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Imaging Methods

About us

Introduction

IMCF covers three main areas-optical (fluorescence) microscopy, electron microscopy and flow cytometry. We offer our users training on and access to high end instrumentation, plus we provide consulting services on optimal experimental design, sample preparation, data acquisition and analysis and interpretation of the obtained results.

 

Euro-Bioimaging

IMCF BIOCEV is part of the Czech node of the Euro-BioImaging infrastructure.

Euro-BioImaging

Czech-Bioimaging

We also participate in the Czech-BioImaging national infrastructure.

czecheurobioimaging logo

Reservation

booking_icon

 

 

 

Instruction how to use the booking systém are here (Alternate login instructions).

In case of any questions, feel free to contact the particular scientist.

Prices

The pricelist of optical (fluorescence) techniques for the external users.

For the pricelists of flow cytometry and electron microscopy contact Petra Grznarova and Adam Schröfel, respectively.

Team and contacts

Benda_ales
  • Aleš Benda, M.Sc.,Ph.D.

  • Head of the facility, optical (fluorescence) microscopy
  • +420 325 873 940
  • +420 735 196 355
Místnost L1.013
Místnost
L1.014
Místnost
L1.014
  • Adam Schröfel, Ph.D.

  • Electron microscopy, correlative light and electron microscopy
  • +420 325 873 924
  • +420 735 196 352
Místnost
M1.003

Where to find us in BIOCEV center

ImagingMethods-plan

Mapa1NP-ZM


Equipment


  • Carl Zeiss LSM 880 NLO

    Intravital inverted two-photon and confocal microscope with 34 channel spectral detection and with four reflected and two transmitted NDD detectors (including two highly sensitive GaAsP detectors suitable for FLIM), equipped with MP laser with OPO and with the full set of visible cw lasers.

  • FEI Helios NanoLab G3 UC–FIB-SEM

    Scanning electron microscope with focused ion beam milling equipped for cryo-imaging and correlative light and electron microscopy (CLEM) plus accessories (Leica EM HPM100, ULTRACUT UC7 with FC7, AFS2, CPD300 A and KMR3) for sample preparation.

  • BD FACS Aria Fusion

    (5 lasers, 19 detectors, jet-in-air 4-way sorter, 70 000 cells per minute) and BD LSR Fortessa SORP (5 non-collinear lasers, 20 detectors, 40 000 samples per second) for complex fluorescence flow cytometry analysis and sorting.

  • Super-resolution Nikon Ti-E microscopes with N-SIM and N-STORM modules and Nikon Ti-E with STED module from Abberior Instruments

    Each module allows for two-colour 3D super-resolution acquisition, using different approaches to break the diffraction limit of optical resolution.

  • Leica TCS SP8 DM6 CFS and Leica DMi8

    Upright confocal and inverted wide-field microscopes for combined optical and electro-physiological measurements, Nikon Ti-E with Laser H-TIRF System for long term live cell observations and Carl Zeiss AxioZoom.V16 for large specimen observation.

  • Leica TCS SP8 WLL SMD-FLIM

    Inverted confocal microscope with a fluorescence lifetime imaging module (FLIM), including pulsed white light laser (470 nm – 670 nm), pulsed 405 nm laser and freely spectrally selectable detection with two high sensitivity HyD detectors.

Our Services

CF SERVICES – SUMMARY

  • Optical (fluorescence) microscopy
  • Electron microscopy
  • Flow cytometry

 

CF SERVICES

In fluorescence microscopy, we cover the following methods: wide-field and confocal microscopy, two-photon deep intravital microscopy, 3D two-colour super-resolution microscopy (STED, PALM, STORM, SIM), functional imaging (spectral, FLIM, FCS), TIRF, live cell imaging and combined electrophysiological and optical acquisition. In electron microscopy, we offer 3D SEM-FIB acquisition on embedded, dried or frozen samples, elemental analysis (EDS) and combined light and electron microscopy (CLEM) approaches. In flow cytometry, we offer fluorescence activated cell sorting (FACS) and analysis. The strength of our facility is the application, implementation and development of advanced fluorescence imaging techniques, especially super-resolution and functional (FLIM, FCS) imaging.

References and Publications

CONNECTION TO NATIONAL AND INTERNATIONAL RESEARCH INFRASTRUCTURES OR CONSORTIA

IMCF BIOCEV is part of the Czech node of the Euro-BioImaging infrastructure.

Euro-BioImaging

 

The Imaging Methods Core Facility BIOCEV is supported from the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project “National Infrastructure for Biological and Medical Imaging (Czech-BioImaging – LM2015062)”

Ministry of Education, Youth and Sports

POTENTIAL FOR COOPERATION

We are seeking for key partners with biological,  medical and also material science projects that could benefit from custom optimized advanced imaging techniques. We also welcome industrial partners not only interested in using our high-tech equipment, but also partners needing a test facility for their new products and partners interested in commercialisation of our newly developed methods from the field of super-resolution and functional imaging.