, A.: Overexpression of native IF1 downregulates glucose-stimulated insulin secretion by pancreatic INS-1E cells. Sci. Rep. 10(1) (2020) DOI: 10.1038/s41598-020-58411-x

Sklenář, F. et al.: Increasing the species diversity in the Aspergillus section Nidulantes: Six novel species mainly from the indoor environment. Mycologia 342-370. 2020.

Jankovicova, J., Frolikova, M., Palenikova, E. et al.: Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells. Sci. Rep. 10: 4374 (2020).

Kusari, F., Mihola, O., Schimenti, J.C. and Trachtulec, Z.: Meiotic Epigenetic Factor PRDM9 Impacts Sperm Quality of Hybrid Mice. Reproduction 160:1 (2020) DOI: https://doi.org/10.1530/REP-19-0528

Kristofikova, Z., Springer, T., Gedeonova, E. et al.: Interactions of 17β-Hydroxysteroid Dehydrogenase Type 10 and Cyclophilin D in Alzheimer’s Disease. Neurochemical Research 45:915-927 (2020).

Prasai, A., Schmidt Cernohorska, M., Ruppova, K. et al.: The BBSome assembly is spatially controlled by BBS1 and BBS4 in human cells. Biorxiv (2020) doi: https://doi.org/10.1101/2020.03.20.000091

Vackova, J., Piatakova, A., Polakova, I. and Smahel, M.: Abrogation of IFN-γ Signaling May not Worsen Sensitivity to PD-1/PD-L1 Blockade. Int. J. Mol. Sci. 21(5) (2020) doi.org/10.3390/ijms21051806

2019

Barinka, C. et al.: Structural and computational basis for potent inhibition of glutamate carboxypeptidase II by carbamate-based inhibitors. Bioorganic Med. Chem. 27, 255–264 (2019).

Bohuslavova, R. et al.: HIF-1α is required for development of the sympathetic nervous system. Proc. Natl. Acad. Sci. U. S. A. (2019) doi:10.1073/pnas.1903510116.

Dlasková, A. et al.: Mitochondrial cristae narrowing upon higher 2-oxoglutarate load. Biochim. Biophys. Acta - Bioenerg. 1860, 659–678 (2019).

Fawley, M. W., Němcová, Y. & Fawley, K. P.: Phylogeny and characterization of paraeustigmatos columelliferus, gen. Et sp. nov., a member of the eustigmatophyceae that may represent a basal group within the eustigmatales. Fottea 19, 107–114 (2019).

Figura, T., Tylová, E., Šoch, J., Selosse, M. A. & Ponert, J.: In vitro axenic germination and cultivation of mixotrophic Pyroloideae (Ericaceae) and their post-germination ontogenetic development. Ann. Bot. 123, 625–639 (2019).

Frolikova, M. et al.: Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm. Int. J. Mol. Sci. 20, 1–16 (2019).

Gemperle, J., Dibus, M., Koudelková, L., Rosel, D. & Brábek, J.: The interaction of p130Cas with PKN3 promotes malignant growth. Mol. Oncol. 13, 264–289 (2019).

Hernychová, L. et al.: The C-type lectin-like receptor Nkrp1b: Structural proteomics reveals features affecting protein conformation and interactions. J. Proteomics 196, 162–172 (2019).

Havlík, M. et al.: Versatile fluorophores for bioimaging applications: π-expanded naphthalimide derivatives with skeletal and appendage diversity. Chem. Commun. 55, 2696–2699 (2019).

Kohutova, J. et al.: Anti-arrhythmic cardiac phenotype elicited by chronic intermittent hypoxia is associated with alterations in connexin-43 expression, phosphorylation, and distribution. Front. Endocrinol. (Lausanne). 10, 1–10 (2019).

Koudelkova, L. et al.: Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions. Cell Chem. Biol. 1–14 (2019).

Karnkowska, A. et al.: The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion. Mol. Biol. Evol. (2019) doi:10.1093/molbev/msz147.

Krejcir, R. et al.: A cyclic pentamethinium salt induces cancer cell cytotoxicity through mitochondrial disintegration and metabolic collapse. Int. J. Mol. Sci. 20, (2019).

Makki, A. et al.: Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis. PLOS Biology vol. 17 (2019).

Musilova, Z. et al. Evolution of the visual sensory system in cichlid fishes from crater lake Barombi Mbo in Cameroon. Mol. Ecol. (2019) doi:10.1111/mec.15217.

Oulehlová, D. et al.: Arabidopsis Class I Formin FH1 Relocates between Membrane Compartments during Root Cell Ontogeny and Associates with Plasmodesmata. Plant Cell Physiol. 60, 1855–1870 (2019).

Meiring, J. C. M. et al.: Colocation of Tpm3.1 and myosin IIa heads defines a discrete subdomain in stress fibres. J. Cell Sci. 132, (2019).

Palma-Onetto, V. et al.: The labral gland in termites: Evolution and function. Biol. J. Linn. Soc. 126, 587–597 (2019).

Princová, J., Schätz, M., Ťupa, O. & Převorovský, M.: Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing. J. Vis. Exp. (2019) doi:10.3791/59889.

Pusztai, M. & Škaloud, P.: Elucidating the evolution and diversity of Uroglena-like colonial flagellates (Chrysophyceae): polyphyletic origin of the morphotype. Eur. J. Phycol. 54, 404–416 (2019).

Rada, P., Makki, A., Žárský, V. & Tachezy, J.: Targeting of tail-anchored proteins to Trichomonas vaginalis hydrogenosomes. Mol. Microbiol. 111, 588–603 (2019).

Rada, P., Kellerová, P., Verner, Z. & Tachezy, J.: Investigation of the Secretory Pathway in Trichomonas vaginalis Argues against a Moonlighting Function of Hydrogenosomal Enzymes. J. Eukaryot. Microbiol. 66, 899–910 (2019).

Rubešová, K. et al.: Heavily Ce3+-doped Y3Al5O12 thin films deposited by a polymer sol-gel method for fast scintillation detectors. CrystEngComm 21, 5115–5123 (2019).

Štundlová, J., Šmíd, J., Nguyen, P. & Šťáhlavský, F.: Cryptic diversity and dynamic chromosome evolution in Alpine scorpions (Euscorpiidae: Euscorpius). Mol. Phylogenet. Evol. 134, 152–163 (2019).

Siahaan, V. et al.: Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes. Nat. Cell Biol. 21, 1087- (2019).

Schwarzerová, K. et al.: Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway. Sci. Rep. 9, 1–13 (2019).

Schmidt-Cernohorska, M. et al.: Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. Science. 363, 285–288 (2019).

Treitli, S. C., Kolisko, M., Husník, F., Keeling, P. J. & Hampl, V.: Revealing the metabolic capacity of Streblomastix strix and its bacterial symbionts using singlecell metagenomics. Proc. Natl. Acad. Sci. U. S. A. 116, 19675–19684 (2019).

Vennin, C. et al.: CAF hierarchy driven by pancreatic cancer cell p53-status creates a pro-metastatic and chemoresistant environment via perlecan. Nat. Commun. (2019) doi:10.1038/s41467-019-10968-6.

2018

Bajzikova, M., Kovarova, J., Coelho, A. R. et al.: Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells. Cell Metab (2018).

♯ STED                ♯ Huygens             ♯ confocal       ♯ 2-photon excitation   

Baresova, V., Skopova, V., Souckova, O. et al.: Study of Purinosome Assembly in Cell-based Model Systems with De Novo Purine Synthesis and Salvage Pathway Deficiencies. PLoS One 13, 1-13 (2018).

♯ Huygens      ♯ confocal

Barlow, L. D., Nyvltova, E., Aguilar, M. et al.: A Sophisticated, Differentiated Golgi in the Ancestor of Eukaryotes. BMC Biology 16, 27 (2018).

♯ SIM

Cerychova, R., Bohuslavova, R., Papousek, F et al.: Adverse Effects of Hif1a Mutation and Maternal Diabetes on the Offspring Heart Cardiovas Diabetol 17, 1-16 (2018).

♯ confocal  ♯ SEM

Font-Haro, A. et al.: Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level During ART. Viruses 3, 1-13 (2018).

♯ BD Fortessa

Frolikova, M., Manaskova-Postlerova, P., Cerny, J. et al.: CD9 and CD81 Interactions and their Structural Modelling in Sperm Prior to Fertilization. Intern Journal of Mol Sci 19, 4-17 (2018).

♯ SIM  ♯ confocal      ♯ Huygens

Grzelak, A., Polakova, I., Smahelova, J. el al.: Experimental Combined Immunotherapy of Tumours with Major Histocompatibility Complex Class I Downregulation. Intern Journal of Molec Sci 19, 3693 (2018).

♯ BD Fortessa

Hubackova, S., Davidova, E., Rohlenova, K. et al.: Selective Elimination of Senescent Cells by Mitochondrial Targeting is Regulated by ANT2. Cell Death and Differ, 1-15 (2018).

♯ BD Fortessa

Janovec, V., Aouar, B., Font-Haro, A. et al.: The MEK1/2-ERK Pathway Inhibits Type I IFN Production in Plasmacytoid Dendritic Cells. Front In Immunol 9, 1-16 (2018).

♯ BD Aria       ♯ BD Fortessa

Kazamia, E., Sutak, R., Paz-Yepes, J. et al.: Endocytosis-mediated Siderophore Uptake as a Strategy for Fe Acquisition in Diatoms. Science Advances 4 (2018).

♯ confocal

Kollarova, J., Kostrouchova, M., Benda, A. and Kostrouchova, M.: ALKB-8, a 2-Oxoglutarate-Dependent Dioxygenase and S-Adenosine Methionine-Dependent Methyltransferase Modulates Metabolic Events Linked to Lysosome-Related Organelles and Aging in C. elegans. Folia Biol. (Praha). 64, 46–58 (2018).

♯ FLIM

Kostrouchova, M., Kostrouchova, V., Yilma, P. et al.: Valproic Acid Decreases the Nuclear Localization of MDT-28, the Nematode Orthologue of MED28. Folia Biologica 64, 1-19 (2018).

♯ FLIM

Mach, J., Bila, J., Zeniskova, K. et al.: Iron Economy in Naegleria Gruberi Reflects its Metabolic Flexibility. Intern Journal for Parasitol 48, 719-727 (2018).

♯ Huygens      ♯ confocal

Rada, P., Makki, A., Zarsky, V. and Tachezy, J.: Targeting of Tail-Anchored Proteins to Trichomonas Vaginalis Hydrogenosomes. Mol Microbiol (2018).

♯ SIM              ♯ STED           ♯ confocal       ♯ EM chemical fixation

Stafkova, J., Rada, P., Meloni, D. et al.: Dynamic Secretome of Trichomonas Vaginalis : Case Study of β-amylases. Mol & Cell Proteom 17, 304-320 (2018).

♯ SIM             ♯ TEM

Becker, W., Studier, H., Wetzker, C. and Benda, A.: Ultra-fast HPM Detectors Improve NAD(P)H FLIM. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 10498 (2018).

♯ 2-photon excitation ♯ FLIM

Ma, Y., Benda, A., Kwiatek, J. et al.: Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels. Biophys Journal (2018).

♯ FLIM  ♯ time-resolved GP

Nicovich, P. R., Kwiatek, J. M., Ma, Y. et al.: FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells. Biophys Jour 114, 2855-2864 (2018).

♯ FCS

Olsinova, M., Jurkiewicz, P., Kishko, I. et al.: Roughness of transmembrane helices reduces lipid membrane dynamics. iScience 10, 87–97 (2018).

♯ FCS

Proksova, P., Lipov, J., Zelenka, J. et al.: Mason-Pfizer Monkey Virus Envelope Glycoprotein Cycling and Its Vesicular Co-Transport with Immature Particles. Viruses 10, 575 (2018).

# spinning disk           # TEM            # widefield

Shestivska, V., Olsinova, M., Sovova, K. et al.: Evaluation of Lipid Peroxidation by the Analysis of Volatile Aldehydes in the Headspace of Synthetic Membranes Using Selected Ion Flow Tube Mass Spectrometry. Rapid Commun in Mass Spectrometry 32, 1617-1628 (2018).

Allolio, C., Magarkar, A., Jurkiewicz, P. et al.: Arginine-rich Cell-penetrating Peptides Induce Membrane Multilamellarity and Subsequently Enter via Formation of a Fusion Pore. PNAS (2018), 201811520.

♯ FIB-SEM     ♯ FLIM

Cermak, V. et al.: RNA-seq of Macrophages of Amoeboid or Mesenchymal Migratory Phenotype Due to Specific Structure of Environment. Sci. Data 5, 1–8 (2018).

Gemperle, J., Dibus, M., Koudelkova, L., Rosel, D. and Brabek, J.: The Interaction of p130Cas with PKN3 Promotes Malignant Growth. Mol. Oncol. 1–26 (2018).

♯ confocal

Koudelkova, L. et al.: Novel FRET-Based Src Biosensor Reveals Mechanisms of Src Activation and Its Dynamics in Focal Adhesions. Cell Chem. Biol. 1–14 (2018).

♯ confocal       ♯ TIRF            ♯ FRET           ♯ FRAP

Siahaan, V. et al.: Kinetically Distinct Phases of Tau on Microtubules Regulate Kinesin Motors and Severing Enzymes. BioRxiv (2018). doi:10.1101/424374

♯ TIRF

Soldatova, I., Prilepskaja, T., Abrahamyan, L., Forstova, J. and Huerfano, S.: Interaction of the Mouse Polyomavirus Capsid Proteins with Importins is Required for Efficient Import of Viral DNA Into the Cell Nucleus. Viruses 10, 1–22 (2018).

♯ confocal

Tolde, O. et al.: Quantitative Phase Imaging Unravels New Insight Into Dynamics of Mesenchymal and Amoeboid Cancer Cell Invasion. Sci. Rep. 8, 1–13 (2018).

♯ hologpraphy

Agarwal, K. Machan, R. and Prasad, D.K: Non-heuristic Automatic Techniques for Overcoming Low Signal-to-Noise-Ratio bias of Localization Microscopy and Multiple Signal Classification Algorithm. Scientific Repors 8 (1), 2018.

♯ Elyra

Neburkova, J., Sedlak. F., Zackova Suchanova, J. et al.: Inhibitor-GCPII Interaction: Selective and Robust System for Targeting Cancer Cells with Structurally Diverse Nanoparticles. Mol Pharmaceut, 15 (8), 2932 – 2945 (2018).

♯ BD Fortessa

2017

Blecha, J. et al.: Antioxidant Defense in Quiescent Cells Determinates Selectivity of Electron Transport Chain Inhibition-Induced Cell Death. Free Radic Biol Med 112, 253-266 (2017).

♯ 2-photon excitation ♯ confocal       ♯ Huygens

Bohuslavova, R., Cerychova, R., Nepomucka, K. and Pavlinkova, G.: Renal injury is accelerated by global hypoxia-inducible factor 1 alpha deficiency in a mouse model of STZ-induced diabetes. BMC Endocr. Disord. 17, 1–12 (2017).

♯ confocal

Branis, J. et al.: The Role of Focal Adhesion Anchoring Domains of CAS in Mechanotransduction. Sci Rep 7 (2017).

♯ STED           ♯ confocal

Danko, M., Hrdlovic, P., Martinicka, A., Benda, A. and Cigan, M.: Spectral Properties of Ionic Benzotristhiazole Based Donor-Acceptor NLO-phores in Polymer Matrices and their one- and two-photone Cellular Imaging Ability. Photochem Photobiol Sci 16 (2017).

♯ 2-photon excitation   ♯ FLIM

Hornikova, L., Fraiberk, M., Man, P., Janovec, V., Forstova, J.: VP1, the Major Capsid Protein of the Mouse Polyomavirus, Binds Microtubules, Promotes their Acetylation and Block the Host Cell Cycle. FEBS J. 284, 301-323 (2017).

♯ BD Fortessa            ♯ TEM            ♯ confocal

Huerfano, S., Ryabchenko, B., Spanielova, H. and Forstova, J.: Hydrophobic Domains of Mouse Polyomavirus Minor Capsid Proteins Promote Membrane Association and Virus Exit from the ER.  FEBS J 284, 883-902 (2017).

♯ confocal       ♯ TEM

Kellerova, P. and Tachezy, J.: Zootonic Trichomonas Tenas and the New Trichomonad Species, Trichomonas Brixi n.sp., from the Oral Cavities of Dogs and Cats. Int J Parasitol 47, 247-255 (2017).

♯ SIM              ♯ FIB-SEM                 ♯ TEM

Kokavec, J. et al.: The ISWI ATPase Smarca5 (Snf2h) is Required for Proliferation and Differenciation of Hematopoietic Stem and Progenitor Cells. Stem Cells 35, 1614-1623 (2017).

♯ cytometry

Kostrouchova, M. et al.: The Nematode Homologue of Mediator Complex Subunit 28 , F28F8.5, is a Critical Regulator of C. elegans Development. PeerJ 2017 (2017).

♯ FLIM

Luo, W. et al.: ARHGAP42 is Activated by Src-Mediated Tyrosine Phosphorylation to Promote Cell Motility . J Cell Sci 130, 2382-2393 (2017).

♯ confocal

Novakova, Z. et al.: Novel Monoclonal Antibodies Recognizing Human Prostate-Specific Membrane Antigen (PSMA) as Research and Theranostic Tools. Prostate 77, 749–764 (2017).

♯ BD Fortessa 

Pavlinkova, G. et al.: Transgenerational inheritance of susceptibility to diabetes-induced male subfertility. Sci. Rep. 7, (2017).

♯ confocal

Skultetyova, L. et al.: Human Histone Deacetylase 6 Shows Strong Preference for Tubulin Dimers Over Assebled Microtubules. Sci Rep 7 (2017).

♯ TIRF

Voleman, L. et al.: Giardia Intestinalis Mitosomes Undergo Synchronised Fission but not Fusion and are Constitutively Associated with the Endoplasmic Reticulum. BMC Biol 15 (2017).

♯ SIM             ♯ widefield     ♯ spinning disk           ♯ Huygens

Zackova Suchanova, N., Neburkova, J., Spanielova, H., Forstova, J. and Cigler, P.: Retargeting Polyomavirus-like Particles to Cancer Cells by Chemical Modification of Capsid Surface. Bioconug Chem. 28, 307-313 (2017).

♯ BD Fortessa            ♯ TEM            ♯ confocal      

2016

 Boukalova, S. et al.: Mitochondrial Targeting of Metformin Enhances its Activity against Pancreatic Cancer. Mol Cancer Ther. 15, 2875-2886 (2016).

♯ BD Fortessa

Dvorakova, M. et al.: Incomplete and Delayed Sox2 Deletion Defines Residual Ear Neurosensory Development and Maintenance. Sci Rep 6, 1-16 (2016).

♯ confocal       ♯ SEM

Jankovicova, J. et al.: Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes. Reproduction (2016). doi:10.1530/REP-16-0304

♯ confocal       ♯ Huygens

Agarwal, K. & Macháň, R. Multiple signal classification algorithm for super-resolution fluorescence microscopy. Nat. Commun. 7, (2016).

♯ Superresolution microscopy

Benda, A., Aitken, H., Davies, D. S., Whan, R. & Goldsbury, C. STED imaging of tau filaments in Alzheimer’s disease cortical grey matter. J. Struct. Biol. 195, (2016).

♯ STED

Jiang, Y. et al. Time-resolved fluorescence anisotropy study of organic lead halide perovskite. Sol. Energy Mater. Sol. Cells 151, (2016).

Ma, Y., Benda, A., Nicovich, P. R. & Gaus, K. Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy. Biomed. Opt. Express 7, (2016).

Macháň, R., Foo, Y. H. & Wohland, T. On the Equivalence of FCS and FRAP: Simultaneous Lipid Membrane Measurements. Biophys. J. 111, 152–161 (2016).

Yavas, S., Macháň, R. & Wohland, T. The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells. Biophys. J. 111, 2241–2254 (2016).

Tým

Mgr. Aleš Benda, Ph.D. Mgr. Aleš Benda, Ph.D.

Mgr.
Aleš Benda, Ph.D.

Head of the facility, optical (fluorescence) microscopy

ales.benda@natur.cuni.cz
+420325873940
Mgr. Marie Olšinová, Ph.D Mgr. Marie Olšinová, Ph.D

Mgr.
Marie Olšinová, Ph.D

Optical (fluorescence) microscopy

marie.olsinova@natur.cuni.cz
+420325873923
Ing. Dalibor Pánek, Ph.D. Ing. Dalibor Pánek, Ph.D.

Ing.
Dalibor Pánek, Ph.D.

Optical (fluorescence) microscopy

dalibor.panek@natur.cuni.cz
+420325873940
Mgr. Irena Krejzová, Ph.D. Mgr. Irena Krejzová, Ph.D.

Mgr.
Irena Krejzová, Ph.D.

Electron microscopy

irena.krejzova@natur.cuni.cz
+420325873923
Mgr. Martin Fraiberk, Ph.D Mgr. Martin Fraiberk, Ph.D

Mgr.
Martin Fraiberk, Ph.D

Electron microscopy

martin.fraiberk@natur.cuni.cz
+420325873924
Mgr. Lucia Motlová Mgr. Lucia Motlová

Mgr.
Lucia Motlová

Electron microscopy

lucia.motlova@natur.cuni.cz
+420325873924
Bc. Lenka Backová Bc. Lenka Backová

Bc.
Lenka Backová

Data analysis

lenka.backova@natur.cuni.cz
Mgr. Ján Sabó Mgr. Ján Sabó

Mgr.
Ján Sabó

Optical (fluorescence) microscopy

jan.sabo@natur.cuni.cz
+420325873924
Mgr. Libuše Vaněčková Mgr. Libuše Vaněčková

Mgr.
Libuše Vaněčková

Administration support

turjanic@natur.cuni.cz
+420325873940
Ing. Galina Kislik Ing. Galina Kislik

Ing.
Galina Kislik

Flow Cytometry – on maternity leave

galina.kislik@natur.cuni.cz
+420325873940
Mgr. Markéta Dalecká Mgr. Markéta Dalecká

Mgr.
Markéta Dalecká

Electron microscopy – on maternity leave

daleckama@natur.cuni.cz
+420325873923
Ing. Petra Prokšová-Grznárová, Ph.D. Ing. Petra Prokšová-Grznárová, Ph.D.

Ing.
Petra Prokšová-Grznárová, Ph.D.

Flow cytometry and Optical (fluorescence) microscopy - on maternity leave

petra.grznarova@natur.cuni.cz