Intravital inverted two-photon and confocal microscope with 34 channel spectral detection and with four reflected and two transmitted NDD detectors (including two highly sensitive GaAsP detectors suitable for FLIM), equipped with MP laser with OPO and with the full set of visible cw lasers.
Carl Zeiss AxioObserver.Z1 with confocal module LSM 880 NLO and MP excitation
LSM 880 NLO offers many imaging modes – except the classic scanning confocal mode, the system is equipped by 32-channels detector for spectral detection and software to separate probes with very similar spectra (e.g. GFP and YFP) and also by multiphoton laser with OPO, so tuning of wavelengths in range 690 nm – 1300 nm is possible. The part of the multiphoton light path is also motorized periscope for fine adjusting/focusing of MP beam into the scanning head of the microscope. Detection part of multiphoton light path contains NDD.2 module with 2 standard PMT detectors and BIG.2 module with 2 GaAsP PMT detectors. The system has wide range of 1P excitation lasers – 405, 458, 488, 514, 561 and 633 nm, epifluorescence light source (metal halide lamp) and GFP and RFP fluorescence filter sets for eyepiece detection of signal, wide range of objectives – 5x, 10x, 25x, 40x, 63x water immersion, 63x oil immersion. For detection of transmitted light, the microscope has another NDD.2 module with 2 detectors. The source for transmitted light is VIS-LED. Insert with controlled temperature is also available with the system.
Whole system is controlled by ZEN Black with 3D visualization, FRAP Efficiency, Tiles and Positions, Experiment designer license keys.
- Deep imaging of thick biological samples due to the multiphoton excitation
- Spectral detection, spectral unmixing and fingerprinting
- Confocal imaging
- SHG, THG
405nm, CW, 30mW
Argon laser: 458, 488, 514nm, 25mW
EC Plan-Neofluar 5x, NA 0.16, WD 18.5mm
Plan-Apochromat 10x, NA 0.45, WD 2.1mm
LD LCI Plan-Apochromat 25x Correction collar, NA 0.8, multi-immersion: water, oil, glycerin, WD0.57 at D=0.17 (correction for coverglass thickness D=0-0.17), DIC
LD C-Apochromat 40x Correction collar, NA 1.1, water immersion, D=0.14-0.19, WD0.62 at D=0.17, DIC
C-Apochromat 63x Correction collar, NA 1.2, water immersion, D=0.14-0.19, WD0.28 at D=0.17, DIC
Plan-Apochromat 63x, NA 1.4, oil immersion, WD 0.19mm, DIC
Epifluorescence filter sets
- Filter set GFP: Ex BP 470/40, BS FT 495, EM BP 525/50
- Filter set Cy3: Ex BP 545/25, BS FT 570, EM BP 605/70
Out of the system:
- Filter set DAPI: EX G 365, BS FT 395, EM BP 445/50
- Filter set Cy5: Ex BP 640/30, BS FT 660, EM BP 690/50